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Proteolytic activity in Fasciola hepatica is reduced by the administration of cathepsin L mimotopes

Published online by Cambridge University Press:  26 April 2010

A. Villa-Mancera ,
H. Quiroz-Romero ,
D. Correa  and
R.A. Alonso
A. Villa-Mancera*
Affiliation:
Laboratorio de Genética y Reproducción, Facultad de Medicina Veterinaria y Zootecnia, Benemérita Universidad Autónoma de Puebla, 4 Sur 304 Col. Centro, C.P. 75482, Tecamachalco Puebla, México Departamento de Parasitología, Facultad de Medicina Veterinaria y Zootecnia, UNAM, Av. Universidad 3000, Col. Copilco Universidad, C.P. 04510, México D.F.
H. Quiroz-Romero
Affiliation:
Departamento de Parasitología, Facultad de Medicina Veterinaria y Zootecnia, UNAM, Av. Universidad 3000, Col. Copilco Universidad, C.P. 04510, México D.F.
D. Correa
Affiliation:
Laboratorio de Inmunología Experimental, Instituto Nacional de Pediatría, Av. Insurgentes Sur 3700-C, Col. Insurgentes Cuicuilco, C.P. 04530, México D.F.
R.A. Alonso
Affiliation:
Departamento de Genética y Bioestadística, Facultad de Medicina Veterinaria y Zootecnia, UNAM, Av. Universidad 3000, Col. Copilco Universidad, C.P. 04510, México D.F.
*
*Fax: +52 (249) 422-0178 E-mail: [email protected]
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Abstract

The objective of this study was to assess the proteolytic activity of Fasciola hepatica cathepsins in liver sections from mice vaccinated with phage clones of cathepsin L mimotopes, using the film in situ zymography technique. Female BALB/c mice were immunized three times with 2.5 × 1011 phage particles without adjuvant. Animals vaccinated with phage clones produced high titres of anti-mimotope antibodies and a significant reduction in fluke burden was observed following challenge with metacercariae of F. hepatica. The proteolytic activity in hepatic tissue was reduced after the immunization with phage clones.

Type
Research Papers
Information
Journal of Helminthology , Volume 85 , Issue 1 , March 2011 , pp. 51 - 55
Copyright
Copyright © Cambridge University Press 2010

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