Hostname: page-component-848d4c4894-v5vhk Total loading time: 0 Render date: 2024-07-07T16:54:28.516Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of free and liposomized praziquantel on worm burden and antibody response in mice infected with Mesocestoides corti tetrathyridia

Published online by Cambridge University Press:  05 June 2009

G. Hrčkova  and
S. Velebny
G. Hrčkova
Affiliation:
Parasitological Institute of the Slovak Academy of Sciences, Hlinkova 3, 040 01 Košice, Slovak Republic
S. Velebny
Affiliation:
Parasitological Institute of the Slovak Academy of Sciences, Hlinkova 3, 040 01 Košice, Slovak Republic
Get access Rights & Permissions [Opens in a new window]

Abstract

The parasite burden in the liver and peritoneal cavity and antibody levels directed to whole worm extract, have been monitored in serum from ICR-strain mice, infected orally with 55 tetrathyridia of Mesocestoides corti (Cestoda, Cyclophyllidea). The subcurative doses (3x or 6x) of praziquantel (PZQ) (10 mg.kg−1 body weight) were administered to mice from day 14 post infection (p.i.) in two drug formulations: as PZQ suspended in Dorfman vehicle, or as PZQ incorporated in liposomes (lip.PZQ). The appearance of antibodies was time-dependent and correlated with the rate of reduction in numbers of tetrathyridia. PZQ in three and six doses caused the highest fall of parasite numbers in the liver on day 1 post therapy (p.t.). In the peritoneal cavity, a similar reduction in worm burden occurred but only after six doses of the drug. The worm count in the peritoneal cavity from groups of mice injected with lip.PZQ decreased most markedly on day 7 p.t., in the group treated with six doses of the drug. In the liver, the highest larvicidal effect, compared with the controls, was observed 6 days later (i.e. day 13 p.t.), following three doses of lip.PZQ. In all treated groups, two peak values of antitetrathyridial antibody levels were detected between days 1 and 13 p.t. (i.e. days 17 to 29 p.i.), after which there was a gradual but continuous increase in antibody titre. After treatment with six doses of both drug formulations (PZQ and lip.PZQ), the occurrence of the first maximum and minimum of antibody serum levels was delayed 6 days, compared to those groups treated with three doses of the drugs. Empty liposomes had some immunomodulatory effect on the antibody response, with significant decrease in antibody titres after three doses over the experimental period, but not after six doses.

Type
Research Papers
Information
Journal of Helminthology , Volume 69 , Issue 3 , September 1995 , pp. 213 - 221
Copyright
Copyright © Cambridge University Press 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abraham, K.M. & Teale, J.M. (1987) the contribution of parasite specific T cells to isotype restriction in Mesocestoides corti infected mice. Journal of Immunology 139, 2530–2537.CrossRefGoogle ScholarPubMed
Akvarieh, M., Besner, J.G., Galal, A. & Tawashi, R. (1992) Liposomal delivery system for the targeting and control release of praziquantel. Drug Development and Industrial Pharmacy 18, 303–317.CrossRefGoogle Scholar
Allison, A.C. (1979) Mode of action of immunological adjuvants. Journal of the Reticuloendothelial Society 26, 619–630.Google ScholarPubMed
Andrews, P. (1981) A summary of the efficacy of praziquantel against schistosomes in animal experiments and notes on its mode of action. Arzneimittel Forschung 31,538–541.Google ScholarPubMed
Bangham, A.D. (1968) Membrane models with phospholipids. Progress in Biophysics and Molecular Biology 18, 29–95.CrossRefGoogle ScholarPubMed
Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 1–2, 248–254.CrossRefGoogle Scholar
Brindley, P.J. & Sher, A. (1987) The chemotherapeutic effect of praziquantel against Schistosoma mansoni is dependent on host antibody response. Journal of Immunology 136, 215–220.CrossRefGoogle Scholar
Cha, Y.N. & Edwards, R. (1976) Effect of Schistosoma mansoni infection on the hepatic drug-metabolizing capacity of mice. Journal of Pharmacology and Experimental Therapy 199, 432–440.Google ScholarPubMed
Chavasse, C.J., Brown, M.C. & Bell, D.R. (1979) Schistosoma mansoni: Activity responses in vitro to praziquantel. Zeitschrift für Parasitenkunde 58, 169–174.CrossRefGoogle ScholarPubMed
Doenhoff, M., Sabah, A.A., Fletcher, C., Webbe, G. & Bain, J. (1987) Evidence for an immune-dependent action of praziquantel of Schistosoma mansoni in mice. Transactions of the Royal Society for Tropical Medicine and Hygiene 81, 947–951.CrossRefGoogle ScholarPubMed
Dorfman, I.R. (1962) Methods in hormone research. pp. 709–711. New York, London, Academic Press.Google Scholar
El-Ridy, M.S., Akbarieh, M., Besner, J.G., Kassem, M., Sharkawi, M. & Tawashi, R. (1989) Chemoprophylaxis of schistosomiasis using liposome-encapsulated tartar emetic. International Journal of Pharmaceutics 56, 23–27.CrossRefGoogle Scholar
Etges, F.J. (1991) The proliferative tetrathyridium of Mesocestoides vogae sp.n. (Cestoda). Journal of the Helminthological Society of Washington 58, 181–185.Google Scholar
Fallon, P.G., Cooper, R.O., Probert, A.J. & Doenhoff, M.J. (1992) Immune-dependent chemotherapy of schistosomiasis. Parasitology 105, S41–S48.CrossRefGoogle ScholarPubMed
Flisser, A., Gonzalez, D., Plancarte, A., Ostrovsky, P., Montero, R., Stephano, A. & Correa, D. (1990) Praziquantel treatment of brain and muscle porcine Taenia solium cysticercosis. 2. Immunological and cytogenetic studies. Parasitological Research 76, 640–642.CrossRefGoogle ScholarPubMed
Gönnert, R. & Andrews, P. (1977) Praziquantel, a new broadspectrum antischistosomal agent. Zeitschrift für Parasitenkunde 52, 129–150.CrossRefGoogle Scholar
Gregoriadis, G. (1973) Drug entrapment in liposomes. FEBS Letters 36, 292–296.CrossRefGoogle ScholarPubMed
Harder, A., Andrews, P. & Thomas, H. (1987) Praziquantel: mode of action. Biochemical Society Transactions 15, 68–70.CrossRefGoogle ScholarPubMed
Harnett, W. & Kusel, J.R. (1986) Increased exposure of parasite antigens at the surface of adult male Schistosoma mansoni exposed to praziquantel in vitro. Parasitology 93, 401–405.CrossRefGoogle ScholarPubMed
Hirano, K. & Hunt, A.C. (1985) Lymphatic transport of liposome-encapsulated agents: Effects of liposome size following intraperitoneal administration. Journal of Pharmaceutical Sciences 74, 915–921.CrossRefGoogle ScholarPubMed
Horton, R.J. (1989) Chemotherapy of Echinococcus infection in man with albendazole. Transactions of the Royal Society for Tropical Medicine and Hygiene 83, 97–102.CrossRefGoogle ScholarPubMed
Hrčkova, G. & Velebny, S. (1994) Studies on the effects of free and liposomised albendazole on infection of Mesocestoides corti tetrathyridia (Cestoda: Cyclophyllidea) in laboratory mice. Comparative Biochemistry and Physiology 107C, 71–77.Google Scholar
Mančal, P. (1983) Methods in enzymatic analysis. Bulletin of the Institute of Sera and Vaccines (USOL) 83, Prague. (In Czech)Google Scholar
Mitchell, G.F., Marchalonis, J.J., Smith, P.M., Nicholas, W.L. & Warner, N.L. (1977) Studies on immune responses to larval cestodes in mice. Immunoglobulins associated with the larvae of Mesocestoides corti. Australian Journal of Experimental Biology and Medical Science 55, 187–211.CrossRefGoogle ScholarPubMed
Mitchell, G.F. & Handman, E. (1977) Studies on immune responses to larval cestodes in mice: A simple mechanism of non-specific immunosuppression in Mesocestoides cortiinfected mice. Australian Journal of Experimental Biology and Medical Science 55, 615–622.CrossRefGoogle ScholarPubMed
Novak, M. (1977) Efficacy of a new cesticide, praziquantel, against larval Mesocestoides corti and Taenia crassiceps in mice. Journal of Parasitology 63, 949–950.CrossRefGoogle Scholar
Papahadjopoulos, D. (1993) Optimal liposomal drug action: from serependity to targeting. pp. 1–15 in Gregoriadis, G. (Ed.) Liposome technology. vol. 3. Interactions of liposomes with biological milieu. 2nd edition, Boca Raton, CRC Press.Google Scholar
Putter, J. (1979) Extramicrosomal drug metabolism. European Journal of Metabolism and Pharmacokinetics 4, 1–7.CrossRefGoogle ScholarPubMed
Rumjanek, F.D. & Simpson, A.J.G. (1980) The incorporation and utilization of radiolabelled lipids by adultSchistosoma mansoni in vitro. Molecular Biochemistry and Parasitology 1, 31–44.CrossRefGoogle ScholarPubMed
Schantz, P.M., Bossche, H.van den & Eckert, J. (1982) Chemotherapy for larval echinococcosis in animals and humans: report of a workshop. Zeitschrift für Parasitenkunde 67, 5–26.CrossRefGoogle ScholarPubMed
Shaw, M.K. & Erasmus, D.A. (1983a) Schistosoma mansoni: the effects of subcurative dose of praziquantel on the ultrastructure of worms in vivo. Zeitschrift für Parasitenkunde 69, 73–90.CrossRefGoogle ScholarPubMed
Shaw, M.K. & Erasmus, D.A. (1983b) Schistosoma mansoni: Dose related tegumental surface changes after in vivo treatment with praziquantel. Zeitschrift für Parasitenkunde 69, 643–653.CrossRefGoogle ScholarPubMed
Smith, W.G. & Butchko, G.M. (1986) Regulation of in vivo IgE biosynthesis in mice with complete Freund's adjuvant. International Archives of Allergy and Applied Immunology 79, 337–343.CrossRefGoogle ScholarPubMed
Sogandares-Bernal, F. & Voge, M. (1978) Immunoglobulins on the surfaces of tetrathyridia of Mesocestoides corti Hoeppli 1925 (Cestoda), from laboratory infections of ICR mice. Journal of Parasitology 64, 620–624.CrossRefGoogle ScholarPubMed
Specht, D. & Widmer, E.A. (1972) Response of mouse liver to infection with tetrathyridia of Mesocestoides (Cestoda). Journal of Parasitology 58, 431–437.CrossRefGoogle ScholarPubMed
Steiner, K., Garbe, A., Diekmann, H. & Nowak, H. (1976) The fate of praziquantel in the organism I. Pharmacokinetics in animals. European Journal of Drug Metabolism and Pharmacokinetics 1, 85–95.CrossRefGoogle Scholar
Steiner, K. & Garbe, A. (1976) The fate of praziquantel in the organism.II. Distribution in rats. European Journal of Drug Metabolism and Pharmacokinetics 1, 97–106.CrossRefGoogle Scholar
Strazhenskykh, A.N., Molokova, O.A., Chabanov, M.K., Butchkov, V.G. & Yarotsky, L.S. (1990) Comparative efficacy of pure and liposomal praziquantel in experimental opisthorchiasis. Medicinskaja Parasitologia 4, 39–41. (In Russian)Google Scholar
Thomas, H. & Gönnert, R. (1977) The efficacy of praziquantel against cestodes in animals. Zeitschrift für Parasitenkunde 52, 117–127.CrossRefGoogle ScholarPubMed
Thomas, H. & Gönnert, R. (1978) The efficacy of praziquantel against experimental cysticercosis and hydatidosis. Zeitschrift für Parasitenkunde 55, 165–179.CrossRefGoogle ScholarPubMed
Thomas, H., Gönnert, R., Pohlke, R. & Seubert, J. (1975) A new compound against adult tapeworms. 7th International conference on Pathophysiology of Parasitic Infections, Thessaloniki, abstract No. 51.Google Scholar
WHO (1984) Guidelines for surveillance, prevention and control of echinococcosis/hydatatidosis. 2nd edn.Geneva, World Health Organization.Google Scholar