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Effects of recombinant bovine interferon γ on Strongyloides papillosus infection in calves

Published online by Cambridge University Press:  12 April 2024

Y. Nakamura*
Affiliation:
Shichinohe Research Unit, National Institute of Animal Health, Shichinohe, Aomori 039-2586, Japan
T. Syouji
Affiliation:
Shichinohe Research Unit, National Institute of Animal Health, Shichinohe, Aomori 039-2586, Japan
T. Onodera
Affiliation:
Shichinohe Research Unit, National Institute of Animal Health, Shichinohe, Aomori 039-2586, Japan
K. Kawashima
Affiliation:
Shichinohe Research Unit, National Institute of Animal Health, Shichinohe, Aomori 039-2586, Japan
S. Inumaru
Affiliation:
National Institute of Animal Health, Tsukuba, Ibaraki 305-0856, Japan
Y. Yokomizo
Affiliation:
National Institute of Animal Health, Tsukuba, Ibaraki 305-0856, Japan
*
*Address for correspondence: International Livestock Research Institute, PO Box 30709, Nairobi, Kenya. Fax: +254 2 631499 Email: [email protected]
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Abstract

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The effects of interferon (IFN) γ on the course of infection with Strongyloides papillosus in calves were investigated. Calves (N=7 each) were inoculated with recombinant bovine IFNγ or control solution daily from day 0 to day 15 following S. papillosus infection. Treatment with IFNγ induced an increase in faecal egg output in the peak stage of infection. The IFNγ-treated animals harboured more worms, especially more immature worms, in the small intestine than control animals at necropsy on day 17, with no decreases in intestinal mucosal mast cells. Both animal groups had similar small numbers of intestinal worms at necropsy on day 26. All control animals developed peripheral blood eosinophilia on day 7, while five of seven IFNγ-treated animals did not. Serum α1-acid glycoprotein concentrations increased on day 7 in both animal groups, with higher values in control animals than in IFNγ-treated animals. Control animals mounted a predominant IgG1 response to S. papillosus from day 10, while IFNγ-treated animals did from day 22. These data suggested that IFNγ inhibited some host protective responses to S. papillosus migrating larvae, resulting in an improvement of worm survival after a period when protective responses should be activated during the early stage of infection. The effects of IFNγ on intestinal worm expulsion should be confirmed by further experiments.

Type
Review Article
Copyright
Copyright © Cambridge University Press 2002

References

Abe, T., Sugaya, H. & Yoshimura, K. (1998) Analysis of T cell populations and IL-3 mRNA expression in mesenteric lymph node cells and intestinal intra-epithelial lymphocytes in Strongyloides rath-infected mice. Journal of Helminthology 72, 18.CrossRefGoogle Scholar
Canals, A., Zarlenga, D.S., Almeria, S. & Gasbarre, L.C. (1997) Cytokine profile induced by a primary infection with Ostertagia ostertagi . Veterinary Immunology and Immunopathology 58, 6375.CrossRefGoogle ScholarPubMed
Cox, D.D. & Todd, A.C. (1962) Survey of gastrointestinal parasitism in Wisconsin dairy cattle. Journal of the American Veterinary Medical Association 141, 706709.Google ScholarPubMed
Else, K.J., Hultner, L. & Grencis, R.K. (1992) Cellular immune responses to the nematode parasite Trichuris muris. II. Differential induction of Th cell subsets in resistant versus susceptible mice. Immunology 75, 232237.Google Scholar
Else, K.J., Finkelman, F.D., Maliszewski, C.R. & Grencis, R.K. (1994) Cytokine-mediated regulation of chronic intestinal helminth infection. Journal of Experimental Medicine 179, 347351.CrossRefGoogle ScholarPubMed
Estes, D.M. (1996) Differentiation of B cells in the bovine. Role of cytokines in immunoglobulin isotype expression. Veterinary Immunology and Immunopathology 54, 6167.CrossRefGoogle ScholarPubMed
Finkelman, F.D., Madden, K.B., Cheever, A.W., Katona, I.M., Morris, S.C., Gately, M.K., Hubbard, B.R., Gause, W.C. & Urban, J.F. Jr. (1994) Effects of interleukin 12 on immune responses and host protection in mice infected with intestinal nematode parasites. Journal of Experimental Medicine 179, 15631572.CrossRefGoogle ScholarPubMed
Gasbarre, L.C., Nansen, P., Monrad, J., Grønvold, J., Steffan, P. & Henriksen, S.A. (1993) Serum anti-trichostrongyle antibody responses of first and second grazing calves. Research in Veterinary Science 54, 340344.CrossRefGoogle ScholarPubMed
Hilderson, H., De Graaf, D.C., Vercruysse, J. & Berghen, P. (1993) Characterisation of Ostertagia ostertagi antigens by the different bovine immunoglobulin isotypes. Research in Veterinary Science 55, 203208.CrossRefGoogle ScholarPubMed
Imamura, F. & Takatsu, T. (1995) Interleukin 5 and its receptor. Clinical Immunology 27 (Suppl. 16), 5863 (in Japanese).Google Scholar
Itoh, H., Tamura, K. & Motoi, Y. (1990) Serum α1-acid glycoprotein in cattle with inflammatory disease and that after operation. Journal of Veterinary Medical Science 52, 12931296.Google Scholar
Khan, A.I., Horii, Y., Tiuria, R., Sato, Y. & Nawa, Y. (1993) Mucosal mast cells and the expulsive mechanisms of mice against Strongyloides venezuelensis . International Journal for Parasitology 23, 551555.CrossRefGoogle ScholarPubMed
Korenaga, M., Hitoshi, Y., Yamaguchi, N., Sato, Y., Takatsu, K. & Tada, I. (1991) The role of interleukin-5 in protective immunity to Strongyloides venezuelensis infection in mice. Immunology 72, 502507.Google ScholarPubMed
Koyama, K. & Ito, Y. (1996) Comparative studies on immune responses to infection in susceptible B10.BR mice infected with different strains of the murine nematode parasite Trichuris muris . Parasite Immunology 18, 257263.CrossRefGoogle ScholarPubMed
Madden, K.B., Urban, J.F. Jr., Ziltener, H.J., Schrader, J.W., Finkelman, F.D. & Katona, I.M. (1991) Antibodies to IL-3 and IL-4 suppress helminth-induced intestinal mastocytosis. Journal of Immunology 147, 13871391.CrossRefGoogle ScholarPubMed
Matsuda, S., Uchikawa, R., Yamada, M. & Arizono, N. (1995) Cytokine mRNA expression profiles in rats infected with Nippostrongylus brasiliensis . Infection and Immunity 63, 46534660.CrossRefGoogle ScholarPubMed
Murrell, K.D. (1981) Protective role of immunoglobulin G in immunity to Strongyloides ratti . Journal of Parasitology 67, 167173.CrossRefGoogle ScholarPubMed
Nakamura, Y., Tsuji, N. & Taira, N. (1994) Wasting condition under normal cardiac rhythms in rabbits following Strongyloides papillosus infection. Journal of Veterinary Medical Science 56, 10051007.CrossRefGoogle ScholarPubMed
Nakamura, Y., Ooba, C. & Hirose, H. (1998) Recovery from arrhythmias in lambs infected with Strongyloides papillosus following worm elimination. Journal of Helminthology 72, 4346.CrossRefGoogle ScholarPubMed
Nakanishi, N., Nakamura, Y., Ura, S., Tsuji, N., Taira, N., Tanimura, N. & Kubo, M. (1993) Sudden death of calves by experimental infection with Strongyloides papillosus. III. Hematological, biochemical and histo-logical examinations. Veterinary Parasitology 47, 6776.CrossRefGoogle Scholar
Nwaorgu, O.C. & Connan, R.M. (1980) The migration of Strongyloides papillosus in rabbits following infection by the oral and subcutaneous routes. Journal of Helminthology 54, 223232.CrossRefGoogle ScholarPubMed
Ooba, C., Nakamura, Y. & Taira, N. (1996) Larval somatic antigens effective in inducing an IgG response and protection against Strongyloides papillosus infection. Journal of Helminthology 70, 231235.CrossRefGoogle ScholarPubMed
Ratynska-Prill, D. (1980) Immunity to strongyloidosis in experimentally infected goats II. Immunological studies. Acta Parasitologica Polonica 27, 93100.Google Scholar
Svetic, A., Madden, K.B., Zhou, X., Lu, P., Katona, I.M., Finkelman, F.D., Urban, J.F. Jr. & Gause, W.C. (1993) A primary intestinal helminthic infection rapidly induces a gut-associated elevation of Th2-associated cytokines and IL-3. Journal of Immunology 150, 34343441.CrossRefGoogle ScholarPubMed
Turner, J.H., Shalkop, W.T. & Wilson, G.I. (1960) Experimental strongyloidiasis in sheep and goats. IV. Migration of Strongyloides papillosus in lambs and accompanying pathologic changes following percutaneous infection. American Journal of Veterinary Research 21, 536546.Google ScholarPubMed
Urban, J.F. Jr., Katona, I.M., Paul, W.E. & Finkelman, F.D. (1991) Interleukin 4 is important in protective immunity to a gastrointestinal nematode infection in mice. Proceedings of the National Academy of Sciences of USA 88, 55135517.CrossRefGoogle ScholarPubMed
Urban, J.F. Jr., Madden, K.B., Cheever, A.W., Trotta, P.P., Katona, I.M. & Finkelman, F.D. (1993) IFN inhibits inflammatory responses and protective immunity in mice infected with the nematode parasite, Nippostrongylus brasiliensis . Journal of Immunology 151, 70867094.CrossRefGoogle ScholarPubMed