Hostname: page-component-586b7cd67f-g8jcs Total loading time: 0 Render date: 2024-11-30T05:11:39.108Z Has data issue: false hasContentIssue false
  • English
  • Français

Diagnostic potential of Fasciola gigantica-derived 14.5 kDa fatty acid binding protein in the immunodiagnosis of bubaline fascioliasis

Published online by Cambridge University Press:  13 March 2012

G. Allam ,
I.R. Bauomy ,
Z.M. Hemyeda ,
T.M. Diab  and
T.F. Sakran
G. Allam*
Affiliation:
Department of Zoology, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt Department of Microbiology, College of Medicine, Taif University, Taif, Saudi Arabia
I.R. Bauomy
Affiliation:
Department of Parasitology, Theodor Bilharz Research Institute, Giza, Egypt
Z.M. Hemyeda
Affiliation:
Department of Zoology, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt
T.M. Diab
Affiliation:
Department of Parasitology, Theodor Bilharz Research Institute, Giza, Egypt
T.F. Sakran
Affiliation:
Department of Zoology, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt
*
*Fax: +966 2 7250528 E-mail: [email protected]; [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

The 14.5 kDa fatty acid binding protein (FABP) was isolated from the crude extract of adult Fasciola gigantica worms. Polyclonal anti-FABP IgG was generated in rabbits immunized with prepared FABP antigen. Sandwich enzyme-linked immunosorbent assay (ELISA) was applied to detect coproantigen in stools and circulating Fasciola antigen (CA) in sera of 126 water buffaloes by using purified and horseradish peroxidase (HRP)-conjugated anti-FABP IgG. Sandwich ELISA sensitivity was 96.97% and 94.95%; while specificity was 94.12% and 82.35% for coproantigen and CA detection, respectively. However, sensitivity and specificity of the Kato–Katz technique was 73.74% and 100%, respectively. The diagnostic efficacy of sandwich ELISA was 96.55% and 93.1% for coproantigen and CA detection, respectively. In contrast, the diagnostic efficacy of the Kato–Katz technique was 77.59%. In conclusion, these results demonstrate that the purified 14.5 kDa FABP provides a more suitable antigen for immunodiagnosis of early and current bubaline fascioliasis by using sandwich ELISA.

Type
Research Papers
Information
Journal of Helminthology , Volume 87 , Issue 2 , June 2013 , pp. 147 - 153
Copyright
Copyright © Cambridge University Press 2012 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abdel-Rahman, S., O'Reilly, K.L. & Malone, J.B. (1999) Biochemical characterization and localization of Fasciola hepatica 26–28 kDa diagnostic coproantigens. Parasite Immunology 21, 279–286.CrossRefGoogle Scholar
Allam, G., Bauomy, I.R., Hemyeda, Z.M. & Sakran, T.F. (2011) Evaluation of a 14.5 kDa-Fasciola gigantica fatty acid binding protein as a diagnostic antigen for human fascioliasis. Parasitology Research doi 10.1007/s00436-011-2711-y.Google ScholarPubMed
Anderson, N., Luong, T.T., Vo, G.N., Bui, L.K., Smooker, M.P. & Spithill, W.T. (1999) The sensitivity and specificity of two methods for detecting Fasciola infections in cattle. Veterinary Parasitology 83, 15–24.CrossRefGoogle ScholarPubMed
Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254.CrossRefGoogle ScholarPubMed
Carnevale, S., Rodriguez, M.I., Santillan, G., Labbe, J.H., Cabrera, M.G., Bellegarde, E.J., Velasquez, J.N., Trjoveic, J.J.E. & Guernera, E.A. (2001) Immunodiagnosis of human fascioliosis by an Enzyme-Linked Immunosorbent Assay (ELISA) and a micro-ELISA. Clinical and Diagnostic Laboratory Immunology 8, 174–177.CrossRefGoogle Scholar
Engels, D., Nathimana, S., De Vias, S.J. & Gryseels, B. (1997) Variation in weight of stool samples prepared by the Kato–Katz method and its implications. Tropical Medicine and International Health 2, 265–271.CrossRefGoogle ScholarPubMed
Espino, A.M. & Finlay, C.M. (1994) Sandwich enzyme-liked immunosorbent assay for detection of excretory-secretory antigens in humans with fascioliasis. Journal of Clinical Microbiology 32, 190–193.CrossRefGoogle Scholar
Espino, A.M., Marcet, R. & Finlay, C.M. (1990) Detection of circulating excretory antigens in human fascioliasis by sandwich enzyme-linked immunosorbent assay. Journal of Clinical Microbiology 28, 2637–2640.CrossRefGoogle ScholarPubMed
Espino, A.M., Millan, J.C. & Finlay, C.M. (1992) Detection of antibodies and circulating excretory secretory antigens for assessing cure of patients with fascioliasis. Transactions of the Royal Society of Tropical Medicine and Hygiene 86, 649–654.CrossRefGoogle ScholarPubMed
Espino, A.M., Diaz, A., Perez, A. & Finlay, C.M. (1998) Dynamics of antigenemia and coproantigens during a human Fasciola hepatica outbreak. Journal of Clinical Microbiology 36, 2723–2726.CrossRefGoogle ScholarPubMed
Espinoza, J.R., Timteo, O. & Herrera-Velit, P. (2005) Fas2-ELISA in the detection of human infection by Fasciola hepatica. Journal of Helminthology 79, 235–240.CrossRefGoogle ScholarPubMed
Estuningsih, E.S., Smooker, P.M., Wiedosari, E., Widjajanti, S., Vaiano, A., Partotomo, S. & Spithill, T.W. (1997) Evaluation of antigens of Fasciola gigantica as vaccine against tropical fascioliasis. International Journal of Parasitology 11, 1419–1428.CrossRefGoogle Scholar
Fagbemi, B.O., Obarisiagbon, I.O. & Mbuh, J.V. (1995) Detection of circulating antigen in sera of Fasciola gigantica infected cattle with antibodies reactive with a Fasciola-specific 88-kDa antigen. Veterinary Parasitology 58, 235–246.CrossRefGoogle ScholarPubMed
Fagbemi, B.O., Aderibigbe, O.A. & Guobadia, E.E. (1997) The use of monoclonal antibody for the immunodiagnosis of Fasciola gigantica infection in cattle. Veterinary Parasitology 69, 230–240.CrossRefGoogle ScholarPubMed
Feldmeier, H. & Poggensee, G. (1993) Diagnostic techniques in schistosomiasis control: a review. Acta Tropica 52, 205–220.CrossRefGoogle ScholarPubMed
Guobadia, E.E. & Fagbemi, B.O. (1997) The isolation of F. gigantica-specific antigens and their use in the serodiagnosis of fascioliasis in sheep by the detection of circulating antigens. Veterinary Parasitology 68, 269–282.CrossRefGoogle ScholarPubMed
Hassan, A.A., El-Bahy, M.M., Abou-Zinadah, N.Y. & Shalaby, H.A. (2008) The diagnostic efficacy of Fasciola gigantica coproantigen in naturally infected cattle and buffaloes. Journal of the Egyptian Society of Parasitology 38, 115–130.Google ScholarPubMed
Hillyer, G.V. (1988) Fascioliasis and fasciolopsiasis. pp. 856–862in Baloes, A., Hausler, W.J., Ohashi, M. & Turano, A. (Eds) Laboratory diagnosis of infectious diseases principles and practice. Berlin, Springer-Verlag.CrossRefGoogle Scholar
Hillyer, G.V. (2005) Fasciola antigens as vaccines against fascioliasis and schistosomiasis. Journal of Helminthology 79, 241–247.CrossRefGoogle ScholarPubMed
Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 277, 680–685.CrossRefGoogle Scholar
McKinney, M.M. & Parkinson, A. (1987) A simple, non-chromatographic procedure to purify immunoglobulins from serum and ascites fluid. Journal of Immunological Methods 96, 271–278.CrossRefGoogle ScholarPubMed
Mezo, M., González-Warleta, M. & Ubeira, F.M. (2007) The use of MM3 monoclonal antibodies for the early immunodiagnosis of ovine fascioliasis. Journal of Parasitology 93, 65–72.CrossRefGoogle ScholarPubMed
Moustafa, N.E., Hegab, M.H. & Hassan, M.M. (1998) Role of ELISA in early detection of Fasciola copro-antigens in experimentally infected animals. Journal of the Egyptian Society of Parasitology 28, 379–387.Google ScholarPubMed
Nambi, P.A., Yadav, S.C., Raina, O.K., Sriveny, D. & Saini, M. (2005) Vaccination of buffaloes with Fasciola gigantica recombinant fatty acid binding protein. Parasitology Research 97, 129–135.CrossRefGoogle ScholarPubMed
Nowotny, A. (1979) Basic exercises in immunochemistry. pp. 7–20. New York, Springer Verlag.CrossRefGoogle Scholar
Ockner, R.K. (1990) Historic overviews of the studies on fatty acid binding proteins. Molecular and Cellular Biochemistry 98, 3–9.CrossRefGoogle ScholarPubMed
O'Neill, S.M., Parkinson, M., Strauss, W., Angles, R. & Dalton, J.P. (1998) Immunodiagnosis of Fasciola hepatica infection (fascioliasis) in human population in the Bolivian Altiplano using purified cathepsin L cysteine proteinase. American Journal of Tropical Medicine and Hygiene 58, 417–423.CrossRefGoogle ScholarPubMed
Rabia, I., Salah, F., Neamat, M. & Raafat, A. (2007) Evaluation of different antigens extracted from Fasciola gigantica for effective specific diagnosis of fascioliasis. New Egyptian Journal of Medicine 36, 40–47.Google Scholar
Raina, O.K., Yadav, S.C., Sriveny, D. & Gupta, S.C. (2006) Immuno-diagnosis of bubaline fascioliasis with Fasciola gigantica cathepsin-L and recombinant cathepsin L 1-D proteases. Acta Tropica 98, 145–151.CrossRefGoogle ScholarPubMed
Ramajo, V., Oleaga, A., Casanueva, P., Hillyer, G.V. & Muro, A. (2001) Vaccination of sheep against Fasciola hepatica with homologous fatty acid binding proteins. Veterinary Parasitology 97, 35–46.CrossRefGoogle ScholarPubMed
Sheehan, D. & FitzGerald, R.F. (1996) Ion-exchange chromatography. Methods in Molecular Biology 59, 145–150.Google ScholarPubMed
Shehab, A.Y., Hassan, E.M., Basha, L.M., Omar, E.A., Helmy, M.H., El-Morshedy, H.N. & Farag, H.F. (1999) Detection of circulating E/S antigens in the sera of patients with fascioliasis by IELISA: a tool of serodiagnosis and assessment of cure. Tropical Medicine and International Health 4, 686–690.CrossRefGoogle Scholar
Sirisriro, A., Grams, R., Vichasri-Grams, S., Ardseungneon, P., Pankao, V., Meepool, A., Chaithirayanon, K., Viyanant, V., Tan-Ariya, P., Upatham, E.S. & Sobhon, P. (2002) Production and characterization of a monoclonal antibody against recombinant fatty acid binding protein of Fasciola gigantica. Veterinary Parasitology 105, 119–129.CrossRefGoogle ScholarPubMed
Snedecor, G.W. & Cochran, W.G. (1981) Statistical methods. 8th edn. 83 pp. Iowa, USA, The Iowa State University Press.Google Scholar
Tendler, M., Brito, C.A., Vilar, M.M., Serra-Freire, N., Diogo, C.M., Almeida, M.S., Delbem, A.C., De Silva, J.F., Sanivo, W., Garratt, R.C. & Simpson, A.J. (1996) A Schistosoma mansoni fatty acid binding protein Sm14 is the potential basis of dual purpose anti-helminth vaccine. Proceedings of the National Academy of Sciences of the United States of America 93, 269–273.CrossRefGoogle ScholarPubMed
Tijssen, P. & Kurstak, P. (1984) Highly efficient and simple methods for the preparation of peroxidase and active peroxidase-antibody conjugate for enzyme immunoassays. Analytical Biochemistry 136, 451–457.CrossRefGoogle ScholarPubMed
van Nieuwenhoven, F.A., Vork, M.M., Surtel, D.A., Kleine, A.H., van der Vusse, G.J. & Glatz, J.F. (1991) High-yield two-step chromatographic procedure for purification of fatty acid-binding protein from human heart. Journal of Chromatography 570, 173–179.CrossRefGoogle ScholarPubMed
Youssef, F.G., Mansour, N.S. & Aziz, A.G. (1991) Early diagnosis of human fascioliasis by the detection of copro-antigens using counterimmunoelectrophoresis. Transactions of the Royal Society of Tropical Medicine and Hygiene 85, 383–384.CrossRefGoogle ScholarPubMed
Zane, H.D. (2001) Laboratory safety and test quality assurance. pp. 193–207in Immunology: Theoretical and practical concepts in laboratory medicine. Philadelphia, Saunders WB Company.Google Scholar