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Steinernema innovationi n. sp. (Panagrolaimomorpha: Steinernematidae), a new entomopathogenic nematode species from South Africa

Published online by Cambridge University Press:  03 April 2014

H. Çimen ,
M.-M. Lee ,
J. Hatting ,
S. Hazir  and
S.P. Stock
H. Çimen
Affiliation:
Adnan Menderes University, Faculty of Arts and Science, Department of Biology, 09010Aydin, Turkey
M.-M. Lee
Affiliation:
Department of Entomology, University of Arizona, 1140 E. South Campus Dr., Tucson, AZ 85721-0036, Arizona, USA
J. Hatting
Affiliation:
South African Agricultural Research Council, Small Grain Institute, Private Bag X29, Bethlehem, 9701, South Africa
S. Hazir
Affiliation:
Adnan Menderes University, Faculty of Arts and Science, Department of Biology, 09010Aydin, Turkey
S.P. Stock*
Affiliation:
Department of Entomology, University of Arizona, 1140 E. South Campus Dr., Tucson, AZ 85721-0036, Arizona, USA
*
*Fax: +1-520-621-1150 E-mail: [email protected]
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Abstract

Morphological and molecular sequence data were combined with cross-hybridization studies and used to identify a new Steinernema sp. from Free State, South Africa. Molecular and morphological data indicate that the new species belongs to the ‘glaseri-group’ of Steinernema spp. Key morphological diagnostic characters for S. innovationi n. sp. include the morphometric features of the third-stage infective juveniles: total body length = 1054 (1000–1103) μm, tail length = 108 (97–117) μm, location of the excretory pore = 88 (82–91) μm, and D% = 58 (54–63), E% = 115 (104–137) and H% = 43 (37–46). Additionally, the morphology of the spicules and gubernaculum of the first-generation males are considered key diagnostic traits. Steinernema innovationi n. sp. was also characterized by analysis of both rDNA and mitochondrial gene sequence data, which further indicate the uniqueness of this Steinernema species.

Type
Research Papers
Information
Journal of Helminthology , Volume 89 , Issue 4 , July 2015 , pp. 415 - 427
Copyright
Copyright © Cambridge University Press 2014 

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References

Artyukhovsky, A.K., Kozodoi, E.M., Reid, A.P. & Spiridonov, S.E. (1997) Redescription of Steinernema arenarium (Artyukhovsky, 1967) topotype from Central Russia and a proposal for S. anomalae (Kozodoi, 1984) as a junior synonym. Russian Journal of Nematology 5, 31–37.Google Scholar
Bedding, R.A. & Akhurst, R.J. (1975) A simple technique for the detection of insect parasitic rhabditid nematodes in soil. Nematologica 21, 109–110.CrossRefGoogle Scholar
Clausi, M., Longo, G., Rappazzo, G., Tarasco, E. & Vinciguerra, M.T. (2011) Steinernema vulcanicum n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode species from Sicily (Italy). Nematology 13, 409–423.Google Scholar
De Waal, J.Y., Malan, A.P., Levings, J. & Addison, M.F. (2010) Key elements in the successful control of diapausing codling moth, Cydia pomonella (Lepidoptera: Tortricidae) in wooden fruit bins with a South African isolate of Heterorhabditis zealandica (Rhabditida: Heterorhabditidae). Biocontrol Science and Technology 20, 489–502.CrossRefGoogle Scholar
Edington, S., Buddi, A.G., Tymo, L., Hunt, D.J., Nguyen, K.B., France, A.I., Merino, L.M. & Moore, D. (2009) Steinernema australe n. sp. (Panagrolaimomorpha: Steinernematidae), a new entomopathogenic nematode from Isla Magdalena, Chile. Nematology 11, 699–717.CrossRefGoogle Scholar
Franklin, M. & Goodey, J.B. (1949) A cotton blue–lactophenol method for mounting plant parasitic nematodes. Journal of Helminthology 23, 175–178.CrossRefGoogle Scholar
Hatting, J., Stock, S.P. & Hazir, S. (2009) Diversity and distribution of entomopathogenic nematodes (Steinernematidae, Heterorhabditidae) in South Africa. Journal of Invertebrate Pathology 102, 120–128.CrossRefGoogle ScholarPubMed
Hominick, W.M., Briscoe, B.R., del Pino, F.G., Heng, J., Hunt, D.J., Kozodoy, E., Mráček, Z., Nguyen, K.B., Reid, A.P., Spiridonov, S., Stock, S.P., Sturhan, D., Waturu, C. & Yoshida, M. (1997) Biosystematics of entomopathogenic nematodes, current status, protocols and definitions. Journal of Helminthology 71, 271–298.CrossRefGoogle ScholarPubMed
Kaya, H.K. & Stock, S.P. (1997) Techniques in insect nematology. pp. 281–324in Lacey, L. (Ed.) Manual of techniques in insect pathology. San Diego, California, Academic Press.CrossRefGoogle Scholar
Khatri-Chhetri, H.B., Waeyenberge, L., Spiridonov, S., Manandhar, H.K. & Moens, M. (2011) Steinernema lamjungense n. sp. (Rhabditida: Steinernematidae), a new species of entomopathogenic nematode from Lamjung district, Nepal. Nematology 13, 589–605.CrossRefGoogle Scholar
Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I.M., Wilm, A., Lopez, R., Thompson, J.D., Gibson, T.J. & Higgins, D.G. (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23, 2947–2948.CrossRefGoogle ScholarPubMed
Lee, M.M., Sicard, M., Skeie, M. & Stock, S.P. (2009) Steinernema boemarei n. sp. (Nematoda: Steinernematidae), a new entomopathogenic nematode from southern France. Systematic Parasitology 72, 127–141.CrossRefGoogle Scholar
Maddison, W.P. & Maddison, D.R. (2011) Mesquite: a modular system for evolutionary analysis. Version 2.75http://mesquiteproject.org.Google Scholar
Malan, A.P. & Manrakhan, A. (2009) Susceptibility of the Mediterranean fruit fly (Ceratitis capitata) and the Natal fruit fly (Ceratitis rosa) to entomopathogenic nematodes. Journal of Invertebrate Pathology 100, 47–49.CrossRefGoogle Scholar
Malan, A.P., Nguyen, K.B. & Addison, M.F. (2006) Entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) from the southwestern parts of South Africa. African Plant Protection 12, 65–69.Google Scholar
Malan, A.P., Nguyen, K.B., De Waal, J.Y. & Tiedt, L. (2008) Heterorhabditis safricana n. sp. (Rhabditida: Heterorhabditidae), a new entomopathogenic nematode from South Africa. Nematology 10, 381–396.Google Scholar
Malan, A.P., Knoetze, R. & Moore, S.D. (2011) Isolation and identification of entomopathogenic nematodes from citrus orchards in South Africa and their biocontrol potential against false codling moth. Journal of Invertebrate Pathology 108, 115–125.CrossRefGoogle ScholarPubMed
Malan, A.P., Knoetze, R. & Tiedt, L. (2012) Heterorhabditis noenieputensis n. sp. (Rhabditida: Heterorhabditidae), a new entomopathogenic nematode from South Africa. Journal of Helminthology 12, 1–13.Google Scholar
McClure, M.J. & Stowell, L.J. (1978) A simple method of processing nematodes for electron microscopy. Journal of Nematology 18, 376–377.Google Scholar
Mráček, Z., Hernandez, E.A. & Boemare, N.E. (1994) Steinernema cubana sp. n. (Nematoda: Rhabditida: Steinernematidae) and the preliminary characterization of its associated bacterium. Journal of Invertebrate Pathology 64, 123–129.CrossRefGoogle Scholar
Nadler, S.A., Bolotin, E. & Stock, S.P. (2006) Phylogenetic relationships of Steinernema (Cephalobina, Steinernematidae) based on nuclear, mitochondrial, and morphological data. Systematic Parasitology 63, 159–179.CrossRefGoogle ScholarPubMed
Nguyen, K.B. & Buss, E.A. (2011) Steinernema phyllophagae n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Florida, USA. Nematology 13, 425–442.Google Scholar
Nguyen, K.B. & Duncan, L.W. (2002) Steinernema diaprepesi n. sp. (Rhabditida: Steinernematidae), a parasite of the citrus root weevil Diaprepes abbreviatus (L.) (Coleoptera: Curculionidae). Journal of Nematology 34, 159–170.Google Scholar
Nguyen, K.B., Maruniak, J. & Adams, B.J. (2001) The diagnostic and phylogenetic utility of the rDNA internal transcribed spacer sequences in Steinernema. Journal of Nematology 33, 73–82.Google ScholarPubMed
Nguyen, K.B, Malan, A & Gozel, U (2006) Steinernema khoisanae n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from South Africa. Nematology 8, 157–175.Google Scholar
Nguyen, K.B., Inarte, C.M.A., Leite, L.G., Santos, J.M.D. & Harakava, R. (2010) Steinernema brazilense n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Mato Grosso, Brazil. Journal of Invertebrate Pathology 103, 8–20.CrossRefGoogle Scholar
Poinar, G.O. Jr (1990) Entomopathogenic nematodes in biological control. pp. 23–74in Gaugler, R. & Kaya, K.H. (Eds) Taxonomy and biology of Steinernematidae and Heterorhabditidae. Boca Raton, Florida, CRC Press.Google Scholar
Qiu, L., Van, X., Zhou, Y., Nguyen, K.B. & Pang, Y. (2005) Steinernema aciari sp. n. (Nematoda: Steinernematidae), a new entomopathogenic nematode from Guangdong, China. Journal of Invertebrate Pathology 88, 58–69.CrossRefGoogle Scholar
Qiu, Y.F., Fang, Y., Zhou, Y., Pang, Y. & Nguyen, K.B. (2004) Steinernema guangdongense sp. n. (Nematoda: Steinernematidae), a new entomopathogenic nematode from southern China with a note on S. serratum (nomen nudum). Zootaxa 704, 1–20.CrossRefGoogle Scholar
Román, J. & Figueroa, W. (1994) Steinernema puertoricensis n. sp. (Rhabditida: Steinernematidae) a new entomopathogenic nematode from Puerto Rico. Journal of Agriculture, University of Puerto Rico 78, 167–175.CrossRefGoogle Scholar
Seinhorst, J.W. (1959) A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica 4, 67–69.CrossRefGoogle Scholar
Spaull, V.W. (1988) A preliminary evaluation of entomopathogenic nematodes to control the African sugarcane stalk borer Eldana saccharina (Lepidoptera: Pyralidae). Proceedings of the South African Sugar Technology Association 62, 120–123.Google Scholar
Spaull, V.W. (1990) Field tests to control the pyralid, Eldana saccharina, with an entomogenous nematode, Heterorhabditis sp. Proceedings of the South African Sugar Technology Association 64, 103–106.Google Scholar
Spaull, V.W. (1991) Heterorhabditis and Steinernema species (Nematoda: Rhabditida) for the control of a sugar cane stalk borer in South Africa. Phytophylactica 23, 213–215.Google Scholar
Spiridinov, S.E., Reid, A.P., Podrucka, K., Subbotin, S.A. & Moens, M. (2004) Phylogenetic relationships within the genus Steinernema (Nematoda: Rhabditida) as inferred from analysis of sequences of the ITS-1–5.8S–ITS2 region of rDNA and morphological features. Nematology 6, 547–566.CrossRefGoogle Scholar
Steiner, G. (1929) Neoaplectana glaseri n. g. n. sp. (Oxyuridae), a new nemic parasite of the Japanese beetle (Popillia japonica Newm.). Journal of the Washington Academy of Science 19, 436–440.Google Scholar
Stock, S.P. & Goodrich-Blair, H. (2012) Nematode parasites, pathogens and associates of insects and invertebrates of economic importance. pp. 375–425in Lacey, L.A. (Ed.) Manual of techniques in invertebrate pathology. Yakima, Washington, Elsevier.Google Scholar
Stock, S.P. & Koppenhöfer, A.M. (2003) Steinernema scarabaei n. sp. (Rhabditida: Steinernematidae), a natural pathogen of scarab beetle larvae (Coleoptera: Scarabaeidae) from New Jersey, USA. Nematology 5, 191–204.Google Scholar
Stock, S.P., Campbell, J.F. & Nadler, S.A. (2001a) Phylogeny of Steinernema Travassos, 1927 (Cephalobina, Steinernematidae) inferred from ribosomal DNA sequences and morphological characters. Journal of Parasitology 87, 877–889.CrossRefGoogle ScholarPubMed
Stock, S.P., Heng, J., Hunt, D.J., Reid, A.P., Shen, X. & Choo, H.Y. (2001b) Redescription of Steinernema longicaudum Shen & Wang (Nematoda: Steinernematidae); geographic distribution and phenotypic variation between allopatric populations. Journal of Helminthology 75, 81–92.CrossRefGoogle ScholarPubMed
Stock, S.P., Griffin, C.T. & Chaenari, R. (2004) Morphological and molecular characterization of Steinernema hermaphroditum n. sp. (Nematoda: Steinernematidae), an entomopathogenic nematode from Indonesia, and its phylogenetic relationships with other members of the genus. Nematology 6, 401–412.CrossRefGoogle Scholar
Stokwe, N., Malan, A., Nguyen, K., Knoetze, R. & Tiedt, L. (2011) Steinernema citrae n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from South Africa. Nematology 13, 569–587.CrossRefGoogle Scholar
Swofford, D.L. (2002) PAUP*. Phylogenetic analysis using parsimony (and other methods), version 4. Sunderland, Massachusetts, Sinauer Associates.Google Scholar
Tamiru, T., Waeyenberge, L., Hailu, T., Ehlers, R.U., Puza, V. & Mracek, Z. (2012) Steinernema ethiopiense sp. n. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Ethiopia. Nematology 14, 741–757.CrossRefGoogle Scholar
Uribe-Lorío, L., Mora, M. & Stock, S.P. (2007) Steinernema costaricense n. sp. and Steinernema puntauvense n. sp. (Rhabditida, Steinernematidae), two new entomopathogenic nematodes from Costa Rica. Systematic Parasitology 68, 167–172.CrossRefGoogle Scholar
Van Niekerk, S. & Malan, A.P. (2012) Potential of South African entomopathogenic nematodes (Heterorhabditidae and Steinernematidae) for control of the citrus mealybug, Planococcus citri (Pseudococcidae). Journal of Invertebrate Pathology 11, 166–174.CrossRefGoogle Scholar