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Effect of antibiotics on protein synthesis in filarial parasites

Published online by Cambridge University Press:  05 June 2009

J. H. Wani  and
V. M. L. Srivastava
J. H. Wani
Affiliation:
Division of Biochemistry, Central Drug Research Institute, Lucknow 226001, U.P., India
V. M. L. Srivastava
Affiliation:
Division of Biochemistry, Central Drug Research Institute, Lucknow 226001, U.P., India
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Abstract

Acanthocheilonema viteae, Litomosoides carinii and Setaria cervi were found to actively synthesize proteins in vitro. Different centrifugation fractions and their TCA-precipitable fractions were assessed for the distribution of newly synthesized proteins. Penicillin and streptomycin inhibited the process in A. viteae. The synthesis in S. cervi was susceptible to puromycin, chloramphenicol, cycloheximide, neomycin and polymyxin B. The process in L. carinii was strongly blocked by puromycin while chloramphenicol had no significant effect.

Keywords

Acanthocheilonema viteaeLitomosoides cariniiSetaria cerviprotein synthesisantibioticsNematodafilariidschemotherapy
Type
Research Papers
Information
Journal of Helminthology , Volume 67 , Issue 4 , December 1993 , pp. 265 - 270
Copyright
Copyright © Cambridge University Press 1993

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References

REFERENCES

Agosin, M. & Naquira, C. (1978) Translation of Taenia crassiceps mRNA in cell-free heterologous systems. Comparative Biochemistry and Physiology, 60B, 183–187.Google Scholar
Agosin, M. & Repetto, Y. (1967) Studies on the metabolism of Echinococcus granulosus. IX. Protein synthesis in scoleces. Experimental Parasitology, 21, 195–208.Google Scholar
Barrett, J. (1981) In: Biochemistry of Parasitic Helminths. MacMillan Publishers, London.CrossRefGoogle Scholar
Barrett, J. (1983) Biochemistry of filarial worms. Helminthological Abstracts, 52, 1–18.Google Scholar
Barrett, J. (1984) The anaerobic end-products of helminths. Parasitology, 88, 179–198.Google Scholar
Chappell, L.H. & Read, C.P. (1973) Studies on the free pool of amino acids of the cestode Hymenolepis diminuta. Parasitology, 67, 289–305.CrossRefGoogle ScholarPubMed
Culbreth, K.L., Esch, G.W. & Kuhn, R. E. (1972) Growth and development of larval Taenia crassiceps (Cestoda). Experimental Parasitology, 32, 272–281.Google Scholar
Gordon, R. & Webster, J. M. (1972) Nutritional requirements for protein synthesis during parasitic development of the entomophilic nematode Mermis nigrescens. Parasitology, 64, 161–172.Google Scholar
Harris, B.G. & Read, C.P. (1969) Factors affecting protein synthesis in H. diminuta (Cestoda). Comparative Biochemistry and Physiology, 28,645–654.Google Scholar
Jeffs, S.A. & Arme, C. (1984) Hymenolepis diminuta: Protein synthesis in cysticercoids. Parasitology, 88, 351–357.CrossRefGoogle Scholar
Kilejian, A. (1966) Permeation of L-proline in the cestode, Hymenolepis diminuta. Journal of Parasitology, 52, 1108–1115.CrossRefGoogle ScholarPubMed
Kohler, P. (1985) The strategies of energy conservation in helminths. Molecular and Biochemical Parasitology, 17, 1–18.CrossRefGoogle ScholarPubMed
Naquira, C., Paulin, J. & Agosin, M. (1977) Taenia crassiceps: Protein synthesis in larvae. Experimental Parasitology, 41, 359–369.CrossRefGoogle ScholarPubMed
Parker, R.D. Jr & Mcinnis, A.J. (1977) H. diminuta: Isolation, purification and reconstitution in vitro of a cell free system for protein synthesis. Experimental Parasitology, 41, 2–16.Google Scholar
Read, C. P., Rothman, A.H. & Simmons, J.E. Jr (1963) Studies on membrane transport with special reference to parasite-host integration. Annals of the New York Academy of Sciences, 113, 154–205.Google Scholar
Roy, T.K. & Srivastava, V.M.L. (1981) Cotugnia digonopora: Transport of leucine. Experimental Parasitology, 51, 21–27.Google Scholar
Tlelens, A.G.M. & Van Den Bergh, S.G. (1985) The (an)aerobic energy metabolism of parasitic helminths. Molecular Physiology, 8, 359–369.Google Scholar
Wani, J.H. (1991) Calcium influx into the mitochondria isolated from cuticle of Setaria cervi: Effect of antibiotics. Indian Journal of Parasitology, 16, 51–53.Google Scholar
Wani, J.H., Katiyar, J.C. & Srivastava, V.M.L. (1992) Calcium influx into the mitochondria of Hymenolepis diminuta. Medical Science Research, 20, 925–928.Google Scholar