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A combination of two Brugia malayi filarial vaccine candidate antigens (BmALT-2 and BmVAH) enhances immune responses and protection in jirds

Published online by Cambridge University Press:  04 January 2011

S.B. Anand ,
K.N. Kodumudi ,
M.V. Reddy  and
P. Kaliraj
S.B. Anand
Affiliation:
Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai – 625021, India
K.N. Kodumudi
Affiliation:
Centre for Biotechnology, Anna University, Chennai – 600025, India
M.V. Reddy
Affiliation:
Centre for Biotechnology, Anna University, Chennai – 600025, India
P. Kaliraj*
Affiliation:
Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, India
*
*E-mail: [email protected]
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Abstract

In this study filarial recombinant protein or DNA vaccine constructs encoding BmALT-2 and BmVAH as single or as cocktail antigens were evaluated. Male jirds were immunized intramuscularly with DNA vaccine constructs or were immunized intraperitoneally with protein vaccine. The single and bicistronic DNA constructs induced substantial interferon-γ responses in spleen cells; antigen-specific responses were higher following immunization with the bicistronic cocktail construct and evoked a significant protective response of 57% in jirds challenged with Brugia malayi that was similar in the antibody-dependent cellular cytotoxicity (ADCC) assay and micropore chamber experiment. The cocktail protein vaccines induced a mixture of IgG2a (Th1) and IgG1 (Th2) responses with 80% protective response when challenged with B. malayi infective larvae. However, the single protein vaccine rALT-2 induced Th2 (IgG1/IgG3) with a 70% protective response and rVAH induced Th1 (IgG2a) with a lower proliferative response with 60% protection following challenge with B. malayi infective larvae. These results suggest that filarial cocktail protein vaccines are able to elicit substantial immune and protective responses when compared with single antigen vaccination in suitably vaccinated jirds.

Type
Research Papers
Information
Journal of Helminthology , Volume 85 , Issue 4 , December 2011 , pp. 442 - 452
Copyright
Copyright © Cambridge University Press 2010

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