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Serological and coprological analyses for the diagnosis of Fasciolagigantica infections in bovine hosts from Sargodha, Pakistan

Published online by Cambridge University Press:  24 August 2015

T. Rehman ,
M.N. Khan ,
R.Z. Abbas ,
W. Babar ,
A. Sikandar  and
M.A. Zaman
T. Rehman*
Affiliation:
UCV&AS, The Islamia University of Bahawalpur, Pakistan
M.N. Khan
Affiliation:
Department of Parasitology, University of Agriculture, Faisalabad, Pakistan
R.Z. Abbas
Affiliation:
UCV&AS, The Islamia University of Bahawalpur, Pakistan
W. Babar
Affiliation:
UCV&AS, The Islamia University of Bahawalpur, Pakistan
A. Sikandar
Affiliation:
Department of Anatomy and Histology, CV&AS, Jhang, Pakistan
M.A. Zaman
Affiliation:
Department of Parasitology, CV&AS, Jhang, Pakistan
*
* E-mail: [email protected]
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Abstract

A serological and coprological survey of fasciolosis was conducted in bovine hosts from the Sargodha district, Pakistan using excretory–secretory (ES) antigens of Fasciola gigantica from cattle and buffaloes. Livers, faecal and blood samples of 146 cattle and 184 buffaloes were collected from slaughterhouses and examined for the presence of any Fasciola in bile ducts and ova in faeces. Serum was separated. ES antigens were prepared by incubating adult Fasciola in phosphate-buffered saline for 6–8 h and then filtering using a 0.22-μm syringe filter. Checkerboard titration was performed and optimum concentrations of antigen and serum were determined. Sero-prevalence was found to be 50.00 and 38.35% in buffalo and cattle, respectively. Using liver examination as the gold standard, enzyme-linked immunosorbent assay (ELISA) sensitivity was found to be 100% in both buffalo and cattle as compared with that of coprological examination in buffalo (61.79%) and cattle (54.54%). This indigenous ELISA was also highly specific, with values of 96.84 and 98.90% in buffalo and cattle, respectively. Positive predictive values were calculated as 96.74 and 98.21% in buffalo and cattle, respectively, while negative predictive values were 100%. For the validation of indigenous ELISA in field surveys, faecal and blood samples were collected from six sub-districts (tehsils) in the district of Sargodha. Sera were screened for the presence of anti-fasciola antibodies using both the indigenous and commercial ELISA kits. While both kits were equally sensitive, the indigenous ELISA was found to be more specific. The highest prevalence of fasciolosis was found in December, as ascertained using both serological and coprological examination. Significant differences were found in prevalences of fasciolosis in different sub-districts and age groups, together with feeding and watering systems.

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 4 , July 2016 , pp. 494 - 502
Copyright
Copyright © Cambridge University Press 2015 

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References

Alves, D.P., Carneiro, M.B., Martins, I.V.F., Bernardo, C.C., Donatele, D.M., Pereira, O.S. Jr, Almeida, B.R., Avelar, B.R. & Leão, A.G.C. (2011) Distribution and factors associated with Fasciola hepatica infection in cattle in the south of Espírito Santo State, Brazil. Journal of Venomous Animals and Toxins including Tropical Diseases 3, 271–276.Google Scholar
Anderson, N., Luong, T.T., Vo, N.G., Bui, K.L., Smooker, P.M. & Spithill, T.W. (1999) The sensitivity and specificity of two methods for detecting Fasciola infections in cattle. Veterinary Parasitology 83, 15–24.CrossRefGoogle ScholarPubMed
Asrat, M. (2004) Infection prevalence of ovine fasciolosis in irrigation schemes along the Upper Awash River Basin and effects of strategic anthelmintic treatment in selected upstream areas. MSc Thesis, Addis Ababa University, Ethiopia.Google Scholar
Awad, W.S., Ibrahim, A.K. & Salib, F.A. (2009) Using indirect ELISA to assess different antigens for the serodiagnosis of Fasciola gigantica infection in cattle, sheep and donkeys. Research in Veterinary Science 86, 466–471.Google Scholar
Boray, J.C. (1967) The effect of host reaction to experimental Fasciola hepatica infections in sheep and cattle. pp. 84–96 in Soulsby, E.J.L. (Ed.) The reaction of the host to parasitism. Marburg, Germany, Elwert.Google Scholar
Cabán-Hernández, K., Gaudier, J.F., Ruiz-Jiménez, C. & Espino, A.M. (2014) Development of two antibody detection enzyme-linked immunosorbent assays for serodiagnosis of human chronic fasciolosis. Journal of Clinical Microbiology 52, 766–772.Google Scholar
Charlier, J., Meulemeester, L.D., Claerebout, E., Williams, D. & Vercruysse, J. (2008) Qualitative and quantitative evaluation of coprological and serological techniques for the diagnosis of fasciolosis in cattle. Veterinary Parasitology 153, 44–51.Google Scholar
Charlier, J., Vercruysse, J., Morgan, E., Dijk, J.V. & Williams, D.J.L. (2014) Recent advances in the diagnosis, impact on production and prediction of Fasciola hepatica in cattle. Parasitology 141, 326–335.Google Scholar
Cornelissen, J.B., Gaasenbeek, C.P., Borgsteede, F.H., Holland, W.G., Harmsen, M.M. & Boersma, W.J. (2001) Early immunodiagnosis of fasciolosis in ruminants using recombinant Fasciola hepatica cathepsin L-like protease. International Journal of Parasitology 31, 728–737.Google Scholar
Cruz-Mendoza, I., Ibarra-Velarde, F., Quintero-Martínez, M.T., Naranjo-García, E., Lecumberri-López, J. & Correa, D. (2005) Seasonal transmission of Fasciola hepatica in cattle and Lymnaea (Fossaria) humilis snails in central Mexico. Parasitology Research 95, 283–286.CrossRefGoogle ScholarPubMed
Demerdash, Z.A., Diab, T.M., Aly, I.R., Mohamed, S.H., Mahmoud, F.S., Zoheiry, M.K., Mansour, W.A., Attia, M.E. & El-Bassiouny, A.E. (2011) Diagnostic efficacy of monoclonal antibody based sandwich enzyme linked immunosorbent assay (ELISA) for detection of Fasciola gigantica excretory/secretory antigens in both serum and stool. Parasites & Vectors 4, 176–182.Google Scholar
Doyle, J.J. (1971) Acquired immunity to experimental infection with Fasciola hepatica in cattle. Research in Veterinary Science 12, 527–534.Google Scholar
Estuningsih, E., Spithill, T., Raadsma, H., Law, R., Adiwinata, G., Meeusen, E. & Piedrafita, D. (2009) Development and application of a faecal antigen diagnostic sandwich ELISA for estimating prevalence of Fasciola gigantica in cattle in central Java. Indonesian Journal of Parasitology 95, 450–455.CrossRefGoogle ScholarPubMed
Fagbemi, B.O. & Guobadia, E.E. (1995) Immunodiagnosis of fasciolosis in ruminants using a 28-kDa cysteine protease of Fasciola gigantica adult worms. Veterinary Parasitology 57, 309–318.CrossRefGoogle ScholarPubMed
Fagbemi, B.O. & Obarisiagbon, I.O. (1991) Common antigens of Fasciola gigantica, Dicrocoelium hospes and Schistosoma bovis and their relevance to serology. Veterinary Quarterly 13, 81–87.Google Scholar
Fatima, M., Chishti, M.Z., Ahmad, F. & Lone, B.A. (2012) Epidemiological study of fasciolosis in cattle of Kashmir Valley. Advances in Biological Research 6, 106–109.Google Scholar
Greiner, M. & Gardner, I.A. (2000) Epidemiologic issues in the validation of veterinary diagnostic tests. Preventive Veterinary Medicine 45, 3–22.CrossRefGoogle ScholarPubMed
Hamad, K.K., Iqbal, Z., Sindhu, Z.D. & Muhammad, G. (2013) Antinematicidal activity of Nicotiana tabacum L. leaf extracts to control benzimidazole-resistant Haemonchus contortus in sheep. Pakistan Veterinary Journal 33, 85–90.Google Scholar
Hamad, K.K., Iqbal, Z., Sindhu, Z.U.D., Abbas, R.Z., Khan, A., Muhammad, G. & Epperson, B. (2014) Combination of Nicotiana tabacum and Azadirachta indica: A novel substitute to control levamisole and ivermectin resistant Haemonchus contortus in ovines. Pakistan Veterinary Journal 34, 24–29.Google Scholar
Hillyer, G.V., de Galanes, M.S., Buchon, P. & Bjorland, J. (1996) Herd evaluation by enzyme-linked immunosorbent assay for the determination of Fasciola hepatica infection in sheep and cattle from the Altiplano of Bolivia. Veterinary Parasitology 61, 211–220.Google Scholar
Ibarra, F.A., Montenegro, N., Vera, Y., Boulard, C., Quiroz, H., Flores, J. & Ochoa, P. (1998) Comparison of three ELISA tests for seroepidemiology of bovine fascioliosis. Veterinary Parasitology 77, 229–236.Google Scholar
Jalali, M.H.R., Zadeh, S.E., Abadi, M.G.N. & Yamchi, J.A. (2012) Liver histopathology and an in-house indirect ELISA method for the diagnosis of bovine fasciolosis. Comparative Clinical Pathology 21, 1623–1627.Google Scholar
Khan, M.K., Sajid, M.S., Khan, M.N., Iqbal, Z. & Iqbal, M.U. (2009) Bovine fasciolosis: prevalence, effects of treatment on productivity and cost benefit analysis in five districts of Punjab, Pakistan. Research Veterinary Science 87, 70–75.CrossRefGoogle ScholarPubMed
Lauridsen, J. (2005) Design and analysis of samples, Module 6: Systematic and stratified sampling. Available at http://statmaster.sdu.dk/courses/st118 (accessed accessed March 2011).Google Scholar
Liu, G.H., Gasser, R.B., Young, N.D., Song, H.Q., Ai, L. & Zhu, X.Q. (2014) Complete mitochondrial genomes of the ‘intermediate form’ of Fasciola and Fasciola gigantica, and their comparison with F. hepatica . Parasites & Vectors 7, 150–160.Google Scholar
Maqbool, A., Hayat, C.S., Akhtar, T. & Hashmi, H.A. (2002) Epidemiology of fasciolosis in buffaloes under different management conditions. Veterinarski Archive 72, 221–228.Google Scholar
Mezo, M., González-Warleta, M., Carro, C. & Ubeira, F.M. (2004) An ultrasensitive capture ELISA for detection of Fasciola hepatica coproantigens in sheep and cattle using a new monoclonal antibody (MM3). Journal of Parasitology 90, 845–852.Google Scholar
Mufti, S., Afshan, K., Khan, I.A., Zafar, Y., Rizvi, S.S.R., Nazir, F. & Qayyum, M. (2014) Genetic characterization of Fasciola samples from bovine hosts in Pakistan by sequences of ribosomal internal transcribed spacer regions. Pakistan Veterinary Journal 34, 361–366.Google Scholar
Mulcahy, G., Joyce, P. & Dalton, J.P. (1999) Immunology of Fasciola hepatica infection. pp. 341–375 in Dalton, J.P. (Ed.) Fasciolosis. Wallingford, CAB International.Google Scholar
Ortiz, P.L., Claxton, J.R., Clarkson, M.J., McGarry, J. & Williams, D.J.L. (2000) The specificity of antibody responses in cattle naturally exposed to Fasciola hepatica . Veterinary Parasitology 93, 121–134.CrossRefGoogle ScholarPubMed
Pfukenyi, D.M., Mukaratirwa, S., Willingham, A.L. & Monrad, J. (2006) Epidemiological studies of Fasciola gigantica infections in cattle in the highveld and lowveld communal grazing areas of Zimbabwe. Onderstepoort Journal of Veterinary Research 73, 37–51.Google Scholar
Phiri, A.M., Phiri, I.K., Sikasunge, C.S. & Monrad, J. (2005) Prevalence of fasciolosis in Zambian cattle observed at selected abattoirs with emphasis on age, sex and origin. Journal of Veterinary Medicine B 52, 414–416.Google Scholar
Rapsch, C., Schweizer, V., Grimm, F., Kohler, L., Bauer, C., Deplazes, P., Braun, U. & Torgerson, P.R. (2006) Estimating the true prevalence of Fasciola hepatica in cattle slaughtered in Switzerland in the absence of an absolute diagnostic test. International Journal of Parasitology 36, 1153–1158.Google Scholar
Ross, J.G. (1967) Studies of immunity to Fasciola hepatica. Acquired immunity in cattle, sheep and rabbits following natural infection and vaccine procedures. Journal of Helminthology 41, 393–399.Google Scholar
Salimi-Bejestani, M.R., McGarry, J.W., Felstead, S., Ortiz, P., Akca, A. & Williams, D.J.L. (2005) Development of an antibody-detection ELISA for Fasciola hepatica and its evaluation against a commercially available test. Research in Veterinary Science 78, 177–181.CrossRefGoogle ScholarPubMed
Santiago, N. & Hillyer, G.V. (1988) Antibody profiles by EITB and ELISA of cattle and sheep infected with Fasciola hepatica . Journal of Parasitology 74, 10–18.Google Scholar
SAS. (1998) SAS/STAT user guides. Version 6.12. Cary, North Carolina, SAS Institute Inc.Google Scholar
Soulsby, E.J.L. (2006) Helminths, arthropods and Protozoa of domesticated animals. 7th edn. London, Ballière Tindall.Google Scholar
Thrusfield, M. (2008) Veterinary epidemiology. 3rd edn. London, Blackwell.Google Scholar
Tsegaye, E., Begna, F. & Mulugeta, S. (2011) Prevalence of bovine fasciolosis and its economic significance in and around Assela, Ethiopia. Global Journal of Medical Research 11, 1–8.Google Scholar
Valeroa, M.A., Ubeira, F.M., Khoubbane, M., Artigas, P., Muino, L., Mezo, M., Pérez-Crespo, I., Periagoa, M.V. & Mas-Coma, S. (2009) MM3-ELISA evaluation of coproantigen release and serum antibody production in sheep experimentally infected with Fasciola hepatica and F. gigantica . Veterinary Parasitology 159, 77–81.Google Scholar
Varghese, A., Raina, O.K., Nagar, G., Garg, R., Banerjee, P.S., Maharana, B.R. & Kollannur, J.D. (2012) Development of cathepsin-L cysteine proteinase based Dot-enzyme-linked immunosorbent assay for the diagnosis of Fasciola gigantica infection in buffaloes. Veterinary Parasitology 183, 382–385.Google Scholar
Welch, D., Smith, P., Malone, J., Holmes, R. & Geagan, J. (1987) Herd evaluation of Fasciola hepatica infection level in Louisiana cattle by an enzyme-linked immunosorbent assay. American Journal of Veterinary Research 48, 345–347.Google Scholar
Yadav, S.C., Saini, M., Raina, E.O.K., Nambi, E.P.A., Jadav, K. & Sriveny, E.D. (2005) Fasciola gigantica cathepsin-L cysteine proteinase in the detection of early experimental fasciolosis in ruminants. Parasitology Research 97, 527–534.CrossRefGoogle ScholarPubMed