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Effects of Bothriocephalus acheilognathi on the polarization response of pronephric leucocytes of carp, Cyprinus carpio

Published online by Cambridge University Press:  12 April 2024

P. Nie  and
D. Hoole
P. Nie
Affiliation:
State Key Laboratory of Freshwater Ecology and Biotechnology, and Laboratory of Fish Diseases, Institute of Hydrobiology Chinese Academy of Sciences, Wuhan, Hubei 430072, People's Republic of China
D. Hoole*
Affiliation:
Parasitology Research Laboratory, Centre for Applied Entomology and Parasitology, Huxley Building, School of Life Sciences, Keele University, Keele, Staffordshire, ST5 5BG, UK
*
*Author for correspondence Fax: +44(0) 1782 583516 E-mail: [email protected]
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Abstract

An in vitro assay was used to examine the effect of Bothriocephalus acheilognathi Yamaguti, 1934 (Cestoda: Pseudophyllidea) on the polarization response of pronephric leucocytes of carp, Cyprinus carpio. Leucocytes, isolated from naive, naturally-infected fish and carp injected intraperitoneally with cestode extracts, were exposed to parasite extracts (protein concentrations 0–10.0 μg ml-1), for up to 24 h in the presence or absence of carp serum. In general, polarization responses of the pronephric leucocytes, primarily neutrophils and eosinophils, increased with incubation time although there was no significant difference in the response induced by the different protein concentrations. Differences in the polarization response were, however, observed in naive, naturally infected and injected fish and the cells responded differently in the presence and absence of carp serum. In the absence of carp serum the polarization response of pronephric leucocytes in vitro was significantly reduced with cells obtained from injected and naturally infected fish compared with those obtained from naive carp. This suppression of leucocyte migration was however reduced by the addition of carp serum to the in vitro system. The role of this interaction between the possible suppression of polarization induced by the parasite and stimulation by serum is discussed.

Type
Research Article
Information
Journal of Helminthology , Volume 74 , Issue 3 , September 2000 , pp. 253 - 257
Copyright
Copyright © Cambridge University Press 2000

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