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An ELISA kit with two detection modes for the diagnosis of lymphatic filariasis

Published online by Cambridge University Press:  27 May 2014

S. Wongkamchai ,
W. Satimai ,
S. Loymek ,
H. Nochot  and
J.J. Boitano
S. Wongkamchai*
Affiliation:
Department of Parasitology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand
W. Satimai
Affiliation:
Bureau of Vector-Borne Disease, Department of Disease Control, Nonthaburi Province, Thailand
S. Loymek
Affiliation:
Filaria Project, Phikulthong Royal Development Study Center, Narathiwatt, Thailand
H. Nochot
Affiliation:
Department of Parasitology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand
J.J. Boitano
Affiliation:
Department of Parasitology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand
*
*Fax: +66-2-4112084 E-mail: [email protected]
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Abstract

The aim of this study was to develop a low-cost antifilarial immunoglobulin (Ig) G4 detection kit for the diagnosis of lymphatic filariasis. The kit was designed to be used by minimally trained personnel without the constraints of expensive laboratory equipment. We provide a description of the development and validation of a single-serum-dilution based enzyme-linked immunosorbent assay (ELISA) kit with ready-to-use reagents for measuring antifilarial IgG4 antibodies. The kit was tested on residents in Brugia malayi-endemic areas in southern Thailand. Detection was performed by naked-eye observation of the resultant colour of the immunological reactivity. The coefficient of variation (CV) was used to assess the reproducibility of the results. Long-term stability was measured over a 6-month period. Sensitivity of the test kit was 97% when compared with microfilariae detection in thick blood smears. Specificity was 98.7% based on the sera of 57 patients living outside the endemic areas who were infected with other parasites and 100 parasite-free subjects. All positive CVs were < 10%. The test kit was remarkably stable over 6 months. Field validation was performed by the detection of antifilarial IgG4 in 4365 serum samples collected from residents of brugian filariasis-endemic areas and compared with outcome colours of the test samples by the naked eye. Subsequent ELISA evaluation of these results using an ELISA reader indicated high agreement by the kappa statistic. These results demonstrate that the test kit is efficient and useful for public health laboratories as an alternative tool for the diagnosis of lymphatic filarial infection.

Type
Research Papers
Information
Journal of Helminthology , Volume 89 , Issue 5 , September 2015 , pp. 552 - 558
Copyright
Copyright © Cambridge University Press 2014 

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