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Characterization of the Opisthorchis viverrini genome

Published online by Cambridge University Press:  05 June 2009

R. Sermswan ,
S. Mongkolsuk  and
S. Sirisinha
R. Sermswan
Affiliation:
Faculty of Science, Mahidol University, Rama 6 Rd, Bangkok 10400, Thailand
S. Mongkolsuk
Affiliation:
Departments of Microbiology and Biotechnology, Faculty of Science, Mahidol University, Rama 6 Rd, Bangkok 10400, Thailand
S. Sirisinha*
Affiliation:
Faculty of Science, Mahidol University, Rama 6 Rd, Bangkok 10400, Thailand
*
**To whom correspondence should be addressed.
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Abstract

The methylations of trematode genomic DNA were analyzed using restriction enzymes and Southern blot hybridization. Restriction enzymes MspI, HpaII, HhaI were used to probe CpG methylation while MboI, Sau3A, DpnI were used for A methylation. The results revealed that Opisthorchis viverrini, Fasciola gigantica and Gigantocotyle siamensis had neither CpG nor A methylations. The presence of highly repeated DNA elements was also demonstrated in O. viverrini genomic DNA.

Keywords

Opisthorchis viverrinigenomeDNArestriction enzymes
Type
Research Note
Information
Journal of Helminthology , Volume 65 , Issue 1 , March 1991 , pp. 51 - 54
Copyright
Copyright © Cambridge University Press 1991

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References

REFERENCES

Maniatis, T., Fritsch, E. F. & Sambrook, J. (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, New York, pp. 544.Google Scholar
McManus, D. P., Knight, M. & Simpson, A. J. G. (1985) Isolation and characterization of nucleic acids from the hydatid organisms, Echinococcus spp. (Cestoda). Molecular and Biochemical Parasitology, 16, 251–266.CrossRefGoogle ScholarPubMed
Pollac, Y., Katzen, A.L., Spira, D.T. & Golenser, J. (1982) The genome of Plasmodium falciparum I: DNA base composition. Nucleic Acids Research, 10, 539–546.CrossRefGoogle Scholar
Razin, A. & Riggs, A.D. (1980) DNA methylation and gene function. Science, 210, 604–610.CrossRefGoogle ScholarPubMed
Simpson, A. J. G., Sher, A. & McCutchan, T. E. (1982) The genome of Schistosoma mansoni: Isolation of DNA, its size, bases and repetitive sequences. Molecular and Biochemical Parasitology, 6, 125–137.CrossRefGoogle ScholarPubMed
Singer, M.F. (1982) Highly repeated sequences in mammalian genomes. lnternational Review of Cytology, 76, 67–112.CrossRefGoogle ScholarPubMed
Southern, E. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology, 98, 503–505.CrossRefGoogle ScholarPubMed
Vanyushin, B.F. (1984) Replicative DNA methylation in animals and higher plants. In: Current Topics in Microbiology and Immunology (editor, Trautner, T. A.), 108, pp. 99–114, Springer-Verlag, Berlin: Germany.Google Scholar
Wongratanacheewin, S., Rattanasiriwilai, W., Priwan, R. & Sirisinha, S. (1987) Immunodepression in hamster experimentally infected with Opisthorchis viverrini. Journal of Helminthology, 61, 151–156.CrossRefGoogle ScholarPubMed