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Effects of temperature on survival, infectivity and in vitro encystment of the cercariae of Echinostoma caproni

Published online by Cambridge University Press:  12 April 2024

B. Fried  and
E.L. Ponder
B. Fried*
Affiliation:
Department of Biology, Lafayette College, Easton, PA 18042, USA
E.L. Ponder
Affiliation:
Department of Biology, Lafayette College, Easton, PA 18042, USA
*
*Fax: 610 330 5705 E-mail: [email protected]
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Abstract

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The effects of temperature on survival, infectivity and in vitro encystment of Echinostoma caproni cercariae in artificial spring water (ASW) were studied. Effects of aging cercariae in ASW at various temperatures showed that at 23°C cercariae achieved 50% survival in 24 h, compared to 92 h at 12°C. Cercariae aged in ASW at 28 and 37.5°C showed 50% survival at 16 and 10 h, respectively. Cercariae aged at different temperatures for various times were used to infect juvenile Helisoma trivolvis (Colorado strain) snails maintained in ASW at 23°C. Index of infectivity was based on counting encysted metacercariae in the snails at 8 to 12 h post-infection. Cercariae aged at 23, 28 and 37.5°C showed 50% encystment at 6, 8 and 4 h, respectively. Cercariae aged at 4°C showed 50% encystment in 10 h and cercariae aged at 12°C showed 50% encystment beyond 16 h. Cercariae showed maximal longevity and infectivity in snails when aged at 12°C in ASW. For E. caproni, as in other digeneans, the infective period of cercariae is markedly shorter than the maximal life-span at any given temperature. Studies on in vitro encystment of E. caproni cercariae in Locke's solution:ASW (1:1) showed that encystment was optimal at 23°C (78% encystment) and that it declined to 44% at 28°C and became almost nil (0.02%) at 12 or 37.5°C.

Type
Review Article
Information
Journal of Helminthology , Volume 77 , Issue 3 , September 2003 , pp. 235 - 238
Copyright
Copyright © Cambridge University Press 2003

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