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The protective capacity of UV-exposed Clinostomum complanatum metacercariae against challenge infections in Ardeola ibis ibis

Published online by Cambridge University Press:  13 May 2009

G. Allam  and
S.M. Aboel Hadid
G. Allam*
Affiliation:
College of Medicine and Medical Sciences, Taif University, Taif, Saudi Arabia Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt
S.M. Aboel Hadid
Affiliation:
Parasitology Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt
*
*Fax: 00966-27250528 E-mail: [email protected]
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Abstract

Exposure of encysted metacercariae of Clinostomum complanatum to UV light (254 nm) for 60 min reduced their development into adult worms in buff-backed herons (95.7% reduction in worm burden). Metacercariae that succeeded in developing into adult worms, showed low fecundity levels. Furthermore, 30% of eggs laid showed abnormal shape; however, all normal and abnormal eggs failed to hatch into miracidia. The effectiveness of UV-irradiated metacercariae as a vaccine was investigated. Compared to control unvaccinated herons, the vaccinated group showed a significantly high protection rate (73.8%) against challenge. In vitro, worm development after challenge showed decreased fecundity and increased egg abnormalities, where only 1.5% of all eggs produced hatched into miracidia. A passive haemagglutination test revealed increased antibody titres against soluble adult worm antigen in both vaccinated and vaccinated-challenged birds. It was concluded that vaccination of herons using encysted metacercariae UV-irradiated for 60 min can protect them against challenge infection.

Type
Research Papers
Information
Journal of Helminthology , Volume 83 , Issue 4 , December 2009 , pp. 361 - 367
Copyright
Copyright © Cambridge University Press 2009

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References

Abd El-Galil, M.A., Kamel, H.H., Abd El-Rahman, A.H. & Fouda, M. (2007) A trial for treatment of yellow grub disease in Oreochromis niloticus by praziquantel. Egyptian Journal of Aquatic Biology and Fish 11, 801–815.Google Scholar
Aboel Hadid, S.M. & Lotfy, H.S. (2007) Some studies helminth parasites of buff backed heron (Ardeola ibis ibis) with special reference to its role in transmission of Clinostomum complanatum in Beni-Suef Governorate. Beni-Suef Veterinary Medicine Journal 5, 135–141.Google Scholar
A/Gadir, H., Haroun, E.M. & Gameel, A.A. (1987) The protective effect of irradiated metacercariae of Fasciola gigantica against homologous challenge in sheep. Journal of Helminthology 61, 137–142.CrossRefGoogle ScholarPubMed
Amato, J.F., Boeger, W.A. & Amato, S.B. (1991) Protocolos para laboratório-coletae processamento de parasitas do pescado. 81 p. Rio de Janeiro, Imprensa Universitária, Universidade Federal do Rio de Janeiro.Google Scholar
Aohagi, Y., Shibahara, T. & Machida, N. (1992) Natural infections of Clinostomum complanatum (Trematoda: Clinostomatidae) in wild herons and egrets, Tottori Prefecture, Japan. Journal of Wildlife Diseases 28, 470–471.CrossRefGoogle ScholarPubMed
Auerbach, C. (1976) Mutation research: problems, results and perspective. pp. 170–244. 1st edn.London, Chapman and Hall.CrossRefGoogle Scholar
Barber, I., Hoare, D. & Krause, J. (2000) Effects of parasites on fish behavior: a review and evolutionary perspective. Journal of Fish Biology 10, 131–165.CrossRefGoogle Scholar
Boyden, S.V. (1951) The adsorption of proteins on erythrocytes treated with tannic acid and subsequent haemagglutination by antiprotein sera. Journal of Experimental Medicine 93, 107.CrossRefGoogle ScholarPubMed
Bradford, M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principles of protein-dye binding. Analytical Biochemistry 72, 248–254.CrossRefGoogle ScholarPubMed
Chung, D.I., Kong, H.H. & Moon, C.H. (1995) Demonstration of the second intermediate hosts of Clinostomum complanatum in Korea. Korean Journal of Parasitology 33, 305–312.CrossRefGoogle ScholarPubMed
Coulson, P.S. (1997) The radiation-attenuated vaccine against schistosomes in animal models: paradigm for a human vaccine? Advances in Parasitology 39, 271–336.CrossRefGoogle ScholarPubMed
Creaney, J., Spithill, W.T., Thompson, M.C., Wilson, R.L., Sandeman, M. & Parsons, C.J. (1995) Attempted immunisation of sheep against Fasciola hepatica using γ-irradiated metacercariae. International Journal for Parasitology 25, 853–856.CrossRefGoogle ScholarPubMed
Dean, D.A. (1983) A review: schistosomiasis and related genera: acquired resistance in mice. Experimental Parasitology 55, 1–104.CrossRefGoogle ScholarPubMed
Dean, D.A., Murrell, K.D. & Xu, S.T. (1983) Immunization of mice with ultraviolet-irradiated Schistosoma mansoni cercariae: a re-evaluation. American Journal of Tropical Medicine and Hygiene 32, 790–793.CrossRefGoogle ScholarPubMed
Dean, D.A., Mangold, B.L., Harrison, R.A. & Ricciardone, M.D. (1996) Homologous and heterologous protective immunity to Egyptian strains of Schistosoma mansoni and S. haematobium induced by ultraviolet-irradiated cercariae. Parasite Immunology 18, 403–410.CrossRefGoogle ScholarPubMed
Diffey, B.L (1991) Solar ultraviolet radiation effects on biological systems. Physics in Medicine and Biology 36, 299–328.CrossRefGoogle ScholarPubMed
Eiras, J.C. (1994) Elementos de ictioparasitologia. 339 p. Porto, Portugal, Fundação Eng. António de Almeida.Google Scholar
el Ridi, R., Ozaki, T., Sato, H., Inaba, T. & Kamiya, H. (1997) Immunization of mice with ultraviolet-attenuated cercariae of Schistosoma mansoni transiently reduces the fecundity of challenge worms. International Journal for Parasitology 27, 581–586.CrossRefGoogle ScholarPubMed
Errera, M. (1958) Etude photochemique de l'acide desoxyribonucleique: I. Mesures energetiques. Biochimica et Biophysica Acta 8, 30–37.CrossRefGoogle Scholar
Freifelder, D. (1987) Molecular biology. pp. 277–292. 2nd edn.Boston, Jones and Bartlett.Google Scholar
Harm, W. (1980) Biological effects of ultraviolet radiation. Cambridge, Cambridge University Press.Google Scholar
Hendrix, C.M. (1998) Diagnostic veterinary parasitology. pp. 225–2542nd edn. St. Louis, Missouri, USA, Charles Mosby Inc.Google Scholar
Jankovic, D., Wynn, T.A., Kullberg, M.C., Hieny, S., Caspar, P., James, S., Cheever, A.W. & Sher, A. (1999) Optimal vaccination against Schistosoma mansoni requires the induction of both B cells and IFN-gamma-dependent effector mechanisms. Journal of Immunology 162, 345–351.CrossRefGoogle Scholar
Jian, X., Jun-Min, Y., Hai-Chou, X., Hui-Qing, Q., Hai-Nan, R., Hotez, P., Bin, Z. & Shu-Hua, X. (2006) Protective immunity elicited by ultraviolet-irradiated third-stage infective hookworm (Necator americanus and Ancylostoma caninum) larvae in mice and hamsters. Southeast Asian Journal of Tropical Medicine and Public Health 37, 885–895.Google Scholar
Kamiya, H., Ozaki, T., Nakayama, H. & Inaba, T. (1993) Immunizing potential of ultraviolet-attenuated cercariae of Schistosoma mansoni in rodent hosts. Parasitology Research 79, 293–296.CrossRefGoogle ScholarPubMed
Kitagawa, N., Oda, M., Totoki, T., Washizaki, S., Oda, M. & Kifune, T. (2003) Lidocaine spray used to capture a live Clinostomum parasite causing human laryngitis. American Journal of Otolaryngology 24, 341–343.CrossRefGoogle ScholarPubMed
Konno, K., Oku, Y., Sakai, H. & Kamiya, M. (1997) Effect of ultraviolet radiation on the infectivity of Taenia taeniaeformis eggs. Japanese Journal of Veterinary Research 45, 75–79.Google ScholarPubMed
Kumagai, M., Sato, H. & Kamiya, H. (1992) Migration kinetics of ultraviolet-attenuated Schistosoma mansoni in ICR mice. Japanese Journal of Parasitology 41, 300–305.Google Scholar
Lagapa, J.T., Konno, K., Oku, Y., Nonaka, N. & Kamiya, M. (2001) Inhibitory effect of different UV lamps on the infectivity of taeniid eggs. Parasitology Research 87, 593–597.CrossRefGoogle ScholarPubMed
Lehmann, A.R. & Norris, P.G. (1990) DNA repair deficient photodermatoses. Seminars in Dermatology 9, 55–62.Google ScholarPubMed
McLaren, D.J. & Smithers, S.R. (1988) Serum from CBA/Cd mice vaccinated with irradiated cercariae of Schistosoma mansoni protects naive recipients through the recruitment of cutaneous effector cells. Parasitology 97, 287–302.Google Scholar
Menon, S. & Bhopale, M.K. (1985) Efficacy of UV-irradiated larval vaccine of Ancylostoma ceylanicum (Looss, 1911) in golden hamsters (Mesocricetus auratus). Journal of Helminthology 59, 287–293.CrossRefGoogle ScholarPubMed
Nabih, I. & Abd El-Hamid, A. (1984) Biochemical studies on the genetic material of fresh water snails intermediate hosts of schistosomiasis. I. Effect of UV-radiation as physical mutagen on the chemical nature of DNA. Cellular and Molecular Biology 30, 33–36.Google Scholar
Nakayama, H., Inaba, T., Nargis, M., Chisty, M., Ito, M. & Kamiya, H. (1998) Immunization of laboratory animals with ultraviolet-attenuated larvae against homologous challenge infection with Trichinella britovi. Southeast Asian Journal of Tropical Medicine and Public Health 29, 563–566.Google ScholarPubMed
Nilsson, A. (1996) Ultraviolet reflections: life under a thinning ozone layer. pp. 26–37. 1st edn.NewYork, USA, Wiley and Sons.Google Scholar
Patrick, M.H. & Rahn, R.O. (1976) Photochemistry of DNA and polynucleotides photoproducts. pp. 35–95in Wang, S.Y. (Ed.) Photochemistry and photobiology of nucleic acids. Vol. 2, New York, Academic Press.CrossRefGoogle Scholar
Richter, D., Incani, R.N. & Harn, D.A. (1993) Isotype responses to candidate vaccine antigens in protective sera obtained from mice vaccinated with irradiated cercariae of Schistosoma mansoni. Infection and Immunity 61, 3003–3011.CrossRefGoogle ScholarPubMed
Richter, D., Harn, D. & Matuschka, F. (1995) The irradiated cercariae vaccine model: looking on the bright side of radiation. Parasitology Today 11, 288–290.CrossRefGoogle ScholarPubMed
Salih, S.Y., Bartlett, A. & Voller, A. (1978) Detection of antibodies by enzyme immunoassay in human S. mansoni infection: a clinical and chemo-therapeutic study. Tropenmedizin und Parasitologie 29, 409–412.Google Scholar
Sanderson, L., Bartlett, A. & Whitfield, P.J. (2002) In vitro and in vivo studies on the bioactivity of a ginger (Zingiber officinale) extract towards adult schistosomes and their egg production. Journal of Helminthology 76, 241–247.CrossRefGoogle ScholarPubMed
Sher, A., Hieny, S., Jame, S.L. & Asofsky, R. (1982) Mechanism of protective immunity against Schistosomiasis mansoni infection in mice vaccinated with irradiated cercariae. II. Analysis of immunity in hosts deficient in T lymphocytes, B-lymphocytes, or complement. Journal of Immunology 128, 1800–1884.Google ScholarPubMed
Shi, Y.E., Jiang, C.F., Han, J.J., Li, Y.L. & Ruppel, A. (1990) Schistosoma japonicum: an ultraviolet-attenuated cercarial vaccine applicable in the field for water buffaloes. Experimental Parasitology 71, 100–106.CrossRefGoogle ScholarPubMed
Shirai, R., Matsubara, K., Ohnishi, T., Nishiyama, H., Miyamoto, J., Watanabe, A., Harada, R., Kadota, J. & Kohno, S. (1998) A case of human infection with Clinostomum sp. Kansenshogaku Zasshi 72, 1242–1245.CrossRefGoogle ScholarPubMed
Silva-Souza, A.T. & Ludwig, G. (2005) Parasitism of Cichlasoma paranaense Kullander, 1983 and Gymnotus carapo Linnaeus, 1814 by Clinostomum complanatum (Rudolphi, 1814) metacercariae in the Taquari river. Brazilian Journal of Biology 65, 513–519.CrossRefGoogle ScholarPubMed
Smith, K.C. (1966) Physical chemical changes induced in nucleic acids by ultraviolet light. Radiation Research 6, 54–79.Google Scholar
Soulsby, E.J.L. (1982) Helminthes, arthropodes and protozoa of domestic animals. 773 p. 7th edn.London, The English Language Book Society and Baillères Tindall.Google Scholar
Takatsy, G. (1956) The use of spiral loops in serological and virological micromethods. Acta Microbiologica Hungarica 3, 191.Google Scholar
Yamaguti, S. (1971) Synopsis of digenetic trematodes of vertebrates. 1072 p. Tokyo Japan, Keigakur Publishing Company.Google Scholar
Younis, S.A., Yagi, A.I., Haroun, E.M., Gameel, A.A. & Taylor, M.G. (1986) Immunization of zebu calves against Fasciola gigantica, using irradiated metacercariae. Journal of Helminthology 60, 123–134.CrossRefGoogle ScholarPubMed