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Effects of luxabendazole on the tegument of Fasciola hepatica

Published online by Cambridge University Press:  05 June 2009

S.R. Stoitsova  and
L.N. Gorchilova
S.R. Stoitsova
Affiliation:
Institute of Parasitology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 25, 1113 Sofia, Bulgaria
L.N. Gorchilova
Affiliation:
Institute of Parasitology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 25, 1113 Sofia, Bulgaria
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Abstract

The effects in vivo of 5, 10, and 20 mg/kg of luxabendazole (LBZ) on the tegument of Fasciola hepatica have been examined 48 h, 7 days and 14 days post-treatment of experimentally-infected rats. As early as 48 h post-treatment, the drug is shown to provoke significant damage to the tegument. The pathological phenomena characterizing LBZ damage are blebbing of the apical plasmalemma, formation of microvillus-like projections over the free surface, swelling of the basal infolds and stimulation of autophagy. The spines are often fractured; the tegument in the vicinity of spines seems more strongly altered than that in other foci. The basal layer is often changed, from increase of electron density to lack of integrity with the apical cytoplasm. The progress of the ultrastructural damage with time is not expressed. However, cytochemical data show that at longer post-treatment intervals the surface-coat structure becomes irregular and patches of ruthenium red positive material of variable thickness are focally accumulated. Only a slight dose-effect is noted 48 h after LBZ application when the alterations provoked by 5 mg/kg are less evident than those by 10 and 20 mg/kg.

Type
Research Papers
Information
Journal of Helminthology , Volume 68 , Issue 1 , March 1994 , pp. 73 - 80
Copyright
Copyright © Cambridge University Press 1994

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