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Echinococcus granulosus: observations on strobilar development in in vitro monophasic culture

Published online by Cambridge University Press:  05 June 2009

F. Ponce Gordo  and
C. Cuesta Bandera
F. Ponce Gordo
Affiliation:
Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense de Madrid, Ciudad Universitaria, s/n 28040 Madrid, Spain
C. Cuesta Bandera
Affiliation:
Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense de Madrid, Ciudad Universitaria, s/n 28040 Madrid, Spain
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Abstract

The in vitro cultivation technique of Echinococcus granulosus protoscoleces usually states the necessity of a biphasic medium with a solid protein substrate for strobilar development to take place; otherwise, in a monophasic medium, protoscoleces follow a vesicular development. However, in some monocphasic cultures, the development of several strobilate individuals (in different quantities and stages of development, depending on the culture) were observed. The only known diference form cultures made previously and snice, where the development was vesicular, was the batch of foetal calf serum used in the constitution of the liquid medium, and this is presumed to be the cause of this unexpected strobilar development.

Type
Research Note
Information
Journal of Helminthology , Volume 69 , Issue 2 , June 1995 , pp. 173 - 175
Copyright
Copyright © Cambridge University Press 1995

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