Hostname: page-component-cd9895bd7-gvvz8 Total loading time: 0 Render date: 2024-12-26T13:10:43.406Z Has data issue: false hasContentIssue false

Biochemical and histochemical studies of alkaline and acid phosphatases in a digenetic trematode, Pegosomum egretti

Published online by Cambridge University Press:  05 June 2009

Indra Rajvanshi
Affiliation:
Department of Zoology, Sukhadia University, Udaipur 313001, India.
K. L. Mali
Affiliation:
Department of Biochemistry, RNT Medical College, Udaipur 313001, India.

Abstract

The biochemistry and histochemistry of Pegosomum egretti have been studied using standard techniques. Phosphatases were analysed colorimetrically; the optimum pH for acid phosphatase activity was 5·0 and for alkaline phosphatase was 10·0. The results were compared with those of other trematodes. Histochemical localization of acid and alkaline phosphatases revealed differences in enzyme activity in various tissues. These differences in the site and pattern of distribution of the two enzymes have been discussed in relation to transport of raw materials and the metabolism of the cell concerned.

Type
Research Papers
Copyright
Copyright © Cambridge University Press 1986

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Barka, T. & Anderson, P. J. (1963) Histochemistry: Theory, practice and bibliography. New York: Hoeber, Medical Division, Harper and Row, Publishers.Google Scholar
Goil, M. M. (1966) Physiological studies on trematodes: phosphatase systems in Gastrothylax crumenifer. Parasitology, 56, 105110.CrossRefGoogle ScholarPubMed
Goil, M. M. (1973) Phosphatase systems in Fasciolopsis buski Lankester, 1857 and Gastrodiscus aegyptiacus Cobbold, 1876. Parasitology, 67, 197204.CrossRefGoogle ScholarPubMed
Goil, M. M. (1975) Phasphatase system in Paramphistomum explanatum Fischoeder. 1901. Zeitschrift für Parasitenkunde, 46, 211219.CrossRefGoogle Scholar
Gupta, A. N. (1970) Histochemical localization of alkaline phosphatase and its functional significance in the gut of digenetic trematodes. Acta Biologica Academiae Scientiarum Hungaricae. 21, 9197.Google ScholarPubMed
Gupta, S. P. & Gupta, R. C. (1977) Phosphatase activity in Ganeo tigrinum from Rana tigrina. Zeitschrift für Parasitenkunde. 54, 8994.CrossRefGoogle Scholar
Gupta, S. P. & Gupta, R. C. (1978) Phosphatase activity in Gastrodiscus aegyptiacus (Trematoda). Indian Journal of Parasitology, 2, 6163.Google Scholar
Gupta, V. & Agarwal, S. K. (1979) Phosphatase activity in Gastrothylax crumenifer (Trematoda). Indian Journal of Parasitology, 3, 5355.Google Scholar
Halton, D. W. (1966) Occurrence of microvilli-like structures in the gut of digenetic trematodes Experientia, 22, 828829.CrossRefGoogle ScholarPubMed
Halton, D. W. (1967) Studies on phosphatase activity in Trematoda. Journal of Parasitology, 53, 4654.CrossRefGoogle ScholarPubMed
Haque, M. & Siddiqi, A. H. (1982) Histochemical and electrophoretic studies on phosphatases of some Indian trematodes. Journal of Helminthology, 56, 111116.CrossRefGoogle ScholarPubMed
Hora, C. & Sharma, P. N. (1980) Comparative biochemical observation on glycogen, protein, lipids and enzymes of Ceylonocotyle scoliocoelium and Paramphistomum cervi, the rumen amphistomes of buffalo. Proceedings of the Indian Academy of Parasitology, 1, 125129.Google Scholar
King, E. J. & Wootton, I. D. P. (1959) Microanalysis in medical biochemistry. 3rd Edition. J. & A. Churchill Ltd.: London.Google Scholar
Lumsden, R. D. (1975) Surface ultrastructure and cytochemistry of parasitic helminths. Experimental Parasitology, 37, 267339.CrossRefGoogle ScholarPubMed
Ma, L. (1964). Acid phosphatase in Clonorchis sinensis. Journal of Parasitology, 50, 235240.CrossRefGoogle ScholarPubMed
Nizami, W. A., Siddiqi, A. H. & Yusufi, A. N. K. (1975) Non-specific alkaline phosphomonoesterases of eight species of digenetic trematodes. Journal of Helminthology, 49, 281287.CrossRefGoogle ScholarPubMed
Nollen, P. M. (1968) Autoradiographic studies on reproduction in Philophthalmus megalurus (Cort, 1914) (Trematoda). Journal of Parasitology, 54, 4348.CrossRefGoogle ScholarPubMed
Ohman, C. (1966) The structure and function of adhesive organ in strigeid trematodes IV. Holostephanus luhei Szidat, 1936. Parasitology, 56, 481491.CrossRefGoogle Scholar
Pappas, P. W. & Read, C. P. (1975) Membrane transport in helminth parasites. A review. Experimental Parasitology. 37, 469530.CrossRefGoogle ScholarPubMed
Parshad, V. R. & Guraya, S. S. (1978) Phosphatases in helminths: Effects of pH and various chemicals and anthelmintics on the enzyme activites. Veterinary Parasitology, 4, 111120.CrossRefGoogle Scholar
Pearse, A. G. E. (1968) Histochemistry – Theoretical and Applied. vol.1, J. A. Churchill Ltd.: London.Google Scholar
Probeet, A. J., Goil, M. M. & Sharma, R. K. (1972) Biochemical and histochemical studies on the non-specific phosphomonoesterases of Fasciola gigantica Cobbold 1855. Parasitology, 64, 347353.CrossRefGoogle Scholar
Sharma, P. N. (1976) Histochemical studies on the distribution of alkaline phosphatase, acid phosphatase, 5-nucleotidase and ATPase in various reproductive tissues of certion digenetic trematodes. Zeitschrift für Parasitenkunde, 49, 223231.CrossRefGoogle ScholarPubMed
Sharma, P. N. & Mandawat, S. (1982) A comparison of morphology, acid phosphatase and ATPase activity in Ganeo tigrinum from hibernating and non-hibernating Rana cyanophlyctis and Rana tigrina. Journal of Helminthology, 56, 510.CrossRefGoogle Scholar
Sharma, P. N., Mandawat, S. & Sharma, A. N. (1981) Cytochemistry of Mehlis' gland in Ceylonocotyle scoliocoelium. Journal of Helminthology, 55, 141148.CrossRefGoogle Scholar
Srivastava, O. N. (1957) A new species of Pegosomum, an echinostome (Trematoda) from cattle egret. Proceedings of the National Academy of Science, India, Section B. 27, 129134.Google Scholar
Srivastava, M. & Gupta, S. P. (1975) Phosphatase activity in Isoparorchis hypselobagri (Trematoda). Zoologischer Anzeiger 195, 3542.Google Scholar
Threadgold, L. T. (1968) Electron microscope studies of Fasciola hepatica, VI. The ultrastructural localization of phosphatases. Experimental Parasitology, 23, 264276.CrossRefGoogle ScholarPubMed
Von Brand, T. (1979) Biochemistry and physiology of endoparasites. Elsevier\North Holland Biomedical Press: Amsterdam, New York, Oxford.Google Scholar