Hostname: page-component-586b7cd67f-2plfb Total loading time: 0 Render date: 2024-11-25T08:06:25.288Z Has data issue: false hasContentIssue false
  • English
  • Français

The antibody response in serum, intestinal wall and intestinal lumen of NMRI mice infected with Echinostoma caproni

Published online by Cambridge University Press:  05 June 2009

M. K. Agger ,
P. E. Simonsen  and
B. J. Vennervald
M. K. Agger
Affiliation:
Danish Bilharziasis Laboratory, Jaegershorg Alle I D, 2920 Charlottenlund, Denmark
P. E. Simonsen*
Affiliation:
Danish Bilharziasis Laboratory, Jaegershorg Alle I D, 2920 Charlottenlund, Denmark
B. J. Vennervald
Affiliation:
Danish Bilharziasis Laboratory, Jaegershorg Alle I D, 2920 Charlottenlund, Denmark
*
* Author for correspondence.
Get access Rights & Permissions [Opens in a new window]

Abstract

Groups of mice (NMRI) were infected with 0.6 and 25 metacercariae of the intestinal trematode Echinostoma caproni. An enzyme linked immunosorbent assay (ELISA) was developed for measuring the specific IgM, IgG and IgA antibody responses in serum, small intestinal tissue and small intestinal lumen of the mice, by using a crude adult E. caproni antigen. In infected mice, significant levels of IgM were measured in the sera from day 14 after infection and of IgG and IgA from day 28 after infection. Early in the infection, the mean level of serum IgM was higher in 6-worm infections than in 25-worm infections. Late in the infection, higher mean levels of IgA were reached in the serum of mice with high than in those with low dose infections. The onset of appearance of antibodies in the tissue of the small intestine reflected the picture seen in the serum. For IgM, and to a lesser degree for IgG, the highest mean antibody levels appeared in the posterior sections of the small intestinal wall, where also the parasites were located. The mean level of IgA, however, was uniform throughout the length of the small intestinal tissue. High levels of specific IgA were detected in the lumen of the small intestine on day 28 after infection, especially in the anterior sections, where only few parasites were located. No specific IgM or IgG could be detected in the intestinal lumen on this day. The results are related to the intestinal location and the pattern of expulsion of E. caproni in the mouse host.

Keywords

Ehinostoma capronimiceserum antibody responseintestinal antibody responseELISATrematoda
Type
Research Papers
Information
Journal of Helminthology , Volume 67 , Issue 3 , September 1993 , pp. 169 - 178
Copyright
Copyright © Cambridge University Press 1993

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Alghali, S. O. (1987) Hymenolepis citelli, H. diminuta and H. microstoma: immunoglobulin-containing cells in the lamina propria of the mouse gut during primary and secondary infections. Journal of Helminthology, 61, 329–340.CrossRefGoogle Scholar
Andresen, K., Simonsen, P. E., Andersen, B. J. & Birch-Andersen, A. (1989) Echinostoma caproni in mice: Shedding of antigens from the surface of an intestinal trematode. International Journal for Parasitology, 19, 111–118.CrossRefGoogle ScholarPubMed
Befus, A. D. (1977) Hymenolepis diminuta and H. microstoma: Mouse immunoglobulins binding to the tegumental surface. Experimental Paraasitology, 41, 242–251.CrossRefGoogle Scholar
Befus, D. & Bienenstock, J. (1982) Factors involved in symbiosis and host resistance at the mucosaparasite interface. Progress in Allergy, 31, 76–177.Google ScholarPubMed
Bindseil, E. & Christensen, N. Ø. (1984) Thymus-independent hyperplasia and villous atrophy in the small intestine of mice infected with the trematode Echinostoma revolutum. Parasitology, 88, 431–438.CrossRefGoogle ScholarPubMed
Christensen, N. Ø., Knudsen, J. & Andreassen, J. (1986) Echinostoma revolutum: resistance to secondary and superimposed infections in mice. Experimental Parasitology, 61, 311–318.CrossRefGoogle ScholarPubMed
Christensen, N. Ø., Odaibo, A. B. & Simonsen, P. E. (1988) Review article. Echinostoma population regulation in experimental rodent definitive hosts. Parasitology Research, 75, 83–87.CrossRefGoogle Scholar
Cypess, R. H., Ebersole, J. L. & Molinari, J. A. (1977) Specific antibody levels in the intestinal perfusates of Heligosomoides polygyrus-infected mice. International Archives for Allergy and Applied lmmunology, 55, 496–503.CrossRefGoogle ScholarPubMed
Harrison, L. J. S. & Parkhouse, R. M. E. (1986) Passive protection against Taenia Saginata infection in cattle by a mouse monoclonal antibody reactive with the surface of the invasive oncosphere. Parasite Immunology, 8, 319–332.CrossRefGoogle ScholarPubMed
Huffman, J. E. & Fried, B. (1990) Echinostoma and echinostomiasis. Advances in Parasitology, 29, 215–269.CrossRefGoogle ScholarPubMed
Kawanishi, H. (1989) Recent progress in immune responses in gut. Digestive Disorders, 7, 113–124.CrossRefGoogle ScholarPubMed
Odaibo, A. B., Christensen, N. Ø. & Ukoli, F. M. A. (1988) Establishment, survival and fecundity in Echinostoma caproni (Trematoda) infections in NMRI mice. Proceedings of the Helminthological Society of Washington, 55, 265–269.Google Scholar
Odaibo, A. B., Christensen, N. Ø. & Ukoli, F. M. A. (1989) Further studies on the population regulation in Echinostoma caproni infections in NMRI mice. Proceedings of the Helminthological Society of Washington, 56, 192–198.Google Scholar
Poulain, J., Luffau, G. & Pery, P. (1976) Nippostrongylus brasiliensis in the rat; Immune response in serum and intestinal secretions. Annales d'Immunologie (Institut Pasteur), 127, 215–224.Google ScholarPubMed
Roitt, I., Brostoff, J. & Male, D. (1985) Immunology. Churchill Livingstone.Google Scholar
Simonsen, P. E. & Andersen, B. J. (1986) Echinostoma revolutum in mice: Dynamics of the antibody attack to the surface of an intestinal trematode. International Journal for Parasitology, 16, 475–482.CrossRefGoogle Scholar
Simonsen, P. E., Bindseil, E. & KØIE, M. (1989) Echinostoma caproni in mice: Studies on the attachment site of an intestinal trematode. International Journal for Parasitology, 19, 561–566.CrossRefGoogle ScholarPubMed
Simonsen, P. E., Vennervald, B. J. & Birch-Andersen, A. (1990) Echinostoma caproni in mice: Ultrastructural studies on the formation of immune complexes on the surface of an intestinal trematode. International Journal for Parasitology, 20, 935–941.CrossRefGoogle ScholarPubMed
Simonsen, P. E., Estambale, B. B. & Agger, M. (1991) Antibodies in the serum of golden hamsters experimentally infected with the intestinal trematode Echinostoma caproni. Journal of Helninthology, 65, 239–247.CrossRefGoogle ScholarPubMed
Underdown, B. J. & Schiff, J. M. (1986) Immunoglobulin A: Strategic defense initiative at the mucosal surface. Annual Reviews in Immunology, 4, 389–417.CrossRefGoogle ScholarPubMed
Van Der Vorst, E., Dhont, H., Van Haeren, C., Deceunynck, R. & Rycke, P. H. (1989) Hymenolepis murissylvatici: humoral response in intestinal lavages of the mouse. Journal of Helminthology, 63, 25–31.CrossRefGoogle ScholarPubMed
Voller, A. & Savigny, D. (1981) Enzyme linked immunosorbent assay (ELISA). In: Techniques in Clinical Immunology (editor Thompson, R. A) pp. 157–169. Blackwell Scientific Publications. Oxford.Google Scholar