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Diagnosis of the strongyloid nematode Strongyloides venezuelensis in experimentally infected rats

Published online by Cambridge University Press:  14 July 2015

P.D. Marques ,
F.M. Malta ,
D.M.C.L. Meisel ,
M.A. Corral ,
J.R. Pinho ,
J.M. Costa-Cruz ,
P.P. Chieffi ,
R.C.B. Gryschek  and
F.M. Paula
P.D. Marques
Affiliation:
Instituto de Medicina Tropical de São Paulo
F.M. Malta
Affiliation:
Laboratório de Gastroenterologia e Hepatologia Tropical, Hospital das Clínicas da Faculdade de Medicina
D.M.C.L. Meisel
Affiliation:
Laboratório de Investigação Médica (LIM-06), Hospital das Clínicas da Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil
M.A. Corral
Affiliation:
Laboratório de Investigação Médica (LIM-06), Hospital das Clínicas da Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil
J.R. Pinho
Affiliation:
Laboratório de Gastroenterologia e Hepatologia Tropical, Hospital das Clínicas da Faculdade de Medicina
J.M. Costa-Cruz
Affiliation:
Laboratório de Diagnóstico de Parasitoses, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil
P.P. Chieffi
Affiliation:
Faculdade de Ciências Médicas, Santa Casa, São Paulo, Brazil
R.C.B. Gryschek
Affiliation:
Laboratório de Investigação Médica (LIM-06), Hospital das Clínicas da Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil
F.M. Paula*
Affiliation:
Laboratório de Investigação Médica (LIM-06), Hospital das Clínicas da Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil
*
* E-mail: [email protected]
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Abstract

Strongyloides venezuelensis is an intestinal nematode of rats, frequently used as a model for studying human and animal strongyloidiasis. In the present study, we evaluated parasitological, serological and molecular methods for the diagnosis of experimental S. venezuelensis in rats, Rattus norvegicus. Blood and faecal samples were collected and analysed up to 60 days post infection (pi) with adult worm recovery occurring from 5 to 45 days pi. Using an enzyme-linked immunosorbent assay (ELISA), serum levels of IgG antibodies increased up to 28 days pi, thereafter decreasing by day 60 pi. Polymerase chain reaction (PCR) assays detected S. venezuelensis DNA in faecal samples of rats from 5 to 21 days pi. The present study therefore represents the first step towards improving the diagnosis of experimental strongyloidiasis.

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 4 , July 2016 , pp. 422 - 427
Copyright
Copyright © Cambridge University Press 2015 

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