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Effect of Echinococcus multilocularis on the origin of acetyl-CoA entering the tricarboxylic acid cycle in host liver

Published online by Cambridge University Press:  12 April 2024

C. Kepron ,
M. Novak  and
B.J. Blackburn
C. Kepron
Affiliation:
Department of Biology, University of Winnipeg, Winnipeg, Manitoba, Canada R3B 2E9
M. Novak*
Affiliation:
Department of Biology, University of Winnipeg, Winnipeg, Manitoba, Canada R3B 2E9
B.J. Blackburn
Affiliation:
Department of Chemistry, University of Winnipeg, Winnipeg, Manitoba, Canada R3B 2E9
*
*Author for correspondence Fax: 204 774 4134 Email: [email protected]
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Abstract

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Carbon-13 nuclear magnetic resonance (NMR) spectroscopy was employed to investigate alterations in hepatic carbohydrate metabolism in Meriones unguiculatus infected with Echinococcus multilocularis. Following portal vein injections of an equimolar mixture of ]#x005B;1,2-13C2]acetate and [3-13C]lactate, perchloric acid extracts of the livers were prepared and NMR spectra obtained. Isotopomer analysis using glutamate resonances in these spectra showed that the relative contributions of endogenous and exogenous substrates to the acetyl-CoA entering the tricarboxylic acid cycle differed significantly between infected and control groups. The mole fraction of acetyl-CoA that was derived from endogenous, unlabelled sources (FU) was 0.50±0.10 in controls compared to 0.34±0.04 in infected animals. However, the fraction of acetyl-CoA derived from [3-13C]lactate (FLL) was larger in livers of infected animals than those from controls with values of 0.27±0.04 and 0.18±0.04, respectively. Similarly, the fraction of acetyl-CoA derived from [1,2-13C2]acetate (FLA) was larger in livers of infected animals compared to those in controls; the fractions were 0.38±0.01 and 0.32±0.07, respectively. The ratio of FLA:FLL was significantly smaller in the infected group with a value of 1.42±0.18 compared to 1.74±0.09 for the controls. These results indicate that alveolar hydatid disease has a pronounced effect on the partitioning of substrates within the pathways of carbohydrate metabolism in the host liver.

Type
Review Article
Information
Journal of Helminthology , Volume 76 , Issue 1 , March 2002 , pp. 31 - 36
Copyright
Copyright © Cambridge University Press 2002

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