Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-11-25T19:18:08.678Z Has data issue: false hasContentIssue false
  • English
  • Français

Restriction fragment length polymorphisms of Anisakinae larvae

Published online by Cambridge University Press:  05 June 2009

Kazuo Sugane ,
Liu Qing  and
Tadashi Matsuura
Kazuo Sugane
Affiliation:
Department of Parasitology, Faculty of Medicine, Shinshu University, Asahi 3–1–1, Matsumoto City, Nagano Prefecture, Japan
Liu Qing
Affiliation:
Department of Parasitology, Faculty of Medicine, Shinshu University, Asahi 3–1–1, Matsumoto City, Nagano Prefecture, Japan
Tadashi Matsuura
Affiliation:
Department of Parasitology, Faculty of Medicine, Shinshu University, Asahi 3–1–1, Matsumoto City, Nagano Prefecture, Japan
Get access Rights & Permissions [Opens in a new window]

Abstract

The analysis of restriction fragment length polymorphisms (RFLPs) was applied to distinguish several kinds of Anisakinae larvae, Anisakis larvae (type I) collected from two different paratenic hosts, Anisakis larvae (type II) and Contracaecum larvae. The patterns of the two different paratenic host-derived DNA of Anisakis larva (I) were exactly the same in hybridized fragments generated by six endonucleases. The quite different patterns in RFLPs of genomic DNA were observed among the Anisakis larva (I), Anisakis larva (II) and Contracaecum larvae. The results suggest that the RFLPs analysis may be useful for distinguishing Anisakinae larvae and clarifying the relationships between Anisakis larvae and their adult worms.

Keywords

AnisakislarvaeRFLPsContracaecumchemotaxonomy
Type
Research Article
Information
Journal of Helminthology , Volume 63 , Issue 4 , December 1989 , pp. 269 - 274
Copyright
Copyright © Cambridge University Press 1989

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Agatsuma, T. (1982) Electrophoretic studies on glucosephosphate isomerase and phosphoglucomutase in two types of Anisakis larvae. International Journal for Parasitology, 12, 35–39.Google ScholarPubMed
Cameron, M. L., Levy, P., Nutman, T., Vanamala, C. R., Narayanan, P. R. & Rajan, T. V. (1988) Use of restriction fragment length polymorphisms (RFLPs) to distinguish between nematodes of pathogenic significance. Parasitology, 96, 381–390.CrossRefGoogle ScholarPubMed
Davey, J. T. (1971) A revision of the genus Anisakis Dujardin, 1845 (Nematoda: Ascaridata). Journal of Helminthology, 45, 51–72.CrossRefGoogle Scholar
Files, J. C. & Hirsh, D. (1981) Ribosomal DNA of Caenorhabditis elegans. Journal of Molecular Biology, 149, 223–240.CrossRefGoogle ScholarPubMed
Gould, S. E. (1970) In: Trichinosis in man and animals, (editor, Gould, S. E.) pp. 190–221. Charles C. Thomas Publisher: Springfield, U.S.A.Google Scholar
Grummt, F. & Bielka, H. (1968) Some properties of ribosomes from eggs of Ascaris at different stages of development. Biochimica et Biophysica Acta, 161, 253–255.CrossRefGoogle ScholarPubMed
Kates, S., Wright, K. A. & Wright, R. (1973) A case of human infection with the cod nematode Phocanema sp. American Journal of Tropical Medicine and Hygiene, 22, 606–608.CrossRefGoogle ScholarPubMed
Koyama, T., Kobayashi, A., Kumada, M., Komiya, Y., Oshima, T., Kagei, N., Ishii, T. & Machida, M. (1969) Morphological and taxonomical studies on Anisakidae larvae found in marine fishes and squids. Japanese Journal of Parasitology, 18, 466–487.Google Scholar
Little, M. & Most, H. (1973) Anisakid larva from the throat of a woman in New York. American Journal of Tropical Medicine and Hygiene, 22, 609–612.CrossRefGoogle ScholarPubMed
Maniatis, T., Fritsch, E. F. & Sambrook, J. (1982) Molecular cloning. A laboratory manual. pp. 194–203. Cold Spring Harbor Laboratory, U.S.A.Google Scholar
Maxman, A. M. & Gilbert, W. (1980) Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology, 65, 499–506.Google Scholar
Mcdonnell, M. W., Simon, M. N. & Studier, F. W. (1977) Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. Journal of Molecular Biology, 110, 119–128.CrossRefGoogle Scholar
Orecchia, P., Paggi, L., Mattiucci, S., Smith, J. W., Nascetti, G. & Bullini, L. (1986) Electrophoretic identification of larvae and adults of Anisakis (Ascaridida: Anisakidae). Journal of Helminthology, 60, 331–339.CrossRefGoogle ScholarPubMed
Oshima, T. (1972) Anisakis and anisakiasis in Japan and adjacent area. In: Progress of Medical Parasitology in Japan (editors, Morishita, K., Komiya, Y. & Matsubayashi, H.), Vol. IV, pp. 301–393. Meguro Parasitological Museum, Tokyo.Google Scholar
Shiraki, T. (1974) Larval nematodes of family Anisakidae (Nematoda) in the northern sea of Japan as a causative agent of eosinophilic phlegmone or granuloma in the human gastro-intestinal tract. Acta Medica et Biologica, 22, 57–98.Google Scholar
Southern, E. M. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology, 98, 503–517.CrossRefGoogle ScholarPubMed
Suzuki, H., Ohnuma, H. & Karasawa, Y. (1972) Terranova (Nematoda: Anisakidae) infection in man. I. Clinical features of five cases of Terranova larva infection. Japanese Journal of Parasitology, 21, 252–256.Google Scholar
Van Thiel, P. H., Kuipers, F. C. & Roskam, T. H. (1960) A nematode parasitic to herring causing acute abdominal syndromes in man. Tropical and Geographical Medicine, 12, 97–113.Google ScholarPubMed
Van Thiel, P. H. (1962) Anisakiasis. Parasitology, 52, 16–17.Google Scholar