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Blast cell activity in mice inflected with Hymenolepis nana, H. diminuta and Trichinella spiralis: in vivo uptake of 125IUdR in lymphoid tissues and gut

Published online by Cambridge University Press:  05 June 2009

F. Gabriele ,
A. R. Ecca ,
D. Wakelin  and
C. Palmas
F. Gabriele
Affiliation:
Istituto di Parassitologia, Facoltà di Medicina e chirurgia, Via della pineta 77, 09100 Cagliari, Italy.
A. R. Ecca
Affiliation:
Istituto di Parassitologia, Facoltà di Medicina e chirurgia, Via della pineta 77, 09100 Cagliari, Italy.
D. Wakelin
Affiliation:
Department of Zoology, University of Nottingham, University Park, Nottingham, NG7 2RD, England.
C. Palmas
Affiliation:
Istituto di Parassitologia, Facoltà di Medicina e chirurgia, Via della pineta 77, 09100 Cagliari, Italy.
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Abstract

The kinetics of the lymphoblast response in mice during the course of a primary infection with Hymenolepis nana was measured by the in vivo uptake of 125IUdR. The response was most marked in tissues local to the site of infection, involving to nodes draining the small intestine but not other areas, e.g., inguinal lymph nodes. A close correlation between these responses and the course of inflection was observed. Uptake of 125IUdR was greatest in the mesenteric lymph node (MLN) but the peak reached in this organ was later than that in Peyer's patches (PP), small intestine (SI) and spleen (S). The increase in lymphoblast activity of the MLN was similar with Trichinella spiralis; no significant blast cell response to inflection with H. diminuta was found till day 9 after injection, the results being similar to those obtained when H. nana inflections were established using cysticercoids rather than eggs. It has been shown that the increase in lymphoblast activity was closely correlated with the presence of cells which are most effective in adoptive transfer immunity. A dose-dependent effect was detected in blast cell activity of MLN in different inflection levels with T. spiralis and H. nana.

Keywords

Hymenolepis nanaHymenolepis diminutaTrichinella spiralislymphoblast responsemouse
Type
Research Papers
Information
Journal of Helminthology , Volume 60 , Issue 4 , December 1986 , pp. 313 - 321
Copyright
Copyright © Cambridge University Press 1986

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References

REFERENCES

Andreassen, J. (1981) Immunity to adult cestodes. Parasitology, 82, 153–159.Google Scholar
Asano, K., Nakamura, F. & Okamoto, K. (1982) Passive protection of node mice with lymphocytes from mice inflected with Hymenolepis nana eggs. Japanese Journal of Parasitology. 31, 391–400.Google Scholar
Befus, A. D. & O'Neill, M. & Bienestock, J. (1978) Immediate IgA precursor cells in rabbit intestinal lamina propria. Immunology, 35, 901–908.Google ScholarPubMed
Befus, A. D. & Podesta, R. B. (1976) Intestine. In: Ecological aspects of Parasitology, pp. 303–319 (Editor Kennedy, C. R.) North-Holland Publishing Company: Amsterdam.Google Scholar
Bienenstock, J. (1964) The physiology of the local innune response and the gastrointestinal tract. progress in Immunology II. 4, 197–207.Google Scholar
Dineen, J. K., Ronai, P. M. & Wagland, B. M. (1968).The cellular transfer of immunity to Trichostrongylus colubriformis in an isogenic strain of guinea-pig. IV. The localization of immnue lymphocytes in small intestine in inflected and non-inflected guinea-pigs. Immunology, 15, 671–679.Google Scholar
Dobson, C. & Soulsby, E. J. L. (1974) Lymphoid cell kinetics in guinea-pigs inflected with Trichostrongylus colubriformis; tritiated thymidine uptake un gut and allied lymphoid tissue, humoral IgE and hemagglutinating antibody responeses, delayed hypersensitivity reactions, and in vitro lymphocyte transformations during primary infections. Experimental Parasitology, 35, 16–34.CrossRefGoogle Scholar
Ferretti, G., Gabriele, F. & Palmas, C. (1980) Methodology in experimental inflections of mice with Hymenolepis nana. Bollettino di Zoologia, 47, 165–184.CrossRefGoogle Scholar
Ferretti, G., Gabriele, F., Palmas, C. & Wakelin, D. (1984) Interactions between Trichinella spiralis and Hymenolepi nana in the intestine of the mouse. International Journal for Parasitology, 14, 29–33.CrossRefGoogle ScholarPubMed
Grenics, R. K. & Wakelin, D. (1982) Short lived, dividing cells mediate adoptive transfer of immunity to Trichinella spiralis in mice. I. Availability of cells in primary and secondary inflections in relation to cellular changes in the mesenteric lymph-node. Immunology, 46, 443–450.Google Scholar
Hopkins, C. A., Subramanian, C. & Stallard, H. (1972) The development of Hymenolepis diminuta in primary and secondary inflections in mice. parasitology, 64, 401–412CrossRefGoogle Scholar
Larsh, J. E. Jr & Weatherly, N. F. (1975) Cell-mediated immunity against certain parasitic worms. Advances in Parasitology, 13, 183–222.CrossRefGoogle ScholarPubMed
Mayrhofer, G. & Fisher, R. (1979) IgA-containing plasma cells in the lamina propria of the gut: failure of a thoracic duct fistula to deplete the numbers in rat small intestine. European Journal of Immunology, 9, 85–90.CrossRefGoogle ScholarPubMed
Mitchell, G. F. (1980) T cell dependent effects in parasitic inflection and disease. Progress in Immunology. Vol. 4 (Editors Fougereau, M. F. and Dausset, J.) pp. 794–808. Academic Press: London.Google Scholar
Ogilvie, B. M. & Jones, V. E. (1973) Immunity in the parasitic relationship between helminths and hosts. Progress in Allergy, 17, 93–144.Google ScholarPubMed
Ogilvie, B. M. & Love, R. J. (1974) Cooperation between antobodies and cells in immunity to a namatode parasite. Transplantation Review, 19, 147–168.Google Scholar
Palmas, C., Wakelin, D. & Gabriele, F. (1984) Transfer of immunity against Hymenolepis nana in mice with lymphoid cells or serum from inflected donors. Parasitology, 89, 287–293.CrossRefGoogle ScholarPubMed
Parrotti, D. M. V. & Ferguson, A. (1974) Selective migration of lymphocytes within the mouse small intestine. Immunology, 26, 571–588.Google Scholar
Rose, M. L., Parrott, D. M. V. & Bruce, R. G. (1976) Migration of lymphoblasts to the small intestine. I. Effect of Trichinella spiralis inflection on the migration of mesenteric lymphoblasts and mesenteric T lymphoblasts in syngeneic mice. Immunology, 31, 723–730.Google Scholar
Soulsby, E. J. L. (1972) Cell-mediated immunity responses in parasitic inflections. In: Immunity to Animal Parasites. pp. 57–95. (Editor Soulsby, E. J. L.) Academic Press: New York and London.Google Scholar
Wakelin, D., Grenics, R. K. & Donachie, A. (1982) Short lived, dividing cells mediate adoptive transfer of immunity to Trichonella spiralis in mice. II. In vivo characteristics of the cells. Immunology, 46, 451–457.Google Scholar
Wakelin, D. & Lloyd, M. (1976) Accelerated expulsion of adult Trichinella spiralis in mice given lymphoid cells and serum from inflected donors. Parasitology, 72, 307–315.CrossRefGoogle Scholar
Wakelin, D. & Wilson, M. M. (1977) Transfer of immunity to Trichiella spiralis in the mouse with mesenteric lymph-node cells: time of appearance of effective cells in donors and expression if immunity in recipients. Parasitology, 74, 215–224.CrossRefGoogle ScholarPubMed