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Biological variation between two Brazilian geographical isolates of Echinostoma paraensei

Published online by Cambridge University Press:  12 April 2024

A. Maldonado Júnior ,
B.K. Zeitone ,
L.A. Amado ,
I.F. Amado ,
J.R. Machado-Silva  and
R.M. Lanfredi
A. Maldonado Júnior*
Affiliation:
Laboratório de Biologia e Controle da Esquistossomose, Departamento de Medicina Tropical, Instituto Oswaldo Cruz, Av. Brasil 4365 Manguinhos, 21 045-900, Rio de Janeiro, Brazil
B.K. Zeitone
Affiliation:
Laboratório de Biologia e Controle da Esquistossomose, Departamento de Medicina Tropical, Instituto Oswaldo Cruz, Av. Brasil 4365 Manguinhos, 21 045-900, Rio de Janeiro, Brazil
L.A. Amado
Affiliation:
Laboratório de Biologia e Controle da Esquistossomose, Departamento de Medicina Tropical, Instituto Oswaldo Cruz, Av. Brasil 4365 Manguinhos, 21 045-900, Rio de Janeiro, Brazil
I.F. Amado
Affiliation:
Laboratório de Helmintologia, Instituto de Biofísica Carlos Filho, CCS, bl G. UFRJ, Ilha do Fundão, 21 949-900, Rio de Janeiro, Brazil
J.R. Machado-Silva
Affiliation:
Laboratório de Helmintologia Romero Lascasas Porto, Departamento de Patologia e Laboratórios, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Rua Prof Manuel de Abreu, 444, 50 andar, Rio de Janeiro, Brazil
R.M. Lanfredi
Affiliation:
Laboratório de Helmintologia, Instituto de Biofísica Carlos Filho, CCS, bl G. UFRJ, Ilha do Fundão, 21 949-900, Rio de Janeiro, Brazil
*
*Fax: (011) 55 21 22803740 E-mail: [email protected]
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Abstract

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The biological behaviour and morphometric data from two allopatric isolates of Echinostoma paraensei (Rio Bonito – RB and Sumidouro – SU) collected from naturally infected Nectomys squamipes from two secluded Atlantic Forest fragments were studied. Mice that had been experimentally infected with ten encysted metacercariae of each isolate were monitored weekly in two trials to analyse worm burden and the kinetics of worm distribution along the intestine. The total number of uterine eggs, wet weights and measurements of the worms and body, acetabulum, testes and ovaries were also analysed. The RB isolate showed a higher worm burden, 7.7±0.8, and a longer life span, 16 weeks, compared to a worm burden of 5.8±1.1 and life span of 9 weeks for the SU isolate. Worms of the RB isolate were clustered in the duodenum and in the bile duct while the SU isolate worms were dispersed along the small intestine of infected mice. Both isolates developed similarly as regards morphometric data and wet weight, although the total number of uterine eggs was greater in RB. The degree of intraspecific variation observed in the worm distribution along the intestine, worm burden and life span raises questions regarding the use of these criteria for species differentiation. These findings suggest that variation in biological parameters found between the E. paraensei isolates could result from geographical isolation and, in particular, the environmental conditions of transmission. Further studies on E. paraensei polulations from different forest fragments will contribute towards an understanding of the speciation of this parasite.

Type
Review Article
Information
Journal of Helminthology , Volume 79 , Issue 4 , December 2005 , pp. 345 - 351
Copyright
Copyright © Cambridge University Press 2005

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