Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-22T06:03:46.189Z Has data issue: false hasContentIssue false
  • English
  • Français

Brugia pahangi in nude mice: protective immunity to infective larvae is Thy 1·2+ cell dependent and cyclosporin A resistant

Published online by Cambridge University Press:  05 June 2009

Ann C. Vickery  and
Jai K. Nayar
Ann C. Vickery
Affiliation:
College of Public Health, University of South Florida, Tampa, Florida 33612, USA
Jai K. Nayar
Affiliation:
Florida Medical Entomology Laboratory, IFAS-University of Florida, Vero Beacn, Florida, USA
Get access Rights & Permissions [Opens in a new window]

Abstract

Mechanisms of protective immunity to larvae of Brugia pahangi were studied in congenitally athymic nude C3H/HeN mice and their syngeneic heterozygous littermates. An average 11% if subcutaneous larval inocula was recovered from control nudes 28 days after inoculation. No worms were recovered from nude recipients of viable splenic Thy 1·2+ T lymphocytes from heterozygotes which had killed a priming does of B. pahangi larvae. Primed T lymphocytes, depleted of either Lyt 1·1+of Lyt 2·1+cells or incubated with anti-Thy 1.2 monoclonal antibody and complement, failed to protect nude mic against a larval challenge. Nor were primed B lymphocytes depleted by Thy 1·2+T cell contaminants protective. Treatment with cyclosporin A (CsA) did not increase the numbers of worms recovered from heterozygotes nor did CsA treatment of heterozygous cell donor abolish the ability of primed Thy 1·2+T lymphocytes to transfer protection to nude mice. IgG but not IgM antibody titres to B. pahangi antigens were depressed in all CsA-treated mice. CsA treatment of nude mice had no direct effect upon development of B. pahagi larvae. These results show that ptotective immunity to larvae of B. pahangi in mice depends upon small numbers of Thy 1·2+T cells which are CsA-resistant.

Keywords

Brugia pahangiMousecyclosporinProtective immunity
Type
Research Papers
Information
Journal of Helminthology , Volume 61 , Issue 1 , March 1987 , pp. 19 - 27
Copyright
Copyright © Cambridge University Press 1987

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Andrus, L. & Lafferty, K. J. (1982) Inhibition of T-cell activity by Cyclosporin A. Scandinavian Journal of Immunology, 15, 449–458.CrossRefGoogle Scholar
Blair, L. S. & Campbell, W. C. (1981) Immunization of ferrets against Dirofilaria immitis by means of chemically abbreviated infections. Parasite Immunology, 3, 143–147.CrossRefGoogle ScholarPubMed
Bout, D. T., Deslee, D. & Capron, A. R. (1984) Protection against schistosomiasis produced by cyclosporin A. American Journal of Tropical Medicine and Hygiene, 33, 185–186.CrossRefGoogle ScholarPubMed
Bout, D., Haque, A. & Capron, A. (1985) Filaricidal effects of cyclosporin-A against Dipetalonema viteae in Mastomys natalensis. Transactions of the Royal Society of Tropical Medicine and Hygiene, 78, 670–671.CrossRefGoogle Scholar
Chusattayanond, D. & Denham, D. A. (1984) Induction of host resistance to Brugia pahangi in jirds (Meriones unguiculatus) protected by chemoprophylaxis, Journal of Helminthology, 58, 245–249.CrossRefGoogle ScholarPubMed
Gui, X-E, Ho, M., Camp, P.E. (1982) Effect of cyclosporin A on murine natural killer cells. Infection and Immunity, 36, 1123–1127.CrossRefGoogle ScholarPubMed
Hayashi, Y., Nogami, S., Nakamura, M., Sirasaka, A. & Noda, K. (1984) Passive transfer of protective immunity against Brugia malayi in BALB/C mice. Japanese Journal of Experimental Mdeicine, 54, 13–187.Google ScholarPubMed
Kunkl, A. & Klaus, G. G. B. (1980) Selective effects of cyclosporin A on functional B cell subsets in the mouse. Journal of Immunology, 125, 2526–2531.CrossRefGoogle ScholarPubMed
Maccabe, R. E., Remington, J. S. & Araujo, F. G. (1985) In vivo and in vitro effects of cyclosporin A on Trypanosoma cruzi. American Journal of Tropcial Medicine and Hygiene, 34, 861–865.CrossRefGoogle Scholar
Mcintosh, L. C. & Thomson, A. W. (1980) Activity of the mononuclear phagocyte system in cyclosporin A-treated mice. Transplantation, 30, 384–386.CrossRefGoogle ScholarPubMed
Mehta, K., Sindhu, R. K., Subrahmanyam, D., Hopper, K., Nelson, D. S. & Rao, C. K. (1981) Antibody-dependent cell-mediated effects of bancroftian filariasis. Immunology, 43, 117–123.Google ScholarPubMed
Nilsson, L. -A., Lindblad, R., Olling, S. & Ouchterlony, O. (1985) The effect of cyclosporin A on the course of murine infection by Schistosoma mansoni. Parasite Immunology, 7, 19–27.CrossRefGoogle ScholarPubMed
Nutman, T. B., Ottesen, E.A., Fauci, A.S. & Volkman, D.J. (1984) Parasite antigen-specific human T cell lines and clones. The Journal of Clinical Investigation. 73, 1754–1762.CrossRefGoogle Scholar
Oothuman, P., Denham, D.A., Mcgreevy, P.B., Nelson, G. S. & Rogers, R. (1979) Successful vaccination of cats against Brugia pahangi with larvae attenuated by irradiation with 10 Krad cobalt 60. Parasite Immunology, 1, 209–216.CrossRefGoogle ScholarPubMed
Ottesen, E. A. (1984) Immunological aspects of lymphatic filariasis and onchocerciasis in man. Transactions of the Royal Society of Tropical Medicine and Hygiene, 78 (Supplement), 9–18.CrossRefGoogle ScholarPubMed
Philipp, M., Worms, M.J., Maizels, R.M. & ogilvie, B.M. (1984) Rodent models of filariasis. In: Contemporary Topices in Immunobiology (editor, Marchalonis, J. J.) pp. 275–321. Plenum Publishing Corporation: New York.Google Scholar
Rao, G.Y.V.B., Mehta, K. & Subrahmanyam, D. (1977) Litomosoides carinii: effect pf irradiation on the development and immunogenicity of the larval form. Experimental Parasitology, 43, 39–44.CrossRefGoogle Scholar
Shevach, E.M. (1985) The effects of cyclosporin A on the immune system. Annual Reviews of Immunology, 3, 397–423.CrossRefGoogle ScholarPubMed
Sim, B.K.L., Kwa, B.H. & Mak, J.W. (1982) Immune responses in human Brugia malayi infections: serum dependent cell-mediated destruction of infective larvae in vitro. Transactions of the Royal Society of Tropical medicine and Hygiene, 78 (supplement), 9–18.Google Scholar
Storey, D.M. & Al-Mukthar, A.S. (1982) Vaccination of jirds, Meriones unguiculatus against Litomosoides carinii and Brugia pahangi using irradiated larvae of L. carinii. Tropenmedizinund Parasitologie, 33, 23–24.Google ScholarPubMed
Thomson, A.W., Moon, D.K. & Nelson, D.S. (1983) Suppression of delayed-type hypersensitivity reactions and lymphokine production by cyclosporin A in the mouse. Clinical Experimental Immunology, 52, 599–606.Google ScholarPubMed
Vickery, A.C., Nayar, J.K. & Tamplin, M.L. (1985) Diethylcarbamazine-mediated clearance of Brugia pahangi microfilariae in immunodeficient nude mice. American Journal of Tropical Medicine and Hygiene, 34, 476–483.CrossRefGoogle ScholarPubMed
Vickery, A.C. & Vincent, A.L. (1984) Immunity to Brugia pahangi in athymic nude and normal mice: eosinophilia, antibody and hypersensitivity responses. Parasite Immunology, 6, 545–559.CrossRefGoogle ScholarPubMed
Vickery, A. C., Vincent, A. L. & Sodeman, W.A. Jr (1983) Effect of immune reconstitution on resistance to Brugia pahangi in congenitally athymic nude mice. Journal of Parasitology, 69, 478–485.CrossRefGoogle ScholarPubMed
Weinbaum, D. L., Kaplan, S. S., Zdziarski, V., Rinaldo, C. R. & Schroeder, K. K. (1984) Human polymorphonuclear leukocyte interaction with cyclosporine A. Infection and Immunity, 43, 791–794.CrossRefGoogle ScholarPubMed
Wong, M. M., Guest, M. F. & Lavoipierre, M. J. (1974) Dirofilaria immitis: fate and immunogenicity of irradiated infective stage larvae in beagles. Experimental Parasitology, 35, 465–47.CrossRefGoogle ScholarPubMed