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Angiostrongylus costaricensis egg antigen for the immunodiagnosis of abdominal angiostrongyliasis

Published online by Cambridge University Press:  01 September 2008

P. Mesén-Ramírez ,
E. Abrahams-Sandí ,
K. Fernández-Quesada  and
P. Morera
P. Mesén-Ramírez
Affiliation:
Department of Parasitology, University of Costa Rica, San Pedro/Mts. Oca, Costa Rica
E. Abrahams-Sandí*
Affiliation:
Department of Parasitology, University of Costa Rica, San Pedro/Mts. Oca, Costa Rica Pathology Service San Juan de Dios Hospital, San José, Costa Rica
K. Fernández-Quesada
Affiliation:
Microbiology Department, Boston Scientific Costa Rica, 302 Global Parkway, Heredia, Costa Rica
P. Morera
Affiliation:
Pathology Service San Juan de Dios Hospital, San José, Costa Rica
*
*Fax: +506-225-2374[email protected]
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Abstract

Angiostrongylus costaricensis is the aetiological agent of human abdominal angiostrongyliasis, a parasitic disease reported from the United States to Argentina, with a widespread occurrence of the nematode throughout Central and South America. This study assesses the performance of A. costaricensis eggs as antigen in an enzyme-linked immunosorbent assay (ELISA), for the determination of parasite-specific IgG1 antibodies. The specificity and the sensitivity of the method were 87% and 90.5%, respectively. Through this test it was possible to demonstrate a sharp and early decline in IgG1 antibody in serum samples taken from patients with histopathological diagnosis of abdominal angiostrongyliasis at different time points after surgical treatment. The present work demonstrated the usefulness of the egg antigen in the development of a specific diagnostic test for abdominal angiostrongylosis.

Type
Research Papers
Information
Journal of Helminthology , Volume 82 , Issue 3 , September 2008 , pp. 251 - 254
Copyright
Copyright © Cambridge University Press 2008

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