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Adherence of carp leucocytes to adults and cercariae of the blood fluke Sanguinicola inermis

Published online by Cambridge University Press:  05 June 2009

D.T. Richards ,
D. Hoole ,
J.W. Lewis ,
E. Ewens  and
C. Arme
D.T. Richards
Affiliation:
Parasitology Research Laboratory, Centre for Applied Entomology & Parasitology, Department of Biological Sciences, Keele University, Keele, Staffordshire, ST5 5BG, UK
D. Hoole
Affiliation:
Parasitology Research Laboratory, Centre for Applied Entomology & Parasitology, Department of Biological Sciences, Keele University, Keele, Staffordshire, ST5 5BG, UK
J.W. Lewis
Affiliation:
School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, TW20 OEX, UK
E. Ewens
Affiliation:
School of Biological Sciences, Royal Holloway, University of London, Egham, Surrey, TW20 OEX, UK
C. Arme
Affiliation:
Parasitology Research Laboratory, Centre for Applied Entomology & Parasitology, Department of Biological Sciences, Keele University, Keele, Staffordshire, ST5 5BG, UK
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Abstract

Live adult and cercarial stages of Sanguinicola inermis Plehn, 1905 (Trematoda: Sanguinicolidae) were maintained in vitro in the presence of carp (Cyprinus carpio L.) leucocytes. Cells and parasites were fixed at intervals from 0.25 to 48 h and examined using light microscopy, SEM and TEM. Within 12 h of exposure, leucocytes were found attached to cercariae although, by 24 h, fewer cells were found attached to postcercarial, juvenile adult stages that had shed their tails. Neutrophils and macrophages were found attached to the damaged tegument of cercariae that had not transformed by 48 h. Few cells were attached to the tegument of adult flukes that were alive when fixed. However, there was extensive tegumental damage and numerous cells were attached to adult flukes that had died before fixation. The results are discussed with reference to parasite survival within the vascular system of the host.

Type
Research Papers
Information
Journal of Helminthology , Volume 70 , Issue 1 , March 1996 , pp. 63 - 67
Copyright
Copyright © Cambridge University Press 1996

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