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Proteolytic activity in Fasciola hepatica is reduced by the administration of cathepsin L mimotopes

Published online by Cambridge University Press:  26 April 2010

A. Villa-Mancera ,
H. Quiroz-Romero ,
D. Correa  and
R.A. Alonso
A. Villa-Mancera*
Affiliation:
Laboratorio de Genética y Reproducción, Facultad de Medicina Veterinaria y Zootecnia, Benemérita Universidad Autónoma de Puebla, 4 Sur 304 Col. Centro, C.P. 75482, Tecamachalco Puebla, México Departamento de Parasitología, Facultad de Medicina Veterinaria y Zootecnia, UNAM, Av. Universidad 3000, Col. Copilco Universidad, C.P. 04510, México D.F.
H. Quiroz-Romero
Affiliation:
Departamento de Parasitología, Facultad de Medicina Veterinaria y Zootecnia, UNAM, Av. Universidad 3000, Col. Copilco Universidad, C.P. 04510, México D.F.
D. Correa
Affiliation:
Laboratorio de Inmunología Experimental, Instituto Nacional de Pediatría, Av. Insurgentes Sur 3700-C, Col. Insurgentes Cuicuilco, C.P. 04530, México D.F.
R.A. Alonso
Affiliation:
Departamento de Genética y Bioestadística, Facultad de Medicina Veterinaria y Zootecnia, UNAM, Av. Universidad 3000, Col. Copilco Universidad, C.P. 04510, México D.F.
*
*Fax: +52 (249) 422-0178 E-mail: [email protected]
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Abstract

The objective of this study was to assess the proteolytic activity of Fasciola hepatica cathepsins in liver sections from mice vaccinated with phage clones of cathepsin L mimotopes, using the film in situ zymography technique. Female BALB/c mice were immunized three times with 2.5 × 1011 phage particles without adjuvant. Animals vaccinated with phage clones produced high titres of anti-mimotope antibodies and a significant reduction in fluke burden was observed following challenge with metacercariae of F. hepatica. The proteolytic activity in hepatic tissue was reduced after the immunization with phage clones.

Type
Research Papers
Information
Journal of Helminthology , Volume 85 , Issue 1 , March 2011 , pp. 51 - 55
Copyright
Copyright © Cambridge University Press 2010

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References

Alcalá-Canto, Y., Ibarra-Velarde, F., Gracia-Mora, J. & Sumano-López, H. (2005) Fasciola hepatica proteolytic activity in liver revealed by in situ zymography. Parasitology Research 96, 308–311.CrossRefGoogle ScholarPubMed
Alcalá-Canto, Y., Ibarra-Velarde, F., Sumano-Lopez, H., Gracia-Mora, J. & Alberti-Navarro, A. (2006) Dose-response inhibition of proteolytic activity by a cysteine protease inhibitor in a murine model of fasciolosis. Parasitology Research 98, 438–442.CrossRefGoogle Scholar
Berasaín, P., Goni, F., McGonigle, S., Dowd, A., Dalton, J.P., Frangione, B. & Carmona, C. (1997) Proteinases secreted by Fasciola hepatica degrade extracellular matrix and basement membrane components. Journal of Parasitology 83, 1–5.CrossRefGoogle ScholarPubMed
Carmona, C., Dowd, A.J., Smith, A.M. & Dalton, J.P. (1993) Cathespin L proteinase secreted by Fasciola hepatica in vitro prevents antibody mediated eosinophil attachment to newly excysted juveniles. Molecular and Biochemical Parasitology 62, 9–18.CrossRefGoogle Scholar
Cheung, A.L., Ying, P. & Fischetti, V.A. (1991) A method to detect proteinase activity using unprocessed X-ray films. Analytical Biochemistry 193, 20–23.CrossRefGoogle ScholarPubMed
Dalton, J.P. & Heffernan, M. (1989) Thiol proteases released in vitro by Fasciola hepatica. Molecular and Biochemical Parasitology 35, 161–166.CrossRefGoogle ScholarPubMed
Dalton, J.P., McGonigle, S., Rolph, T.P. & Andrews, S.J. (1996) Induction of protective immunity in cattle against infection with Fasciola hepatica by vaccination with cathepsin L proteinases and with haemoglobin. Infection and Immunity 64, 5066–5074.CrossRefGoogle Scholar
Dalton, J.P., O'Neill, S., Stack, C., Collins, P., Walshe, A., Sekiya, M., Doyle, S., Mulcahy, G., Hoyle, D., Khaznadji, E., Moiré, N., Brennan, G., Mousley, A., Kreshchenko, N., Maule, A.G. & Donnelly, S.M. (2003) Fasciola hepatica cathepsin L-proteases: biology, function, and potential in the development of first generation liver fluke vaccines. International Journal for Parasitology 33, 1173–1181.CrossRefGoogle ScholarPubMed
De Groot, A.S. (2004) Immunome-derived vaccines. Expert Opinion on Biological Therapy 4, 767–772.CrossRefGoogle ScholarPubMed
Devlin, J.J., Panganiban, L.C. & Delvin, P.E. (1990) Random peptide libraries: a source of specific protein binding molecules. Science 249, 404–406.CrossRefGoogle ScholarPubMed
Dowd, A.J., Smith, A.M., McGonigle, S. & Dalton, J.P. (1994) Purification and characterization of a second cathepsin L proteinase secreted by the parasitic trematode Fasciola hepatica. European Journal of Biochemistry 223, 91–98.CrossRefGoogle ScholarPubMed
George, S.J. & Johnson, J.L. (2001) In situ zymography. Methods in Molecular Biology 151, 411–415.Google ScholarPubMed
Hillyer, G.V. (2005) Fasciola antigens as vaccines against fascioliasis and schistosomiasis. Journal of Helminthology 79, 241–247.CrossRefGoogle ScholarPubMed
Hoyle, D.V., Dalton, J.P., Chase-Topping, M. & Taylor, D.W. (2002) Preexposure of cattle to drug-abbreviated Fasciola hepatica infections: the effect upon subsequent challenge infection and early immune response. Veterinary Parasitology 111, 65–82.CrossRefGoogle Scholar
McManus, D.P. & Dalton, J.P. (2006) Vaccines against the zoonotic trematodes Schistosoma japonicum, Fasciola hepatica and Fasciola gigantica. Parasitology 133, S43–S61.CrossRefGoogle ScholarPubMed
Mulcahy, G., O'Connor, F., McGonigle, S., Dowd, A.J., Clery, D., Andrews, S.J. & Dalton, J.P. (1998) Correlation of specific antibody titre and avidity with protection in cattle immunized against Fasciola hepatica. Vaccine 16, 932–939.CrossRefGoogle ScholarPubMed
Mulcahy, G., Joyce, P. & Dalton, J.P. (1999) Immunology of Fasciola hepatica infection. pp. 341–376in Dalton, J.P. (Ed.) Fasciolosis. Wallingford, CABI Publishing.Google Scholar
O'Neill, S.M., Brady, M.T., Callanan, J.J., Mills, K.H.G. & Dalton, J.P. (2000) Fasciola hepatica infection down-regulates Th1 response in mice. Parasite Immunology 22, 147–155.CrossRefGoogle Scholar
Pucci-Minafra, I., Minafra, S., La Rocca, G., Barranca, M., Fontana, S., Alaimo, G. & Okada, Y. (2001) Zymographic analysis of circulating and tissue forms of colon carcinoma gelatinase A (MMP-2) and B (MMP-9) separated by mono and two-dimensional electrophoresis. Matrix Biology 20, 419–427.CrossRefGoogle Scholar
Sidhu, S.S. & Koide, S. (2007) Phage display for engineering and analyzing protein interaction interfaces. Current Opinion in Structural Biology 17, 481–487.CrossRefGoogle ScholarPubMed
Smith, A.M., Dowd, A.J., Heffernan, M., Robertson, C.D. & Dalton, J.P. (1993) Fasciola hepatica: a secreted cathepsin L-like proteinase cleaves host immunoglobulin. International Journal for Parasitology 23, 977–983.CrossRefGoogle ScholarPubMed
Smith, G.P. & Pretenko, V.A. (1997) Phage display. Chemical Reviews 97, 391–410.CrossRefGoogle ScholarPubMed
Torgerson, P.R. & Claxton, J.R. (1999) Epidemiology and control. pp. 113–149in Dalton, J.P. (Ed.) Fasciolosis. Wallingford, CABI Publishing.Google Scholar
Villa-Mancera, A., Quiroz-Romero, H., Correa, D., Ibarra, F., Reyes-Pérez, H., Reyes-Vivas, H., López-Velázquez, G., Gazarian, K., Gazarian, T. & Alonso, R.A. (2008) Induction of immunity in sheep to Fasciola hepatica with mimotopes of cathepsin L selected from a phage display library. Parasitology 135, 1437–1445.CrossRefGoogle ScholarPubMed
Wijffels, G.L., Salvatore, L., Dosen, M., Waddington, J., Wilson, L., Thompson, C., Campbell, N., Sexton, J., Wicker, J., Bowen, F., Friedel, T. & Spithill, T.W. (1994) Vaccination of sheep with purified cysteine proteinases of Fasciola hepatica decreases worm fecundity. Experimental Parasitology 78, 132–148.CrossRefGoogle ScholarPubMed
Yamasaki, H., Aoki, T. & Oya, H. (1989) A cysteine proteinase from the liver fluke Fasciola spp.: purification, characterization, localization and application to immunodiagnosis. Japanese Journal of Parasitology 38, 373–384.Google Scholar
Yan, S.J. & Blomme, E.A.G. (2003) In situ zymography: A molecular pathology technique to localize endogenous protease activity in tissue sections. Veterinary Pathology 40, 227–236.CrossRefGoogle ScholarPubMed