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Sequence variability in internal transcribed spacers of nuclear ribosomal DNA among isolates of the oxyurid nematodes Syphacia obvelata and Aspiculuris tetraptera from mice reared in laboratories in China

Published online by Cambridge University Press:  09 January 2015

J.H. Qiu ,
Y. Lou ,
Y. Zhang ,
Q.C. Chang ,
Z.X. Liu ,
H. Duan ,
D.H. Guo ,
D.Z. Gao ,
D.M. Yue  and
C.R. Wang
J.H. Qiu
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province163319, PR China
Y. Lou
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province163319, PR China
Y. Zhang
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province163319, PR China
Q.C. Chang
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province163319, PR China
Z.X. Liu
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province163319, PR China
H. Duan
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province163319, PR China
D.H. Guo
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province163319, PR China
D.Z. Gao
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province163319, PR China
D.M. Yue
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province163319, PR China
C.R. Wang*
Affiliation:
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province163319, PR China
*
*Fax: +86 (459) 6819095 E-mail: [email protected]
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Abstract

This study examined sequence variability in internal transcribed spacers (ITS) of nuclear ribosomal DNA among Syphacia obvelata and Aspiculuris tetraptera isolates from laboratory mice from different geographical locations in China. ITS1, 5.8S and ITS2 rDNA were amplified separately from adult S. obvelata and A. tetraptera individuals by polymerase chain reaction (PCR), and the amplicons were subjected to sequencing from both directions. The lengths of the sequences of ITS1, 5.8S and ITS2 rDNA from both nematodes were 314 bp and 456 bp, 157 bp, and 273 bp and 419 bp, respectively. The intraspecific sequence variations in S. obvelata ITS1 were 0–0.3%. For A. tetraptera they were 0–0.7% in ITS1 and 0–1.0% in ITS2. However, the interspecific sequence differences among members of the infraorder Oxyuridomorpha were significantly higher, being 54.0–65.5% for ITS1 and 55.3–64.1% for ITS2. Phylogenetic analysis based on the combined partial sequences of ITS1 and ITS2 using three inference methods – Bayesian inference, maximum likelihood and maximum parsimony – revealed that all the S. obvelata and A. tetraptera samples formed independent monophyletic groups. Syphacia obvelata was closer to Syphacia muris than to A. tetraptera, consistent with morphological classification. These results demonstrate that ITS1 and ITS2 rDNA sequences are useful markers for population genetic studies of oxyurid nematodes.

Type
Research Papers
Information
Journal of Helminthology , Volume 90 , Issue 1 , January 2016 , pp. 81 - 85
Copyright
Copyright © Cambridge University Press 2015 

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References

Baker, D.G. (1998) Natural pathogens of laboratory mice, rats, and rabbits and their effects on research. Clinical Microbiology Reviews 11, 231–266.CrossRefGoogle ScholarPubMed
Bugarski, D., Jovcic, G., Katic-Radivojevic, S., Petakov, M., Krstić, A., Stojanović, N. & Milenković, P. (2006) Hematopoietic changes and altered reactivity to IL-17 in Syphacia obvelata infected mice. Parasitology International 55, 91–97.CrossRefGoogle ScholarPubMed
Burland, T.G. (2000) DNASTAR's Lasergene sequence analysis software. Methods in Molecular Biology 132, 71–91.Google ScholarPubMed
Chen, X.M., Li, X., Lin, R.Q., Deng, J.Y., Fan, W.Y., Yuan, Z.G., Song, H.Q., Liao, M. & Zhu, X.Q. (2011) Pinworm infection in laboratory mice in southern China. Laboratory Animals 45, 58–60.CrossRefGoogle ScholarPubMed
Dai, R.S., Liu, G.H., Song, H.Q., Lin, R.Q., Yuan, Z.G., Li, M.W., Huang, S.Y., Liu, W. & Zhu, X.Q. (2012) Sequence variability in two mitochondrial DNA regions and internal transcribed spacer among three cestodes infecting animals and humans from China. Journal of Helminthology 86, 245–251.CrossRefGoogle ScholarPubMed
Gaherwal, S., Solanki, S., Prakash, M.M. & Wast, N. (2012) Aspiculuris tetraptera induced hematological parameters in infected and vaccinated mice. Iranian Journal of Parasitology 7, 61–66.Google ScholarPubMed
Hill, W.A., Randolph, M.M. & Mandrell, T.D. (2009) Sensitivity of perianal tape impressions to diagnose pinworm (Syphacia spp.) infections in rats (Rattus norvegicus) and mice (Mus musculus). Journal of the American Association for Laboratory Animal Science 48, 378–380.Google ScholarPubMed
Liu, G.H., Zhou, W., Nisbet, A.J., Xu, M.J., Zhou, D.H., Zhao, G.H., Wang, S.K., Song, H.Q., Lin, R.Q. & Zhu, X.Q. (2014) Characterization of Trichuris trichiura from humans and T. suis from pigs in China using internal transcribed spacers of nuclear ribosomal DNA. Journal of Helminthology 88, 64–68.CrossRefGoogle Scholar
Lou, Y., Zhang, Y., Qiu, J.H., Gao, J.F., Wang, W.T., Xiao, J.Y., Chang, Q.C. & Wang, C.R. (2014) Sequence variability in four mitochondrial genes among pinworm Aspiculuris tetraptera isolates from laboratory mice in four provinces in China. Mitochondrial DNA [Epub ahead of print].Google Scholar
Michels, C., Goyal, P., Nieuwenhuizen, N. & Brombacher, F. (2006) Infection with Syphacia obvelata (pinworm) induces protective Th2 immune responses and influences ovalbumin-induced allergic reactions. Infection and Immunity 74, 5926–5932.CrossRefGoogle ScholarPubMed
Okamoto, M., Urushima, H., Iwasa, M. & Hasegawa, H. (2007) Phylogenetic relationships of rodent pinworms (genus Syphacia) in Japan inferred from mitochondrial CO1 gene sequences. Journal of Veterinary Medical Science 69, 545–547.CrossRefGoogle ScholarPubMed
Okamoto, M., Urushima, H. & Hasegawa, H. (2009) Phylogenetic relationships of rodent pinworms (genus Syphacia) in Japan inferred from 28S rDNA sequences. Parasitology International 58, 330–333.CrossRefGoogle ScholarPubMed
Page, R.D. (1996) TREEVIEW: an application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12, 357–358.Google ScholarPubMed
Parel, J.D., Galula, J.U. & Ooi, H.K. (2008) Characterization of rDNA sequences from Syphacia obvelata, Syphacia muris, and Aspiculuris tetraptera and development of a PCR-based method for identification. Veterinary Parasitology 153, 379–383.CrossRefGoogle ScholarPubMed
Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572–1574.CrossRefGoogle ScholarPubMed
Strimmer, K. & Haeseler, A.V. (1996) Quartet puzzling: A quartet maximum likelihood method for reconstructing tree topologies. Molecular Biology and Evolution 13, 964–969.CrossRefGoogle Scholar
Swofford, D.L. (2002) PAUP*: Phylogenetic analysis using parsimony (and other methods. Sunderland, Massachusetts, Sinauer Associates.Google Scholar
Taffs, L.F. (1976) Pinworm infection in laboratory rodents: a review. Laboratory Animals 10, 1–13.CrossRefGoogle ScholarPubMed
Tatonova, Y.V., Chelomina, G.N. & Besprosvannykh, V.V. (2012) Genetic diversity of nuclear ITS1–5.8 S–ITS2 rDNA sequence in Clonorchis sinensis Cobbold, 1875 (Trematoda: Opisthorchidae) from the Russian Far East. Parasitology International 61, 664–674.CrossRefGoogle Scholar
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997) The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 24, 4876–4882.CrossRefGoogle Scholar
Wang, C.R., Lou, Y., Zhang, Y., Wang, W.T., Zheng, X., Xu, W.W., Zhang, Y., Tian, S.Q., Na, L. & Chang, Q.C. (2014) Genetic variability among Syphacia obvelata isolates from laboratory mice in four different geographical locations of China revealed by sequence analyses of five mitochondrial genes. Mitochondrial DNA [Epub ahead of print].Google ScholarPubMed
Zhao, G.H., Hu, B., Song, J.K., Jia, Y.Q., Li, H.M., Wang, C.R., Lin, Q., Xu, Q.X., Yu, S.K. & Deng, Y. (2014) Characterization of Oesophagostomum asperum and O. columbianum by internal transcribed spacers of nuclear ribosomal DNA. Journal of Helminthology 88, 74–81.CrossRefGoogle Scholar