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Diagnosis of Schistosoma bovis infection in cattle by an indirect haemagglutination test

Published online by Cambridge University Press:  05 June 2009

J. M. Preston
Affiliation:
Veterinary Research Laboratories, P.O. Kabette, Kenya
W. P. H. Duffus
Affiliation:
Veterinary Research Laboratories, P.O. Kabette, Kenya

Abstract

An indirect haemagglutination test has been developed for the detection of Schistosoma bovis antibody. Fresh, formalin and glutaraldehyde fixed sheep erythrocytes as well as fresh and formalin fixed human erythrocytes were tested. Tanned glutaraldehyde fixed sheep erythrocytes sensitized with an adult worm extract were the most sensitive and remained stable for at least 8 weeks at 4°C. With such cells 98% of a group of S. bovis infected cattle were positive while negative results were obtained from cattle infected with other helminths.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1975

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References

REFERENCES

Bing, D.H., Weyand, J.G.M. and Stavitsky, A.B. (1967) Haemagglutination with aldehyde-fixed erythrocytes for assay of antigens and antibodies. Proceedings of the Society of experimental Biology and Medicine, 124, 11661170.CrossRefGoogle ScholarPubMed
Butler, W.T. (1963) Haemagglutination studies with formalinized erythrocytes. Effect of diazo-benzidine and tannic acid treatment on sensitization by soluble antigen. Journal of Immunology, 90, 663671.CrossRefGoogle ScholarPubMed
Duffus, W.P.H., Preston, J.M. and Staak, C.H. (1974) Initial fractionation of adult Schistosoma bovis antigen for diagnosis of infection in cattle. Journal of Helminthology, 49, 17.Google Scholar
Duffus, W.P.H. and Wagner, G.G. (1975) Immuno-chemical studies on East Coast Fever. III. Development of an indirect haemagglutination assay using Theileria parva piroplasm antigen. Journal of Parasitology, (In press).Google Scholar
Garabedian, G.A., Matossian, R.M. and Djanian, A.Y. (1957) An indirect haemagglutination test for hydatid disease. Journal of Immunology, 78, 269272.Google Scholar
Goodger, B.V. (1971) Preparation and preliminary assessment of purified antigen in the passive haemagglutination test for bovine babesiosis. Australian Veterinary Journal, 47, 251256.CrossRefGoogle ScholarPubMed
Hoshino, S., Camargo, M.E. and Da Silva, L.C. (1970) Standardization of a haemagglutinatiou test for schistosomiasis with formalin-treated human erythrocytes. The American Journal of tropical Medicine and Hygiene, 19, 463470.CrossRefGoogle ScholarPubMed
Kagan, I.G. (1955) Haemagglutination after immunisation with schistosome antigens. Science, 122, 376377.CrossRefGoogle ScholarPubMed
Kagan, I.G. (1968) Serologic diagnosis of schistosomiasis. Bulletin of the New York Academy of Medicine, 44, 262277.Google ScholarPubMed
Kagan, I.G. and Bargai, U. (1956) Studies on the serology of trichinosis with haemagglutination, agar diffusion tests, and precipitin ring tests. Journal of Parasitology, 42, 237245.CrossRefGoogle Scholar
Kagan, I.G. and Oliver-Gonzáles, J. (1955) Preliminary studies on haemagglutination in schistosomiasis. Journal of Parasitology, 41, (Supplement) 26.Google Scholar
Kagan, I.G. and Oliver-González, J. (1958) Haemagglutination studies with schistosome antigens. Journal of Parasitology, 44, 457460.CrossRefGoogle ScholarPubMed
Kagan, I.G. and Pellegrino, J. (1961) A critical review of Immunological methods for the diagnosis of bilharziasis. Bulletin of the World Health Organisation, 25, 611674.Google ScholarPubMed
Kagan, I.G., Rairigh, D.W. and Kaiser, R.L. (1962) A clinical, parasitologic and immunological study in 103 Peurto Rican males residing in the United States. Annals of International Medicine, 56, 457460.CrossRefGoogle Scholar
Weichselbeum, T.E. (1946) An accurate and rapid method for the determination of proteins in small amounts of serum and plasma. American Journal of Clinical Pathology, 16, 140146.Google Scholar