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Detection of circulating immune complexes in the sera of dogs infected with Dirofilaria immitis, by Clq-binding enzyme-linked immunosorbent assay

Published online by Cambridge University Press:  05 June 2009

K. Matsumura ,
Y. Kazuta ,
R. Endo ,
K. Tanaka  and
T. Inoue
K. Matsumura
Affiliation:
Yamaguchi Prefectural Research Institute of Health, 2-5-67 Aoi Yamaguchi 753, Japan
Y. Kazuta
Affiliation:
Yamaguchi Prefectural Research Institute of Health, 2-5-67 Aoi Yamaguchi 753, Japan
R. Endo
Affiliation:
Yamaguchi Prefectural Research Institute of Health, 2-5-67 Aoi Yamaguchi 753, Japan
K. Tanaka
Affiliation:
Yamaguchi Prefectural Research Institute of Health, 2-5-67 Aoi Yamaguchi 753, Japan
T. Inoue
Affiliation:
Department of Veterinary Medicine, Faculty of Agriculture, Yamaguchi University, Yoshida Yamaguchi 753 Japan
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Abstract

The presence of circulating immune complexes (CIC) in the sera of dogs infected with Dirofilaria immitis was detected by using a Clq-binding enzyme-linked immunosorbent assay. Specificity of this assay with different concentrations of heat-aggravated canine IgG (ACG) was observed, i.e., the ELISA readings, expressed as ug equivalents ACG/ml, increased with increasing amounts of ACG. The intra-assay variability was below 10%. The CIC levels of infected and uninfected dogs were 177–0± 104–7 ug/ml and 22–8±45–8 ng/ml (mean±SD), respectively. The highest level was observed in 12 dogs with amicrofilaraemic infection. Age distribution of CIC levels in the 23 infected dogs also showed a significant positive correlation. These findings suggested that the CIC are present in the sera of dogs with dirofilariasis and may relate to canine glomerulonephritis.

Keywords

Dirofilaria immitisdogELISAcirculating immune complexes
Type
Research Article
Information
Journal of Helminthology , Volume 60 , Issue 3 , September 1986 , pp. 239 - 243
Copyright
Copyright © Cambridge University Press 1986

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