Hostname: page-component-cd9895bd7-lnqnp Total loading time: 0 Render date: 2024-12-25T02:07:34.193Z Has data issue: false hasContentIssue false
  • English
  • Français

Radiolabelling of the excretory-secretory and somatic antigens of Anisakis simplex larvae

Published online by Cambridge University Press:  05 June 2009

Kazuo Sugane ,
Shu-Han Sun  and
Tadashi Matsuura
Kazuo Sugane
Affiliation:
Department of Parasitology, Shinshu University School of Medicine, Asahi 3–1–1, Matsumoto City, Nagano Prefecture 390, Japan
Shu-Han Sun
Affiliation:
Department of Parasitology, Shinshu University School of Medicine, Asahi 3–1–1, Matsumoto City, Nagano Prefecture 390, Japan
Tadashi Matsuura
Affiliation:
Department of Parasitology, Shinshu University School of Medicine, Asahi 3–1–1, Matsumoto City, Nagano Prefecture 390, Japan
Get access Rights & Permissions [Opens in a new window]

Abstract

Anisakis simplex larvae were cultured in vitro in medium containing 35-methionine for ten days. The medium and the larval tissues were analysed for biosynthetically labelled polypeptide by sodium dodecyl sulphate polyacrylamide gel electrophoresis and autoradiography. Immunoprecipitates with positive and negative human antisera were similarly analysed, using Staphylococcus aureus to absorb immunocomplexes. ES products of Anisakis larvae contained many polypeptides with molecular weights of less than 200 K. 180 KDa and 40 KDa polypeptides in ES products reacted with IgG in Anisakis-infected human sera. Somatic extracts also contained many polypeptides with molecular weights of less than 200 K. One of these polypeptides with a molecular weight of 130 K reacted with IgG in Anisakis-infected human sera. These polypeptides did not react with other nematode-infected human sera.

Keywords

Anisakis simplexlarvaeES antigensomatic antigenradiolabellingNematoda
Type
Research Article
Information
Journal of Helminthology , Volume 66 , Issue 4 , December 1992 , pp. 305 - 309
Copyright
Copyright © Cambridge University Press 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Akao, N., Ohyama, T. & Kondo, K. (1990) Immunoblot analysis of serum IgG. IgA and IgE responses against larval excretory-secretory antigens of Anisakis simplex in patients with gastric anisakiasis. Journal of Helminthology, 64, 310–318.CrossRefGoogle ScholarPubMed
Irving, D. O. & Howell, M. J. (1982) Characterization of excretory-secretory antigens of Fasciola hepatica. Parasitology. 85, 179–188.CrossRefGoogle ScholarPubMed
Kennedy, M. W.Tierney, J., Ye, P., Mcnonagle, F. A.Mcclaughlin, D. & Smith, J. W. (1988) The secreted and somatic antigens of the third stage larva of Anisakis simplex, and antigenic relationship with Ascaris suum, Ascaris lumbricoides, and Toxocara canis. Molecular and Biochemical Parasitology, 31, 35–46.CrossRefGoogle ScholarPubMed
Kessler, S. W. (1975) Rapid isolation of antigens from cells with a staphylococcal protein-A-antibody absorbent: Parameters of the interaction of antibody-antigen complexes with protein A. Journal of Immunology, 115, 1617–1624.Google Scholar
Lowry, O. H., Rosenbrough, N. J., Farr, A. L. & Randall, R. J. (1951) Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 193, 265–275.CrossRefGoogle ScholarPubMed
Oshima, T. (1972) Anisakis and anisakiasis in Japan and adjacent area. In: Progress of Medical Parasitology. (Editors, Morishita, K., Koyama, Y. & Matsubayashi, H) vol. 4, pp. 301–393. Meguro Parasitological Museum; Tokyo.Google Scholar
Oshima, T. (1987) Anisakiasis—Is the sushi bar guilty? Parasitology Today, 3, 44–48.Google Scholar
Sakanari, J. A. (1990) Anisakis—from the platter to the microfuge. Parasitology Today, 6, 323–327.Google Scholar
Sugane, K., Howell, M. J. & Nicholas, W. L. (1985) Biosynthetic labelling of the excretory and secretory antigens of Toxocara canis larvae. Journal of Helminthology, 59, 147–151.CrossRefGoogle ScholarPubMed
Takahashi, S., Sato, N. & Ishikura, H. (1986) Establishment of monoclonal antibodies that discriminate the antigen distribution specifically found in Anisakis larvae (type 1). Journal of Parasitology, 72, 960–962.CrossRefGoogle Scholar
Takahashi, S., Ishikura, H. & Hayasaka, H. (1989) Monoclonal antibody, intradermal reaction, and Sarles' phenomenon. In: Gastric Anisakiasis in Japan. (Editors, Ishikura, H. & Namiki, M.) pp. 103–111. Springer-Verlag: Tokyo.CrossRefGoogle Scholar
Yagihashi, A., Sato, N., Takahashi, S., Ishikura, H. & Kikuchi, K. (1990) A serodiagnostic assay by microenzyme-linked immunosorbent assay for human anisakiasis using a monoclonal antibody specific for Anisakis larvae antigen. Journal of Infectious Diseases, 161, 995–998.Google Scholar