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Diagnostic Mr31/32 000 Schistosoma mansoni proteins (Sm31/32): Reaction with sera from Sudanese patients infected with S. mansoni or S. haematobium

Published online by Cambridge University Press:  05 June 2009

Mohamed A. Idris  and
Andreas Ruppel
Mohamed A. Idris
Affiliation:
Institute for Tropical Hygiene, University of Heidelberg, Federal Republic of Germany
Andreas Ruppel*
Affiliation:
Institute for Tropical Hygiene, University of Heidelberg, Federal Republic of Germany
*
†Dr. A. Ruppel, Institut für Tropenhygiene, Im Neuenheimer Feld 324, D–6900 Heidelberg, F.R.G.
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Abstract

Sera of Sudanese patients with active infections of Schistosoma mansoni or S. haematobium were tested in immunoblots for their reactivity with Mr31/32 000 proteins of adult S. mansoni (Sm31/32). All sera from patients with intestinal (n=123) and all but one from those with urinary schistosomiasis (n=35) had antibodies against Sm31/32. These and additional data suggest that both specificity and sensitivity of Sm31/32 to detect schistosome infections are close to 100%. Antibodies against these proteins developed also in monkeys after experimental infection with S. haematobium. Sm31/32 antigens reacted in immunoblots as a doublet with most S. haematobium-patient sera and as a broad band with many S. mansoni-sera suggesting that at least two components are present in the molecular weight region of Mr31/32 000. The data demonstrate the potential use of Sm31/32 from adult worms to diagnose patients with intestinal or urinary schistosomiasis in endemic areas.

Keywords

diagnostic proteinsimmunoblotspatient seraSchistosoma haematobiumSchistosoma mansoniserodiagnosisSm31/32
Type
Research Papers
Information
Journal of Helminthology , Volume 62 , Issue 2 , June 1988 , pp. 95 - 101
Copyright
Copyright © Cambridge University Press 1988

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References

REFERENCES

Batteiger, B., Newhall, W. J. & Jones, R. B. (1982) The use of Tween 20 as a blocking agent in the immunological detection of proteins transferred to nitrocellulose membranes. Journal of Immunological Methods, 55, 297–307.CrossRefGoogle ScholarPubMed
Butterworth, A. E., Capron, M., Cordingley, J. S., Dalton, P. R., Dunne, D. W., Kariuki, H. C., Kimani, G., Koech, D., Mugambi, M., Ouma, J. H., Prentice, M. A., Richardson, B. A., Arap, Siongok T. K., Sturrock, R. F. & Taylor, D. W. (1985) Immunity after treatment of human schistosomiasis mansoni. II. Identification of resistant individuals and analysis of their immune responses. Transactions of the Royal Society of Tropical Medicine and Hygiene, 79, 393–408.CrossRefGoogle ScholarPubMed
Dunne, D. W., Lucas, S., Bickle, Q., Pearson, S., Madgwick, L., Bain, J. & Doenhoff, M. J. (1981) Identification and partial purification of an antigen (Omega 1) from Schistosoma mansoni eggs which is putatively hepatotoxic in T-cell deprived mice. Transactions of the Royal Society of Tropical Medicine and Hygiene, 75, 54–71.Google Scholar
Hawkes, R., Niday, E. & Gordon, J. (1982) Dot immunobinding assay for monoclonal and other antibodies. Analytical Biochemistry, 119, 142–147.CrossRefGoogle ScholarPubMed
Klinkert, M. Q., Ruppel, A. & Beck, E. (1987) Cloning of diagnostic 31/32 kilodalton antigens of Schistosoma mansoni. Molecular and Biochemical Parasitology, 25, 247–255.Google Scholar
Korte, R., Schmidt-Ehry, B., Kielmann, A. A. & Brinkmann, U. K. (1986) Cost and effectiveness of different approaches to schistosomiasis control in Africa. Tropical Medicine and Parasitology, 37, 149–152.Google ScholarPubMed
Mitchell, G. F. & Cruise, K. M. (1986) Schistosomiasis: antigens and host-parasite interactions. In: Parasite antigens (editor, Pearson, T. W.) pp. 257–316. Marcel Dekker, Inc.: New York and Basel.Google Scholar
Mott, K. E. (1984) Diagnostic tools for Schistosoma japonicum control programmes. Arzneimittel-Forschung/Drug Research, 34, 1217–1220.Google ScholarPubMed
Mott, K. E. & Dixon, H. (1982) Collaborative study on antigens for immunodiagnosis of schistosomiasis. Bulletin of the World Health Organization, 60, 729–753.Google Scholar
Mott, K. E., Dixon, H., Carter, C. E., Garcia, E., Ishii, A., Matsuda, H., Mitchell, G., Owhashi, M., Tanaka, H. & Tsang, V. C. (1987) Collaborative study on antigens for immunodiagnosis of Schistosoma japonicum infection. Bulletin of the World Health Organization, 65, 233–244.Google Scholar
Mott, K. E., Dixon, H., Osei-Tutu, E., England, E. C., Ekue, K. & Tekle, A. (1985) Indirect screening for Schistosoma haematobium infection: a comparative study in Ghana and Zambia. Bulletin of the World Health Organization, 63, 135–142.Google Scholar
Ruppel, A., Breternitz, U. & Burger, R. (1987a) Diagnostic Mr 31,000 Schistosoma mansoni proteins: requirement of infection, but not immunization, and use of the “mini-blot” technique for the production of monoclonal antibodies. Journal of Helminthology, 61, 95–101.CrossRefGoogle Scholar
Ruppel, A., Burger, R., Shi, Y. E., Wei, D. X., Dell, R. & Diesfeld, H. J. (1986a) Schistosoma japonicum: Parasite proteins with a potential for diagnosis of human infections (abstract). Immunobiology, 173, 252.Google Scholar
Ruppel, A., Diesfeld, H. J. & Rother, U. (1985) Immunoblot analysis of Schistosoma mansoni antigens with sera of schistosomiasis patients: diagnostic potential of an adult schistosome polypeptide. Clinical and Experimental Immunology, 62, 499–506.Google Scholar
Ruppel, A., Shi, Y. E., Wei, D. X. & Diesfeld, H. J. (1987b) Sera of Schistosoma japonicum-infected patients cross-react with diagnostic 31/32 kD proteins of S. mansoni. Clinical and Experimental Immunology, 69, 291–298.Google Scholar
Ruppel, A., Shi, Y. E., Wei, D. X., Rother, U. & Diesfeld, H. J. (1986b) Antibodies of patients infected with Schistosoma japonicum crossreact with diagnostic proteins of Schistosoma mansoni. Journal of the Tongji Medical University, 6, 138–143.Google Scholar
Simpson, A. J. G. & Smithers, S. R. (1985) Schistosomes: surface, egg and circulating antigens. Current Topics in Microbiology and Immunology, 120, 205–239.Google ScholarPubMed
Smithers, S. R. & Terry, R. J. (1965) The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of adult worms. Parasitology, 55, 695–700.CrossRefGoogle ScholarPubMed
Teesdale, C. H. & Amin, M. A. (1976) Comparison of the Bell technique, a modified Kato thick smear technique, and a digestion method for the field diagnosis of schistosomiasis mansoni. Journal of Helminthology, 50, 17–20.CrossRefGoogle Scholar