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Detection of inducible nitric oxide synthase in Schistosoma japonicum and S. mansoni

Published online by Cambridge University Press:  12 April 2024

X.-C. Long ,
M. Bahgat ,
K. Chlichlia ,
A. Ruppel  and
Y.-L. Li
X.-C. Long
Affiliation:
Department of Tropical Hygiene and Public Health, University of Heidelberg, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany, : Department of Parasitology, Tongji Medical College, Huazhong University of Science and Technology, Hangkong Lu 13, Wuhan, 430030, P. R. China
M. Bahgat*
Affiliation:
Department of Tropical Hygiene and Public Health, University of Heidelberg, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany, :
K. Chlichlia
Affiliation:
Department of Tropical Hygiene and Public Health, University of Heidelberg, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany, :
A. Ruppel
Affiliation:
Department of Tropical Hygiene and Public Health, University of Heidelberg, Im Neuenheimer Feld 324, 69120, Heidelberg, Germany, :
Y.-L. Li*
Affiliation:
Department of Parasitology, Tongji Medical College, Huazhong University of Science and Technology, Hangkong Lu 13, Wuhan, 430030, P. R. China
*
†Present address: Department of Medicinal Chemistry, National Research Centre, Dokki, Cairo, Egypt
*Author for correspondence Fax: 86 27 83692608 Email: [email protected]
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Abstract

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Schistosoma japonicum and S. mansoni were tested for reactivity with an anti-inducible nitric oxide (iNOS) antibody and the distribution of iNOS was studied by immunofluorescent tests in different stages of the parasites. Reactivity was associated with the tegument in both larval schistosomes (sporocysts and cercariae) and eggs. With adult worms, the majority of the immunofluorescence was predominantly subtegumental in S. japonicum and parenchymal in S. mansoni. Fluorescence was also observed in host tissues (snails and mouse liver). In Western blots, the enzyme of S. japonicum had an apparent molecular weight of about 210 kDa. The possible role of worm and host iNOS in the parasite–host interrelation remains to be clarified.

Type
Review Article
Information
Journal of Helminthology , Volume 78 , Issue 1 , March 2004 , pp. 47 - 50
Copyright
Copyright © Cambridge University Press 2004

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