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The distribution of neuroactive substances within the cercaria of Sanguinicola inermis

Published online by Cambridge University Press:  05 June 2009

D.F. McMichael-Phillips ,
J.W. Lewis  and
M.C. Thorndyke
D.F. McMichael-Phillips
Affiliation:
School of Biological Sciences, Royal Holloway, University of London Egham, Surrey, TW20 OEX, UK
J.W. Lewis
Affiliation:
School of Biological Sciences, Royal Holloway, University of London Egham, Surrey, TW20 OEX, UK
M.C. Thorndyke*
Affiliation:
School of Biological Sciences, Royal Holloway, University of London Egham, Surrey, TW20 OEX, UK
*
2Author for correspondence.
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Abstract

The serotoninergic and peptidergic components of the nervous system of the cercaria of Sanguinicola inermis (Digenea: Sanguinicolidae) were examined using whole-mount immunocytochemistry and a plan of the nervous system has been described. Antibodies to serotonin (5-hydroxytryptamine, 5-HT) and the neuropeptides, FMRFamide, GFNSALMFamide (S1) and SGPYSFNSGLTFamide (S2) were used in the study. Immunoreactivity (IR) was demonstrated to all but the S2 antisera and showed a similar fundamental distribution. IR was found in paired cerebral ganglia located anteriorly within the body and connected by a cerebral commissure. From the ganglia paired ventral and dorsal longitudinal nerve cords extend anteriorly into the cephalic organ and into the body. There is no apparent connection with the tail. Several transverse commissures connect the longitudinal nerve cords throughout the body and several associated cell bodies have been located. A double-stranded dorsal and ventral longitudinal nerve cord extends the length of the tail and six cell bodies are associated with these cords, uniquely demonstrating either FMRFamide and S1, or 5-HT-like IR. Only 5-HT-like IR was found to extend into the posterior tail furcae and there appears to be a lack of any peripheral tegumental innervation. Double-labelling experiments suggest that the serotoninergic and peptidergic components of the cercarial nervous system are distinct.

Type
Research Papers
Information
Journal of Helminthology , Volume 70 , Issue 4 , December 1996 , pp. 309 - 317
Copyright
Copyright © Cambridge University Press 1996

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