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The utility of human filarial serum in the detection of circulating antigen

Published online by Cambridge University Press:  18 November 2009

P. Kaliraj ,
Indira Kharat ,
S. N. Ghirnikar  and
B. C. Harinath
P. Kaliraj
Affiliation:
Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha, India–442 102
Indira Kharat
Affiliation:
Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha, India–442 102
S. N. Ghirnikar
Affiliation:
Research cum Training Centre, Wardha
B. C. Harinath*
Affiliation:
Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha, India–442 102
*
†Address for correspondence: Prof. B. C. Marinath, Department of Biochemistry, M. G. Inst. of Med. Sciences, Sevagram, Wardha (dt), Maharashtra, India-442 102.
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Abstract

The utility of human filarial serum immunoglobulin (FSI) in detecting circulating antigen in filarial sera was studied by counter Immunoelectrophoresis (CIEP) and the indirect haemagglutination test (IHAT). CIEP was found to be better than IHAT. 23 out of 30 sera from persons with microfilaraemia and one of 30 clinical cases of filariasis, but none of the normal sera or sera from those with helminths other than filariae, showed the presence of circulating filarial antigen in CIEP. FSI was fractionated by DEAE- Sephadex A-50 column chromatography and the antibody active in CIEP was found to be IgG in nature.

Type
Research Article
Information
Journal of Helminthology , Volume 55 , Issue 2 , June 1981 , pp. 133 - 140
Copyright
Copyright © Cambridge University Press 1981

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