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The antibody response to Dracunculus medinensis in an endemic human population of northern Ghana

Published online by Cambridge University Press:  05 June 2009

P. Bloch ,
P. E. Simonsen  and
B. J. Vennervald
P. Bloch
Affiliation:
Danish Bilharziasis Laboratory, Jaegersborg Alle I D, 2920 Charlottenlund, Denmark
P. E. Simonsen*
Affiliation:
Danish Bilharziasis Laboratory, Jaegersborg Alle I D, 2920 Charlottenlund, Denmark
B. J. Vennervald
Affiliation:
Danish Bilharziasis Laboratory, Jaegersborg Alle I D, 2920 Charlottenlund, Denmark
*
*Author for correspondence.
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Abstract

The serum antibody response (total, and isotypes IgG1, IgG4, IgM, IgA and IgE) to Guinea worm infection was examined in humans from a highly endemic area of northern Ghana by ELISA and SDSPAGE/Western blot techniques using an adult D. medinensis antigen. Sera were obtained early and late in the peak transmission period, from persons with patent and postpatent infections, as well as from persons from the same endemic area who claimed never to have had Guinea worm infection. To observe for potential cross-reactions in the tests, sera were also obtained from areas with no transmission of Guinea worm from patients with hookworm. O. volvulus and W. bancrofti infections, and from non-infected controls. Sera from persons living in the Guinea worm endemic area reacted extensively with Guinea worm antigen in both tests, and large numbers of bands were produced in the Western blots (up to 35 identified for some sera). For most antibody isotypes, the ELISA absorbance values obtained with sera from the same individuals varied between the two transmission seasons, with the highest titres present towards the end of the peak transmission period. The mean antibody titres for persons in the patent and postpatent infection categories were not significantly different when sera were obtained at the same season of the year. Persons from the endemic area, who claimed never to experience patent infections, also had antibodies to Guinea worm, although at significantly lower mean levels than for the patent and postpatent categories. The highest specificity in the ELISA and the most homogenous Western blots were obtained when detecting for antibodies of the IgG4 isotype.

Keywords

Drancunculus medinesisGuinea wormantibody isotypesELISAWestern blotimmunologyNematoda
Type
Research Papers
Information
Journal of Helminthology , Volume 67 , Issue 1 , March 1993 , pp. 37 - 48
Copyright
Copyright © Cambridge University Press 1993

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References

REFERENCES

Aiyedun, B. A., Browning, M. J. & Edungbola, L. D. (1985) Guinea worm in Kware State. II. Immunological studies on naturally infected subjects. East African Medical Journal, 62, 459–465.Google Scholar
Belcher, D. W., Wurapa, F. K., Ward, W. B. & Lourie, I. M. (1975) Guinea worm in Southern Ghana: Its epidemiology and impact on agricultural productivity. American Journal of Tropical Medicine and Hygiene, 24, 243–249.CrossRefGoogle ScholarPubMed
Bras, J. L., Duchatell, C., Payet, M. & Savel, J. (1977) Comparaison des résultats obtenus a partir d'antigénes homologues et hétérologues dans l'étude immunologique de l'onchocercose et de la dracuculose. Bulletin de la Société de Pathologic Exotique, 70, 515–524.Google Scholar
Bras, J. L., Duchatelle, C., Payet, M. & Savel, J. (1977) Comparaison des résultats obtenus a partir d'antigènes homologues et hétérologues dans l'étude immunologique de l'onchocercose et de la dracunculose. Bulletin de la Société de Pathologie Exotique, 70, 515–524.Google Scholar
Bras, J. L., Duchatelle, C., Andrieu, B. & Coulaud, J. P. (1980) Dracunculose, health. Journal of Tropical Pediatrics, 35, 285–288.Google Scholar
Fagbemi, B. O. & Hillyer, G. V. (1990) Immunodiagnosis of dracunculiasis by Falcon assay screening test-enzyme-linked immunosorbent assay (FAST-ELISA) and by enzyme-linked immunoelectrotransfer blot (EITB) technique. American Journal of Tropical Medicine and Hygiene, 43, 655–668.CrossRefGoogle ScholarPubMed
Fairley, N. H. & Liston, W. G. (1924) Studies in the pathology of dracontiasis. Part I. Indian Journal of Medical Research, 11, 915–932.Google Scholar
Garate, T., Kliks, M. M., Cabrera, Z. & Parkhouse, R. M. E. (1990) Specific and cross-reacting antibodies in human responses to Onchocerca volvulus and Dracunculus medinensis infections. American Journal of Tropical Medicine and Hygiene, 42, 140–147.CrossRefGoogle ScholarPubMed
Gentilini, M., Pinon, J. M., Raffier, G. & Niel, G. (1972) Résultats d'une étude sérologique de 356 sujets atteints de dracunculose explorés par la technique d'immunofluorescence indirecte. Bulletin de la Société de Pathologie Exotique, 65, 103–111.Google Scholar
Gilles, H. M. & Ball, P. A. J. (1964) Guinea-worm infection and gastric function. Annals of Tropical Medicine and Parasitology, 58, 78–82.CrossRefGoogle ScholarPubMed
Goka, A. K. J. & Farthing, M. J. G. (1987) The use of 3,3′, 5,5′ tetramethyl-benzidine as a peroxidase substrate in microplate enzyme linked immunosorbent assay. Journal of Immunoassay, 8, 29–41.CrossRefGoogle ScholarPubMed
Heegaard, N. H. H. & Bjerrum, O. J. (1988) Immunoblotting—General principles and procedures. In: CRC Handbook of Immunoblotting of Proteins (editors. Bjerrum, O. J. & Heegaard, N. H. H.) pp. 1–25. CRC Press Inc.: Florida.Google Scholar
Jain, R. C. & Gupta, O. P. (1966) Electrophoretic pattern of serum proteins in dracunculosis. Indian Journal of Medical Sciences, 20, 713–715.Google ScholarPubMed
Johnstone, A. & Thorpe, R. (1987) Immunochemistry in Practice. Blackwell Scientific Publications.Google Scholar
Kliks, M. M. & Rao, C. K. (1984) Development of rapid ELISA for early serodiagnosis of dracunculiasis. Journal of Communicable Diseases, 16, 287–294.Google ScholarPubMed
Kwan-Lim, G. E., Forsyth, K. P. & Maizels, R. M. (1990) Filarial-specific IgG4 response correlates with active Wuchereria bancrofti infection. Journal of Immunology, 145, 4298–4305.CrossRefGoogle ScholarPubMed
Kyhse-Andersen, J. (1984) Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose. Journal of Biochemical and Biophysical Methods, 10, 203–209.CrossRefGoogle ScholarPubMed
Lal, R. B. & Ottesen, E. A. (1988) Enhanced diagnostic specificity in human filariasis by IgG4 antibody assessment. Journal of infectious Diseases, 158, 1034–1037.CrossRefGoogle ScholarPubMed
Muller, R. (1970a) Dracunculus and dracunculiasis. Advances in Parasitology, 27, 73–151.Google Scholar
Muller, R. (1970b) Dracunculus medinensis: Diagnosis by indirect fluorescent antibody technique. Experimental Parasitology, 27, 357–361.CrossRefGoogle ScholarPubMed
Muller, R. (1991) Dracunculus in Africa. In: Parasitic Helminths and Zoonoses in Africa (editors, Macpherson, C. N. L. & Craig, P. S.) pp. 204–223. Unwin Hyman: London.Google Scholar
Perrudet-Badoux, A., Boiteau, A. & Gentilini, M. (1984) Filaire de médine sérodiagnostic différentiel par ELISA. Pathologic Biologie, 32, 182–184.Google ScholarPubMed
Ramsay, G. W. S. C. (1935) Observations on an intradermal test for dracontiasis. Transactions of the Royal Society of Tropical Medicine and Hygiene, 28, 399–404.CrossRefGoogle Scholar
Scott, D. (1959) An epidemiological note on Guinea-worm infection in North-West Ashanti, Ghana. Annals of Tropical Medicine and Parasitology, 54, 32–43.CrossRefGoogle Scholar
Sita, Devi C., Devasankaraiah, Narasiah I. L., Reddy, C. R. R. M. & Rao, V. (1969) Role of gastric acidity in Guinea-worm infection. Indian Journal of Medical Research, 57, 1850–1855.Google Scholar
Smith, G. S., Blum, D., Huttly, S. R. A., Okeke, N., Kirkwood, B. R. & Feachem, R. G. (1989) Disability from dracunculiasis: effect on mobility. Annals of Tropical Medicine and Parasitology, 83, 151–158.CrossRefGoogle ScholarPubMed
Stefanopoulo, G. J. & Daniaud, J. (1940) Réaction de fixation du complement et intradermoréaction au cours de las filariose humaine a Dr. medinensis. Bulletin de la Société de Pathologie Exotique, 33, 149–153.Google Scholar
Voller, A. & Savigny, D. (1981) Enzyme linked immunosorbent assay (ELISA). In: Techniques in Clinical Immunology (editor, Thompson, R. A.) pp. 157–169. Blackwell Scientific Publications.Google Scholar
Waits, S. J. (1987) Dracunculiasis in Africa in 1986: Its geographic extent, incidence, and at-risk population. American Journal of Tropical Medicine and Hygiene, 37, 119–125.CrossRefGoogle Scholar
Watts, S. J., Brieger, W. R., Yacoob, M. (1989) Guinea worm: an in-depth study of what happens to mothers, families and communities. Social Science and Medicine, 29, 1043–1049.CrossRefGoogle ScholarPubMed
Well, G. J., Ogunrinade, A. F., Chandrashekar, R. & Kale, O.O. (1990) IgG4subclass antibody serology for onchocerciasis. Journal of Infectious Diseases, 161, 549–554.CrossRefGoogle Scholar
World Health Organization (1989) Dracunculiasis. Ghana. Weekly Epidemiological Records, No. 3–20 January.Google Scholar