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Copro-PCR prevalence of Echinococcus granulosus infection in dogs in Kerman, south-eastern Iran

Published online by Cambridge University Press:  30 January 2017

S.R. Mirbadie
Affiliation:
School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
H. Kamyabi
Affiliation:
Research Center for Hydatid Disease in Iran, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
M.A. Mohammadi
Affiliation:
Department of Medical Laboratory Sciences, Sirjan School of Medical Sciences, Kerman University of Medical Sciences, Kerman, Iran
S. Shamsaddini
Affiliation:
Research Center for Hydatid Disease in Iran, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
M.F. Harandi*
Affiliation:
Research Center for Hydatid Disease in Iran, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
*
*Fax: +98-34-332 57478 E-mail: [email protected]

Abstract

The main objective of this study was to determine the prevalence of taeniid parasites and the specific detection of Echinococcus granulosus using copro-DNA polymerase chain reaction (PCR) analysis in the stray dogs of Kerman, south-eastern Iran. From September 2013 to May 2014, faecal samples of stray dogs were collected from different parts of the city of Kerman and its suburbs. Faecal samples from dogs were collected randomly within 24 h of defecation. All samples were transferred to the research lab and coprological examinations were conducted by the formalin–ether concentration method. In the microscopically positive samples, mitochondrial cytochrome c oxidase subunit 1 (cox1) specific primers were used to determine the taeniid identity of the infection. In addition, another set of primers was used for the specific diagnosis of E. granulosus sensu lato. In total, 307 faecal samples from stray dogs were examined for the presence of the parasites. Taeniidae eggs were detected in 34 dogs (11.07%). All 34 taeniid-positive specimens were PCR positive for cox1 (444 bp). Of all taeniid-positive specimens, 21 samples (6.8% of all dog specimens) were positive according to primers specific for E. granulosus. The findings of the present study revealed that canine echinococcosis is prevalent in the stray dogs in Kerman. The findings of the present study have important implications for hydatid control programmes in the area.

Type
Research Papers
Copyright
Copyright © Cambridge University Press 2017 

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References

Abbasi, I., Branzburg, A., Campos-Ponce, M., Hafez, S.K.A., Raoul, F., Craig, P.S. & Hamburger, J. (2003) Copro-diagnosis of Echinococcus granulosus infection in dogs by amplification of a newly identified repeated DNA sequence. American Journal of Tropical Medicine and Hygiene 69, 324330.Google Scholar
Al-Jawabreh, A., Dumaidi, K., Ereqat, S., Nasereddin, A., Al-Jawabreh, H., Azmi, K., Al-Laham, N. & Abdeen, Z. (2015) Incidence of Echinococcus granulosus in domestic dogs in Palestine as revealed by copro-PCR. PLOS Neglected Tropical Diseases 9, 3934.CrossRefGoogle ScholarPubMed
Al-Sabi, M.N.S., Kapel, C.M.O., Deplazes, P. & Mathis, A. (2007) Comparative copro-diagnosis of Echinococcus multilocularis in experimentally infected foxes. Parasitology Research 101, 731736.CrossRefGoogle ScholarPubMed
Beiromvand, M., Akhlaghi, L., Massom, S.H.F., Mobedi, I., Meamar, A.R., Oormazdi, H., Motevalian, A. & Razmjou, E. (2011) Detection of Echinococcus multilocularis in carnivores in Razavi Khorasan province, Iran using mitochondrial DNA. PLOS Neglected Tropical Diseases 5, 1379.Google Scholar
Borji, H., Naghibi, A. & Jahangiri, F. (2013) Copro-DNA test for diagnosis of canine echinococcosis. Comparative Clinical Pathology 22, 909912.Google Scholar
Bowles, J., Blair, D. & McManus, D.P. (1992) Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Molecular and Biochemical Parasitology 54, 165173.CrossRefGoogle ScholarPubMed
Bruzinskaite, R., Sarkunas, M., Torgerson, P.R., Mathis, A. & Deplazes, P. (2009) Echinococcosis in pigs and intestinal infection with Echinococcus spp. in dogs in southwestern Lithuania. Veterinary Parasitology 160, 237241.Google Scholar
Budke, C.M., Carabin, H., Ndimubanzi, P.C., Nguyen, H., Rainwater, E., Dickey, M., Bhattarai, R., Zeziulin, O. & Qian, M.B. (2013) A systematic review of the literature on cystic echinococcosis frequency worldwide and its associated clinical manifestations. American Journal of Tropical Medicine and Hygiene 88, 10111027.CrossRefGoogle ScholarPubMed
Harandi, M.F., Moazezi, S.S., Saba, M., Grimm, F., Kamyabi, H., Sheikhzadeh, F., Sharifi, I. & Deplazes, P. (2011) Sonographical and serological survey of human cystic echinococcosis and analysis of risk factors associated with seroconversion in rural communities of Kerman, Iran. Zoonoses and Public Health 58, 582588.CrossRefGoogle ScholarPubMed
Harandi, M.F., Budke, C.M. & Rostami, S. (2012) The monetary burden of cystic echinococcosis in Iran. PLOS Neglected Tropical Diseases 6, e1915.Google Scholar
Hartnack, S., Budke, C.M., Craig, P.S., Jiamin, Q., Boufana, B., Campos-Ponce, M. & Torgerson, P.R. (2013) Latent-class methods to evaluate diagnostic tests for Echinococcus infections in dogs. PLOS Neglected Tropical Diseases 7, e2068.Google Scholar
Lahmar, S., Lahmar, S., Boufana, B., Bradshaw, H. & Craig, P.S. (2007) Screening for Echinococcus granulosus in dogs: comparison between arecoline purgation, coproELISA and coproPCR with necropsy in pre-patent infections. Veterinary Parasitology 144, 287292.Google Scholar
Mirzaei, M. & Fooladi, M. (2012) Prevalence of intestinal helminthes in owned dogs in Kerman city, Iran. Asian Pacific Journal of Tropical Medicine 5, 735737.CrossRefGoogle Scholar
Mobedi, I., Zare Bidaki, M., Siavashi, M.R., Naddaf, S.R., Kia, E.B. & Mahmoudi, M. (2013) Differential detection of Echinococcus spp. copro-DNA by nested-PCR in domestic and wild definitive hosts in Moghan Plain, Iran. Iranian Journal of Parasitology 8, 107113.Google Scholar
Moss, J.E., Chen, X., Li, T., Qiu, J., Wang, Q., Giraudoux, P., Ito, A., Torgerson, P.R. & Craig, P. (2013) Reinfection studies of canine echinococcosis and role of dogs in transmission of Echinococcus multilocularis in Tibetan communities, Sichuan, China. Parasitology 140, 16851692.Google Scholar
Oba, P., Ejobi, F., Omadang, L., Chamai, M., Okwi, A.L., Othieno, E., Inangolet, F.O. & Ocaido, M. (2015) Prevalence and risk factors of Echinococcus granulosus infection in dogs in Moroto and Bukedea districts in Uganda. Tropical Animal Health and Production 48, 249254.Google Scholar
Rokni, M. (2008) The present status of human helminthic diseases in Iran. Annals of Tropical Medicine and Parasitology 102, 283295.Google Scholar
Rostami, S., Salavati, R., Beech, R.N., Sharbatkhori, M., Babaei, Z., Saedi, S. & Harandi, M.F. (2013a) Cytochrome c oxidase subunit 1 and 12S ribosomal RNA characterization of Coenurus cerebralis from sheep in Iran. Veterinary Parasitology 197, 141151.CrossRefGoogle ScholarPubMed
Rostami, S., Talebi, S., Babaei, Z., Sharbatkhori, M., Ziaali, N., Rostami, H. & Harandi, M.F. (2013b) High resolution melting technique for molecular epidemiological studies of cystic echinococcosis: differentiating G1, G3, and G6 genotypes of Echinococcus granulosus sensu lato. Parasitology Research 112, 34413447.Google Scholar
Rostami, S., Salavati, R., Beech, R.N., Babaei, Z., Sharbatkhori, M., Baneshi, M.R., Hajialilo, E., Shad, H. & Harandi, M.F. (2015a) Molecular and morphological characterization of the tapeworm Taenia hydatigena (Pallas, 1766) in sheep from Iran. Journal of Helminthology 89, 150157.Google Scholar
Rostami, S., Salavati, R., Beech, R.N., Babaei, Z., Sharbatkhori, M. & Harandi, M.F. (2015b) Genetic variability of Taenia saginata inferred from mitochondrial DNA sequences. Parasitology Research 114, 13651376.Google Scholar
Sharifi, I. & Zia-Ali, N. (1996) The present status and intensity of Echinococcus granulosus infection in 391 stray dogs in rural and urban areas of the city of Kerman, Iran. Iranian Journal of Public Health 25, 1320.Google Scholar
Statistical Center of Iran (2015) Available at http://amar.sci.org.ir (accessed 4 June 2015).Google Scholar
Stefanic, S., Shaikenov, B.S., Deplazes, P., Dinkel, A., Torgerson, P.R. & Mathis, A. (2004) Polymerase chain reaction for detection of patent infections of Echinococcus granulosus (‘sheep strain’) in naturally infected dogs. Parasitology Research 92, 347351.Google Scholar
Ziadinov, I., Mathis, A., Trachsel, D., Rysmukhambetova, A., Abdyjaparov, T.A., Kuttubaev, O.T., Deplazes, P. & Torgerson, P.R. (2008) Canine echinococcosis in Kyrgyzstan: using prevalence data adjusted for measurement error to develop transmission dynamics models. International Journal for Parasitology 38, 11791190.Google Scholar