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Crustacean-borne infections with microphallid metacercariae (Digenea: Microphallidae) from focal areas in Meghalaya, north-east India

Published online by Cambridge University Press:  22 May 2012

L.M. Goswami ,
P.K. Prasad ,
D.K. Biswal ,
A. Chatterjee  and
V. Tandon
L.M. Goswami
Affiliation:
Department of Zoology, North-Eastern Hill University, Shillong 793022, Meghalaya, India Department of Zoology, Nowgong College, Nagaon 782001, Assam, India
P.K. Prasad
Affiliation:
Department of Zoology, North-Eastern Hill University, Shillong 793022, Meghalaya, India Department of Science and Technology, New Mehrauli Road, New Delhi 110016, India
D.K. Biswal
Affiliation:
Bioinformatics Centre, North-Eastern Hill University, Shillong 793022, Meghalaya, India
A. Chatterjee
Affiliation:
Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong 793022, Meghalaya, India
V. Tandon*
Affiliation:
Department of Zoology, North-Eastern Hill University, Shillong 793022, Meghalaya, India
*
*Fax: +91 364 2550 300 E-mail: [email protected]
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Abstract

During a survey of edible Crustacea for recovery of infective stages (metacercariae) of potential helminthozoonoses of trematode origin in north-east India, the crab species Barytelphusa lugubris mansoniana, collected from suspected foci of lungfluke infection in Meghalaya and Assam, was found to harbour metacercarial cysts that were different from the earlier reported infection, in which the lungfluke Paragonimus was confirmed to be implicated. Using morphological criteria, this metacercaria was identified as Microphallus indicus Mukherjee & Ghosh, 1967 of the trematode family Microphallidae. The present study extends the previous work by providing molecular characterization of this parasite using ribosomal internal transcribed spacer regions (rDNA ITS1 and ITS2) and the partial large ribosomal subunit DNA, lsr. These target regions were amplified by polymerase chain reaction (PCR) using trematode universal primers and sequenced. In BLAST analysis the query sequences were found close to members of Microphallidae and closest to the genus Microphallus.

Type
Research Papers
Information
Journal of Helminthology , Volume 87 , Issue 2 , June 2013 , pp. 222 - 229
Copyright
Copyright © Cambridge University Press 2012 

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