Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-11-25T07:47:49.688Z Has data issue: false hasContentIssue false

Cytochemistry of Mehlis' gland in Ceylonocotyle scoliocoelium

Published online by Cambridge University Press:  18 November 2009

P. N. Sharma
Affiliation:
Department of Zoology, University of Udaipur, Udaipur-313 001, India
S. Mandawat
Affiliation:
Department of Zoology, University of Udaipur, Udaipur-313 001, India
A. N. Sharma
Affiliation:
Department of Zoology, University of Udaipur, Udaipur-313 001, India

Abstract

Histochemical techniques were used to study the chemical composition of Mehlis’ gland secretion in Ceylonocotyle scoliocoelium. The results suggest the secretion to be lipoproteinous in nature, containing several hydrolytic and oxidative enzymes. In tests for nine hydrolytic enzymes only seven were detected in relation to gland cells, ihe two enzymes not showing any reactivity being glucose-6-phosphatase and 5-Nucleotidase. Alkaline phosphatase and ATPase have been associated with the permeability and transport of precursors of synthetic material from the surrounding parenchyma and with eliminating the waste products from the gland: ACPase in (i) generating a favourable milieu in the gland for the synthesis of secretory material and (ii) in regulating secretion and its over production; TPPase in elaborating the secretion, esterases in the metabolism of the gland. Intense SDH and MAO activities were related to high synthetic activity of the gland cells. Phenol oxidase—a key enzyme involved in tanning was absent.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1981

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bancroft, J. D. (1967) An introduction to histochemical technique. Appleton Century-Crofts; New York.Google Scholar
Barka, T. & Anderson, P. J. (1963) Histochemistry: Theory, practice and bibliography. Harper and Row Publishers: New York, Evanston and London.Google Scholar
Bogitsh, B. J. (1970) Observations on the cytochemistry of the Mehlis’ gland cells of Haematoloechus medioplexus Journal of Parasitology, 56, 10841094.CrossRefGoogle Scholar
Burton, P. R. (1963) A histochemical study of vitelline cells, egg capsules and Mehlis’ gland in the frog lung fluke, Haematolochus medioplexus. Journal of Experimental Zoology, 154, 247258.CrossRefGoogle ScholarPubMed
Burton, P. R. (1967) Fine structure of the reproductive system of a frog lung fluke I. Mehlis’ gland and associated ducts. Journal of Parasitology, 53, 540555.CrossRefGoogle ScholarPubMed
Clegg, J. A. (1965) Secretion of lipoprotein by Mehlis* gland in Faschla hepatica. Annals of the New York Academy of Sciences, 118, 969986.CrossRefGoogle ScholarPubMed
De Duve, E. (1970) CIBA Foundation Symposium on Lysosomes, J. A. Churchill: London.Google Scholar
Ebrahimzadeh, A.(1966)HistologischeUntersuchungenuberdenFcinbaudesOogenotopbeidigenen Trematoden. Zeitschrift fur Parasitenkunde, 27, 127168.Google Scholar
Friend, D. S. & Murray, M. J. (1965) Osmium impregnation of the Golgi apparatus. American Journal of Anatomy, 117, 135141.CrossRefGoogle ScholarPubMed
Gleener, G. G., Burtner, H. J. and Brown, G. W. (1957) The histochemical demonstration of monoamine oxidase activity by tetrazolium salts. Journal of Histochemistry and Cytochemistry, 5, 591596.CrossRefGoogle Scholar
Halton, D. W. (1967) Histochemical studies of carboxylic esterase activity in Fasciola hepatica. Journal of Parasitology, 53, 12101216.CrossRefGoogle ScholarPubMed
Hanumantha-Rao, K. (1959) Histochemistry of Mehlis’ gland and egg-shell formation in the liver fluke Fasciola hepatica L. Experientia, 15, 464465.CrossRefGoogle ScholarPubMed
Karnovsky, M. & Roots, L. (1964) A direct coloring method for cholinesterases. Journal of Histochemistry and Cytochemistry, 12, 219221.CrossRefGoogle ScholarPubMed
Mandawat, S. & Sharma, P. N. (1978a) Histochemical distribution of Acetyl and Butyryl cholinesterases in the tissues of a trematode “Paramphistomum cervi”. Indian Journal of Experimental Biology, 16, 968972.Google ScholarPubMed
Mandawat, S. & Sharma, P. N. (1978b) Histochemical distribution and significance of non-specific esterase in the tissues of Paramphistomum cervi (Trematoda; Digenea). Indian Journal of Parasitology, 2, 107110.Google Scholar
Madhvi, R. (1968) Diplodiscus Mehrai: Chemical nature of egg-shell. Experimental Parasitology, 23, 392397.CrossRefGoogle Scholar
Mcmanus, J. F. A. & Mowry, R. W. (1960) Staining methods, histologic and histochemical. New York, Hoeber Medical Division, Harper and Row.Google Scholar
Nachlas, M. M., Tsou, K. C.Desouza, E.Cheng, C. S. & Seliyman, A. M. (1957) Cytochemical demonstration of succinic dehydrogenase by the use of a new p-dinitrophenyl substituted diteltrazole. Journal of Histochemistry and Cytochemistry. 5, 420436.CrossRefGoogle Scholar
Novikoff, A. B. & Goldfischer, (1961) Nucleosidediphosphatase activity in the Golgi apparatus and its usefulness for cytological studies. Proceedings of the National Academy of Sciences of the United States of America, 47, 802810.CrossRefGoogle ScholarPubMed
Pearse, A. G. E. (1968) Histochemistry, theoretical and applied, Vol. 1, 2nd edit., London: J. A. Churchill Ltd.Google Scholar
Pearse, A. G. E. (1972) Histochemistry, theoretical and applied, Vol. 2, 3rd edit., London: J. A. Churchill.Google Scholar
Pearse, A. G. E. & Scarpelli, D. G. (1958) Cytochemical localization of succinic dehydrogenase in mitochondria of Pcriplanata americana. Nature, 181, 125144.CrossRefGoogle ScholarPubMed
Padykula, H. A. & Herman, E. (1955) The specificity of the histochcmical method for adenosine triphosphatase. Journal of Histochemistry and Cytochemistry, 3, 170.CrossRefGoogle ScholarPubMed
Reader, T. A. J. (1976) Studies on the infrastructure, histochemistry and cytochemistry of the uninfected digestive gland of Bithynia tentaculata (Mollusca: Gastropoda) and on the ultrastructure of this host organ in snails infected with larval digeneans. Zeitschrift fur Parasitenkunde, 50, 1130.CrossRefGoogle Scholar
Sharma, P. N. (1976) Histochemical studies on the distribution of alkaline phosphatase, acid phosphatase, 5-nucleotidase and ATPase in various reproductive tissues of certain digenetic trematodes. Zeitschrift fur Parasitenkunde. 49, 223231.CrossRefGoogle ScholarPubMed
Sharma, P. N. (1978a) Histochemical localization of succinate dehydrogenase in the lymphatic system of a trematode Ceylonocolyle scolicoelium. Journal of Helminthology, 52, 159162.CrossRefGoogle Scholar
Sharma, P. N. (1978b) Chemoarchitectonics of prostate gland of two digenetic trematodes. Current Science, 47, 877879.Google Scholar
Sharma, P. N. (1979) Histochemical localization of glycogen, lipids, proteins and phosphatases in the parenchyma and other tissues of some digenetic trematodes. Indian Journal of Experimental Biology, 17, 479483.Google Scholar
Sharma, P. N. & Sood, P. P. (1978) Histochemical distribution and functional significance of some enzymes on the suckers of certain digenetic trematodes. Indian Journal of Parasitology, 2, 7982.Google Scholar
Smyth, J. D. (1966) “The physiology of Trematodes. University reviews in biology”. Oliver and Boyd: Edinburgh and London.Google Scholar
Smyth, J. D. & Clegg, J. A. (1959) Egg shell formation in trematodes and cestodes. Experimental Parasitology, 8, 286323.CrossRefGoogle ScholarPubMed
Spence, I. M. & Silk, M. H.(1971)Ultrastructural studies of the blood fluke Schistosoma mansoni W.The Mehlis’ gland. South African Journal of Medical Science, 36, 6976.Google Scholar
Thorsell, W. & Bjorkman, N. (1965) On the fine structure of the Mehlis’ gland cells in the liver fluke Fasciola hepatica L. Zeitschrift fur Parasitenkunde, 26, 6370.Google ScholarPubMed
Threadgold, L. T. & Irwin, S. W. B. (1970) Electron microscope studies of Fasciola hepatica IX. The fine structure of Mehlis’ gland. Zeitschrift fur Parasitenkunde, 35, 1630.CrossRefGoogle ScholarPubMed
Ujiie, N. (1936) On the process of egg-shell formation of Clonorchis sinetisis a liver fluke. Journal of the Medical Association of Formosa, 35, 18941896.Google Scholar
Wachstein, M. & Meisel, E. (1957) Histochemistry of hepatic phosphatases at a physiological pH with special reference to the demonstration of bile canaliculi. American Journal of Clinical Pathalogy. 27,13.CrossRefGoogle Scholar