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Cyclic adenosine 3′, 5′-monophosphate in Gastrothylax crumenifer and Explanatum explanatum

Published online by Cambridge University Press:  05 June 2009

H. Ehsan ,
K.A. Rashid ,
W. Van Dongen  and
H.A. Van Onckelen
H. Ehsan
Affiliation:
Department of Biology, University of Antwerp, B-2610 Antwerp, Belgium
K.A. Rashid
Affiliation:
Department of Biochemistry, University of Antwerp, B-2610 Antwerp, Belgium:
W. Van Dongen
Affiliation:
Department of Chemistry, Nucleoside Research and Mass Spectrometry Unit, University of Antwerp, Groenenbogerlaan 171, B-2020 Antwerp, Belgium
H.A. Van Onckelen
Affiliation:
Department of Biology, University of Antwerp, B-2610 Antwerp, Belgium
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Abstract

A rapid and reliable method based on immuno-affinity chromatography and preparative high pressure liquid chromatography was used for extraction and purification of cAMP from the two trematode species Gastrothylax crumenifer and Explanatum explanatum. The purified cAMP was quantified by electrospray tandem mass spectrometry in an amount of 44 and 83 picomole/g fresh weight of Gastrothylax crumenifer and Explanatum explanatum respectively.

Type
Research Papers
Information
Journal of Helminthology , Volume 71 , Issue 2 , June 1997 , pp. 147 - 149
Copyright
Copyright © Cambridge University Press 1997

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