Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-19T21:55:25.306Z Has data issue: false hasContentIssue false
  • English
  • Français

Detection of serum cytokines before and after pharmacological and surgical treatment in patients with cystic echinococcosis

Published online by Cambridge University Press:  02 March 2015

M.I. Naik ,
R.K. Tenguria  and
E. Haq
M.I. Naik*
Affiliation:
Department of Microbiology, Govt. Motilal Vigyan MahavidyalyaBhopal462 008MP, India
R.K. Tenguria
Affiliation:
Department of Microbiology, Govt. Motilal Vigyan MahavidyalyaBhopal462 008MP, India
E. Haq
Affiliation:
Department of Biotechnology, University of Kashmir, Hazratbal Srinagar-Jammu and Kashmir-190 006, India
*
* E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Human cystic echinococcosis (CE), caused by Echinococcus granulosus, is one of the most important and widespread parasitic zoonoses. One of the problems that can be encountered after treating CE patients is the risk of post-surgical relapses or treatment failure, thus a long-term clinical and serological follow-up is required to evaluate the success or failure of therapy. In the present study immunological markers have been identified to indicate the effectiveness of pharmacological and surgical treatments. The relationship between serum cytokine levels and the outcome of chemotherapy and surgery was evaluated in 50 patients with CE. Serum interleukin (IL)-4, IL-10 and interferon-gamma (IFN-γ) concentrations were determined by enzyme-linked immunosorbent assay (ELISA) before and after pharmacological and surgical treatment. Serum cytokine levels of IL-4, IL-10 and IFN-γ were elevated in a significant proportion of patients during the active stage of disease. IL-4, IL-10 and IFN-γ were measurable in 41 (82%), 37 (74%) and 25 (50%) patients before the treatment. Clinical and radiological assessment of patients 2 years after pharmacological treatment has shown that 48 of 50 patients responded to treatment. IL-4 and IL-10 levels were decreased significantly (P< 0.05) in these patients. Conversely, patients who did not respond showed high levels of IL-4 and IL-10 and undetectable levels of IFN-γ. Hence these results suggest that serum IL-4 and IL-10 detection may be useful in the follow-up of patients with CE.

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 1 , January 2016 , pp. 91 - 95
Copyright
Copyright © Cambridge University Press 2015 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Brunetti, E., Kern, P. & Vuitton, D.A. (2010) Expert consensus for the diagnosis and treatment of cystic and alveolar echinococcosis in humans. Acta Tropica 114, 1–16.CrossRefGoogle ScholarPubMed
Fauser, S. & Kern, P. (1997) T-lymphocyte cytokine mRNA expression in cystic echinococcosis. Acta Tropica 64, 35–51.CrossRefGoogle ScholarPubMed
Haralabidis, S., Karagouni, E., Frydas, S. & Dotsika, E. (1995) Immunoglobulin and cytokine profile in murine secondary hydatidosis. Parasite Immunology 17, 625–630.CrossRefGoogle ScholarPubMed
Hernandez-Pomi, A., Borras-Salvador, R. & Mir-Gisbert, A. (1997) Analysis of cytokine and specific antibody profiles in hydatid patients with primary infection and relapse of disease. Parasite Immunology 19, 553–561.CrossRefGoogle ScholarPubMed
Horton, R.J. (1989) Chemotherapy of Echinococcus infection in man with albendazole. Transactions of the Royal Society of Tropical Medicine and Hygiene 83, 97–102.CrossRefGoogle ScholarPubMed
Junghanss, T., da Silva, A.M., Horton, J., Chiodini, P.L. & Brunetti, E. (2008) Clinical management of cystic echinococcosis: state of the art, problems and perspectives. American Journal of Tropical Medicine and Hygiene 79, 301–311.CrossRefGoogle ScholarPubMed
McManus, D.P. & Bryant, C. (1995) Biochemistry, physiology and molecular biology of Echinococcus.. pp. 355–410in Thompson, R.C. & Lymbery, A.J. (Eds) The biology of Echinococcus and hydatid disease. Wallingford, UK, CAB International.Google Scholar
Mezioug, D. & Touil-Boukoffa, C. (2009) Cytokine profile in human hydatidosis: possible role in the immunosurveillance of patients infected with Echinococcus granulosus. Parasite 16, 57–64.CrossRefGoogle ScholarPubMed
Mosmann, T.R. & Moore, K.W. (1991) The role of IL-10 in cross regulation of Th1 and Th2 responses. Immunology Today 12, 49–53.CrossRefGoogle Scholar
Ortona, E., Rıgano, R., Buttarı, B., Delunardo, F., Ioppolo, S., Marguttı, P., Profumo, E., Teggı, A., Vaccarı, S. & Sıracusano, A. (2003) An update on immunodiagnosis of cystic echinococcosis. Acta Tropica 85, 165–171.CrossRefGoogle ScholarPubMed
Pang, N., Zhang, F., Ma, X., Zhang, Z., Zhao, H., Xin, Y., Wang, S., Zhu, Y., Wen, H. & Ding, J. (2014) Th9/IL-9 profile in human echinococcosis: their involvement in immune response during infection by Echinococcus granulosus. Mediators of Inflammation doi:10.1155/2014/781649.CrossRefGoogle ScholarPubMed
Rigano, R., Buttari, B., De Falco, E., Profumo, E., Ortona, E., Margutti, P., Scotta, C., Teggi, A. & Siracusano, A. (2004) Echinococcus granulosus-specific T-cell lines derived from patients at various clinical stages of cystic echinococcosis. Parasite Immunology 26, 45–52.CrossRefGoogle ScholarPubMed
Rigano, R., Profumo, E., Di Felice, G., Ortona, E., Teggi, A. & Siracusano, A. (1995a) In vitro production of cytokines by peripheral blood mononuclear cells from hydatid patients. Clinical Experimental Immunology 99, 433–439.CrossRefGoogle ScholarPubMed
Rigano, R., Profumo, E., Ioppolo, S., Notargiacomo, S., Ortona, E., Teggi, A. & Siracusano, A. (1995b) Immunological markers indicating the effectiveness of pharmacological treatment in human hydatid disease. Clinical Experimental Immunology 102, 281–285.CrossRefGoogle ScholarPubMed
Rigano, R., Profumo, E., Buttari, B., Teggi, A. & Siracusano, A. (1999a) Cytokine gene expression in peripheral blood mononuclear cells (PBMC) from patients with pharmacologically treated cystic echinococcosis. Clinical Experimental Immunology 118, 95–101.CrossRefGoogle ScholarPubMed
Rigano, R., Profumo, E., Ioppolo, S., Notargiacomo, S., Teggi, A. & Siracusano, A. (1999b) Serum cytokine detection in the clinical follow up of patients with cystic echinococcosis. Clinical Experimental Immunology 115, 503–507.CrossRefGoogle ScholarPubMed
Rigano, R., Profumo, E., Eruschi, F., Carulli, G., Azzara, A., Ioppolo, S., Buttari, B., Ortona, E., Margutti, P., Teggi, A & Siracusano, A. (2001) Modulation of human immune response by Echinococcus granulosus antigen B and its possible role in evading host defenses. Infection and Immunity 69, 288–296.CrossRefGoogle ScholarPubMed
Rogan, M.T. & Craig, P.S. (1997) Immunology of Echinococcus granulosus infections. Acta Tropica 67, 7–17.CrossRefGoogle ScholarPubMed
Shan, J.Y., Ji, W.Z., Li, H.T., Tuxun, T., Lin, R.Y. & Wen, H. (2011) TLR2 and TLR4 expression in peripheral blood mononuclear cells of patients with chronic cystic echinococcosis and its relationship with IL-10. Parasite Immunology 33, 692–696.CrossRefGoogle ScholarPubMed
Sher, A., Fiorentino, D., Caspar, P., Pearce, E. & Mosmann, T. (1991) Production of IL-10 by CD4+T lymphocytes correlates with down-regulation of Th1 cytokine synthesis in helminth infection. Journal of Immunology 15, 2713–2716.CrossRefGoogle Scholar
Teggi, A., Lastilla, M.G. & De Rosa, F. (1993) Therapy of human hydatid disease with Mebendazole and Albendazole. Antimicrobial Agents Chemotherapy 37, 1679–1684.CrossRefGoogle ScholarPubMed
Todorov, T., Vutova, K., Mechkov, G., Petkov, D., Nedelkov, G. & Tonchev, Z. (1990) Evaluation of response to chemotherapy of human cystic echinococcosis. British Journal of Radiology 63, 523–531.CrossRefGoogle ScholarPubMed
Torcal, J., Navarro-Zorraquino, M., Lozano, R., Larrad, L., Salinas, J.C., Ferrer, J., Roman, J. & Pastor, C. (1996) Immune response and in vivo production of cytokines in patients with liver hydatidosis. Clinical and Experimental Immunology 106, 317–322.CrossRefGoogle ScholarPubMed
Touil-Boukoffa, C., Sanceau, J., Tayebi, B. & Wietzerbin, J. (1997) Relationship among circulating interferon, tumor necrosis factor-alpha and interleukin-6, and serologic reaction against parasitic antigen in human hydatidosis. Journal of Interferon and Cytokine Research 17, 211–217.CrossRefGoogle ScholarPubMed
Tuxun, T., Wang, J.H., Lin, R.Y., Shan, J.Y., Tai, Q.W., Li, T., Zhang, J.H., Zhao, J.M. & Wen, H. (2012) Th17/Treg imbalance in patients with liver cystic echinococcosis. Parasite Immunology 34, 520–527.CrossRefGoogle ScholarPubMed
Vuitton, D.A. (2003) The ambiguous role of immunity in echinococcosis: protection of the host or of the parasite? Acta Tropica 85, 119–132.CrossRefGoogle ScholarPubMed
Wen, H., New, R.R.C. & Craig, P.S. (1993) Diagnosis and treatment of human hydatidosis. British Journal of Clinical Pharmacology 35, 565–574.CrossRefGoogle ScholarPubMed
WHO-IWGE (World Health Organization Informal Working Group on Echinococcosis). (1996) Guidelines for treatment of cystic and alveolar echinococcosis in humans. Bulletin of the World Health Organization 74, 231–242.Google Scholar
Zhang, W. & McManus, D.P. (2006) Recent advances in the immunology and diagnosis of echinococcosis. FEMS Immunology and Medical Microbiology 47, 24–41.CrossRefGoogle ScholarPubMed
Zhang, W., Ross, A.G. & McManus, D.P. (2008) Mechanisms of immunity in hydatid disease: implications for vaccine development. Journal of Immunology 181, 6679–6685.CrossRefGoogle ScholarPubMed