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Efficacy of albendazole combined with a marine fungal extract (m2-9) against Angiostrongylus cantonensis-induced meningitis in mice

Published online by Cambridge University Press:  04 November 2011

Z.-Y. Li ,
R. Sun ,
J. Li ,
Y.-X. Song ,
Y.-C. Lin ,
X. Zeng ,
H.-J. He ,
J. Wei ,
F. Yang  and
H.-Q. Zheng
...Show all authors
Z.-Y. Li
Affiliation:
Key Laboratory of Tropical Diseases and Control of the Ministry of Education, Guangzhou, China Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, P.R. China
R. Sun
Affiliation:
Key Laboratory of Tropical Diseases and Control of the Ministry of Education, Guangzhou, China Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, P.R. China
J. Li
Affiliation:
Guangdong Province Key Laboratory of Functional Molecules in Oceanic Microorganisms, Sun Yat-sen University, Guangzhou, P.R. China
Y.-X. Song
Affiliation:
Guangdong Province Key Laboratory of Functional Molecules in Oceanic Microorganisms, Sun Yat-sen University, Guangzhou, P.R. China
Y.-C. Lin
Affiliation:
Guangdong Province Key Laboratory of Functional Molecules in Oceanic Microorganisms, Sun Yat-sen University, Guangzhou, P.R. China
X. Zeng
Affiliation:
Key Laboratory of Tropical Diseases and Control of the Ministry of Education, Guangzhou, China Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, P.R. China
H.-J. He
Affiliation:
Key Laboratory of Tropical Diseases and Control of the Ministry of Education, Guangzhou, China Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, P.R. China
J. Wei
Affiliation:
Key Laboratory of Tropical Diseases and Control of the Ministry of Education, Guangzhou, China Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, P.R. China
F. Yang
Affiliation:
Key Laboratory of Tropical Diseases and Control of the Ministry of Education, Guangzhou, China Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, P.R. China
H.-Q. Zheng
Affiliation:
Key Laboratory of Tropical Diseases and Control of the Ministry of Education, Guangzhou, China Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, P.R. China
Z.-Y. Lv
Affiliation:
Key Laboratory of Tropical Diseases and Control of the Ministry of Education, Guangzhou, China Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, P.R. China
Z.-D. Wu*
Affiliation:
Key Laboratory of Tropical Diseases and Control of the Ministry of Education, Guangzhou, China Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, P.R. China
*
* E-mail: [email protected]
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Abstract

The pathogenesis of angiostrongyliasis, resulting from Angiostrongylus cantonensis invasion of the human central nervous system, remains elusive. Anthelmintics are usually used to kill worms, although dead worms in the brain may cause severe inflammation which will lead to central nervous system damage. Therefore, combination therapy with anthelmintics and anti-inflammatory drugs in the treatment of human angiostrongyliasis needs further study. To evaluate the efficacy of albendazole combined with a marine fungal extract (m2-9) in A. cantonensis infection, BALB/c mice infected by the third-stage larvae of A. cantonensis were divided into three groups: mice treated with albendazole or m2-9 alone or in combination from day 5 post-inoculation (PI). Several efficacy parameters were recorded, including weight change, worm recovery, neurological function, behavioural analysis, eosinophil and leucocyte counts. The results showed that combination therapy increased body weight, reduced worm burden, improved learning ability, memory and action, decreased neurological dysfunction and leucocyte response in these mice. The combination of albendazole and m2-9 treatment significantly decreased leucocyte response and increased the frequency of rearing, compared to infected mice treated with either drug alone. Therefore, m2-9 is a natural product with potentially significant therapeutic value for angiostrongyliasis and is worthy of further study.

Type
Research Papers
Information
Journal of Helminthology , Volume 86 , Issue 4 , December 2012 , pp. 410 - 417
Copyright
Copyright © Cambridge University Press 2011

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References

Aiello, A., D'Esposito, M., Fattorusso, E., Menna, M., Muller, W.E., Perović-Ottstadt, S. & Schröder, H.C. (2006) Novel bioactive bromopyrrole alkaloids from the Mediterranean sponge Axinella verrucosa. Bioorganic & Medicinal Chemistry 14, 17–24.CrossRefGoogle ScholarPubMed
Alicata, J.E. (1965) Occurrence of Angiostrongylus cantonensis in Madagascar, Mauritius, Ceylon, and Sarawak. Journal of Parasitology 51, 937.CrossRefGoogle ScholarPubMed
Andersen, R.J. & Williams, D.E. (2000) Pharmaceuticals from the sea. pp. 55in (Eds) Chemistry in the marine environment. Cambridge, The Royal Society of Chemistry Press.Google Scholar
Bittencourt, F.S., Figueiredo, J.G., Mota, M.R., Bezerra, C.C., Silvestre, P.P., Vale, M.R., Nascimento, K.S., Sampaio, A.H., Nagano, C.S., Saker-Sampaio, S., Farias, W.R., Cavada, B.S., Assreuy, A.M. & de Alencar, N.M. (2008) Antinociceptive and anti-inflammatory effects of a mucin-binding agglutinin isolated from the red marine alga Hypnea cervicornis. Naunyn-Schmiedeberg's Archive of Pharmacology 377, 139–148.CrossRefGoogle Scholar
Bugni, T.S. & Ireland, C.M. (2004) Marine derived fungi: a chemically and biologically diverse group of microorganisms. Natural Product Reports 21, 143–163.CrossRefGoogle ScholarPubMed
Chen, H.T. (1935) Un nouveau nematode pulmonaire, Pulmonema cantonensis n.g., n. sp. des rats de Canton. Annals of Parasitology 13, 312–317.Google Scholar
Chen, K.M. & Lai, S.C. (2007) Biochemical and pathological evaluation of albendazole/thalidomide co-therapy against eosinophilic meningitis or meningoencephalitis induced by Angiostrongylus cantonensis. Journal of Antimicrobial Chemotherapy 59, 264–276.CrossRefGoogle ScholarPubMed
Courtois, A., Simon-Colin, C., Boisset, C., Berthou, C., Deslandes, E., Guézennec, J. & Bordron, A. (2008) Floridoside extracted from the red alga Mastocarpus stellatus is a potent activator of the classical complement pathway. Marine Drugs 6, 407–417.CrossRefGoogle ScholarPubMed
Cross, J.H. (1978) Clinical manifestations and laboratory diagnosis of eosinophilic meningitis syndrome associated with angiostrongyliasis. Southeast Asian Journal of Tropical Medicine and Public Health 9, 161–170.Google ScholarPubMed
Dang, H.T., Lee, H.J., Yoo, E.S., Shinde, P.B., Lee, Y.M., Hong, J., Kim, D.K. & Jung, J.H. (2008) Anti-inflammatory constituents of the red alga Gracilaria verrucosa and their synthetic analogues. Journal of Natural Products 71, 232–240.CrossRefGoogle ScholarPubMed
Faulkner, D.J. (2002) Marine natural products. Natural Product Reports 19, 1–48.Google ScholarPubMed
Gelis, S., Spratt, D.M. & Raidal, S.R. (2011) Neuroangiostrongyliasis and other parasites in tawny frogmouths (Podargus strigoides) in south-eastern Queensland. Australian Veterinary Journal 89, 47–50.CrossRefGoogle ScholarPubMed
Hsieh, H.C. (1959) Outline of parasitic zoonoses in Taiwan. Formosan Science 13, 99–108.Google Scholar
Hwang, K.P. & Chen, E.R. (1988) Larvicidal effect of albendazole against Angiostrongylus cantonensis in mice. American Journal of Tropical Medicine and Hygiene 39, 191–195.CrossRefGoogle ScholarPubMed
Ina, A., Hayashi, K., Nozaki, H. & Kamei, Y. (2007) Pheophytin a, a low molecular weight compound found in the marine brown alga Sargassum fulvellum, promotes the differentiation of PC12 cells. International Journal of Developmental Neuroscience 25, 63–68.CrossRefGoogle ScholarPubMed
John, D.T. & Martinez, A.J. (1975) Animal model of human disease. Central nervous system infection with the nematode Angiostrongylus cantonensis. Animal model: eosinophilic meningoencephalitis in mice infected with Angiostrongylus cantonensis. The American Journal of Pathology 80, 345–348.Google ScholarPubMed
König, G.M. & Wright, A.D. (1996) Marine natural products research: current directions and future potential. Planta Medica 62, 193–211.CrossRefGoogle ScholarPubMed
Lai, S.C., Chen, K.M., Chang, Y.H. & Lee, H.H. (2008) Comparative efficacies of albendazole and the Chinese herbal medicine long-dan-xie-gan-tan, used alone or in combination, in the treatment of experimental eosinophilic meningitis induced by Angiostrongylus cantonensis. Annals of Tropical Medicine and Parasitology 102, 143–150.CrossRefGoogle ScholarPubMed
Laird, D.W. & van Altena, I.A. (2006) Tetraprenyltoluquinols from the brown alga Cystophora fibrosa. Phytochemistry 67, 944–955.CrossRefGoogle ScholarPubMed
Lan, K.P., Wang, C.J., Lai, S.C., Chen, K.M., Lee, S.S., Hsu, J.D. & Lee, H.H. (2004) The efficacy of therapy with albendazole in mice with parasitic meningitis caused by Angiostrongylus cantonensis. Parasitology Research 93, 311–317.CrossRefGoogle ScholarPubMed
Lee, H.H., Chou, H.L., Chen, K.M. & Lai, S.C. (2004) Association of matrix metalloproteinase-9 in eosinophilic meningitis of BALB/c mice caused by Angiostrongylus cantonensis. Parasitology Research 94, 321–328.CrossRefGoogle ScholarPubMed
Li, X., Lee, S.M., Choi, H.D., Kang, J.S. & Son, B.W. (2003) Microbial transformation of terreusinone, an ultraviolet-A (UV-A) protecting dipyrroloquinone, by Streptomyces sp. Chemical & Pharmaceutical Bulletin 51, 1458–1459.CrossRefGoogle ScholarPubMed
Liberra, K. & Lindequist, U. (1995) Marine fungi – a prolific resource of biologically active natural products? Pharmazie 50, 583–588.Google ScholarPubMed
Maldonado, A. Jr, Simões, R.O., Oliveira, A.P., Motta, E.M., Fernandez, M.A., Pereira, Z.M., Monteiro, S.S., Torres, E.J. & Thiengo, S.C. (2010) First report of Angiostrongylus cantonensis (Nematoda: Metastrongylidae) in Achatina fulica (Mollusca: Gastropoda) from Southeast and South Brazil. Memórias do Instituto Oswaldo Cruz 105, 938–941.CrossRefGoogle ScholarPubMed
Mayer, A.M. & Gustafson, K.R. (2004) Marine pharmacology in 2001–2: antitumour and cytotoxic compounds. European Journal of Cancer 40, 2676–2704.CrossRefGoogle ScholarPubMed
Oh, D.C., Gontang, E.A., Kauffman, C.A., Jensen, P.R. & Fenical, W. (2008) Salinipyrones and pacificanones, mixed-precursor polyketides from the marine actinomycete Salinispora pacifica. Journal of Natural Products 71, 570–575.CrossRefGoogle ScholarPubMed
Parra, A., McGirt, M.J., Sheng, H., Laskowitz, D.T., Pearlstein, R.D. & Warner, D.S. (2002) Mouse model of subarachnoid hemorrhage associated cerebral vasospasm: methodological analysis. Neurological Research 24, 510–516.CrossRefGoogle ScholarPubMed
Pietra, F. (1997) Secondary metabolites from marine microorganisms: bacteria, protozoa, algae and fungi. Achievements and prospects. Natural Product Reports 14, 453–464.CrossRefGoogle ScholarPubMed
Ponomarenko, L.P., Kalinovsky, A.I., Afiyatullov, S.Sh., Pushilin, M.A., Gerasimenko, A.V., Krasokhin, V.B. & Stonik, V.A. (2007) Spongian diterpenoids from the sponge Spongia (Heterofibria) sp. Journal of Natural Products 70, 1110–1113.CrossRefGoogle ScholarPubMed
Sawanyawisuth, K. & Sawanyawisuth, K. (2008) Treatment of angiostrongyliasis. Transactions of the Royal Society of Tropical Medicine and Hygiene 102, 990–996.CrossRefGoogle ScholarPubMed
Tu, W.C. & Lai, S.C. (2006) Angiostrongylus cantonensis: efficacy of albendazole–dexamethasone co-therapy against infection-induced plasminogen activators and eosinophilic meningitis. Experimental Parasitology 113, 8–15.CrossRefGoogle ScholarPubMed
Venkatesan, P. (1998) Albendazole. Journal of Antimicrobial Chemotherapy 41, 145–147.CrossRefGoogle ScholarPubMed
Wang, J., Qi, H., Diao, Z., Zheng, X., Li, X., Ma, S., Ji, A. & Yin, C. (2010) An outbreak of angiostrongyliasis cantonensis in Beijing. Journal of Parasitology 96, 377–381.CrossRefGoogle ScholarPubMed
Wang, L.C., Jung, S.M., Chen, C.C., Wong, H.F., Wan, D.P. & Wan, Y.L. (2006) Pathological changes in the brains of rabbits experimentally infected with Angiostrongylus cantonensis after albendazole treatment: histopathological and magnetic resonance imaging studies. Journal of Antimicrobial Chemotherapy 57, 294–300.CrossRefGoogle ScholarPubMed
Wang, Q.P., Lai, D.H., Zhu, X.Q., Chen, X.G. & Lun, Z.R. (2008) Human angiostrongyliasis. Lancet Infectious Diseases 8, 621–630.CrossRefGoogle ScholarPubMed
Yii, C.Y. (1976) Clinical observations on eosinophilic meningitis and meningoencephalitis caused by Angiostrongylus cantonensis on Taiwan. American Journal of Tropical Medicine and Hygiene 25, 233–249.CrossRefGoogle ScholarPubMed