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Development of Microphalloides japonicus (Osborn, 1919) metacercariae in vitro (Trematoda: Microphallidae)

Published online by Cambridge University Press:  05 June 2009

Takahiro Fujino ,
Fusanori Hamajima ,
Yoichi Ishii  and
Ryoichi Mori
Takahiro Fujino
Affiliation:
Department of Parasitology, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan
Fusanori Hamajima
Affiliation:
Department of Parasitology, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan
Yoichi Ishii
Affiliation:
Department of Parasitology, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan
Ryoichi Mori
Affiliation:
Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan
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Abstract

Metacercariae of the trematode Microphalloides japonicus (Osborn, 1919) were cultivated in various media to gravid adults, and the results were compared with those in vivo. A few eggs were produced in the uterus even in balanced salt solution, although cultivation in Eagle's MEM and NCTC 109 media was much better. Addition of heat-inactivated serum resulted in even better development. Encysted metacercariae also produced eggs in some media, but most of them were incomplete and without shells.

Type
Research Article
Information
Journal of Helminthology , Volume 51 , Issue 2 , June 1977 , pp. 125 - 129
Copyright
Copyright © Cambridge University Press 1977

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References

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