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Prevalence and molecular characterization of Spirometra erinaceieuropaei spargana in snakes in Hunan Province, China

Published online by Cambridge University Press:  27 February 2020

W. Liu ,
L. Tan ,
Y. Huang ,
W.C. Li ,
Y.S. Liu  and
L.C. Yang Open the ORCID record for L.C. Yang [Opens in a new window]
W. Liu
Affiliation:
Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan410128, China Hunan Co-Innovation Center of Animal Production Safety, Changsha, China College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
L. Tan
Affiliation:
Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan410128, China Hunan Co-Innovation Center of Animal Production Safety, Changsha, China
Y. Huang
Affiliation:
College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
W.C. Li
Affiliation:
Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan410128, China
Y.S. Liu
Affiliation:
Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan410128, China Hunan Co-Innovation Center of Animal Production Safety, Changsha, China
L.C. Yang*
Affiliation:
Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan410128, China Hunan Co-Innovation Center of Animal Production Safety, Changsha, China
*
Author for correspondence: L. Yang, E-mail: [email protected]
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Abstract

Sparganosis is an important foodborne parasitic zoonosis; however, few reports on the prevalence of snake-infecting plerocercoids from Hunan province in China are available. Therefore, we investigated the prevalence of spargana infection in wild snakes from this region in 2018, and identified an astonishing prevalence rate of 91.7% (344/375). Spargana parasites were found in 99.1% of Zaocys dhumnades, 94.1% of Elaphe carinata and 86.7% of Elaphe taeniura. Parasites exhibited various distributions: 50% were located in muscular tissue, 32.1% in subcutaneous tissue and 17.9% in the coelomic cavity. To identify the specific status of spargana collected from wild snakes, partial mitochondrial cytochrome c oxidase subunit 1 (cox1) gene sequences were amplified, sequenced and analysed. Sequence variations for cox1 among all the examined plerocercoids ranged between 0.0 and 2.9%, with 21 variable sites identified (4.71%, 21/446). Phylogenetic analyses identified that all plerocercoids isolated from Hunan province were Spirometra erinaceieuropaei. This is the first report of S. erinaceieuropaei infection in snakes in Hunan province. The risks and harms of sparganosis should be publicized, and illegal wildlife trade should be controlled.

Keywords

Hunan provincepleroceroidSpirometra einaceieuropaeimitochondrial genephylogenetic analysis
Type
Short Communication
Information
Journal of Helminthology , Volume 94 , 2020 , e131
Copyright
Copyright © The Author(s) 2020. Published by Cambridge University Press

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References

Bowles, J, Blair, D and McManus, DP (1992) Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Molecular and Biochemical Parasitology 54, 165–173.CrossRefGoogle ScholarPubMed
Daly, JJ (1982) Sparganosis. Vol l. Boca Raton, CRC Press.Google Scholar
Jeon, HK, Park, H, Lee, D, et al. (2018) Genetic and morphologic identification of Spirometra ranarum in Myanmar. The Korean Journal of Parasitology 56, 275–280.CrossRefGoogle ScholarPubMed
Kim, JG, Ahn, CS, Sohn, WM, Nawa, Y and Kong, Y (2018) Human sparganosis in Korea. Journal of Korean Medical Science 33, e273.CrossRefGoogle ScholarPubMed
Kołodziej-Sobocińska, M, Stojak, J, Kondzior, E, Ruczyńska, I and Wójcik, J (2019) Genetic diversity of two mitochondrial DNA genes in Spirometra erinaceieuropaei (Cestoda: Diphyllobothridae) from Poland. Journal of Zoological Systematics and Evolutionary Research 57, 764–777.CrossRefGoogle Scholar
Li, L, Yu, LY, Zhu, XQ, Wang, CR, Zhai, YQ and Zhao, JP (2008) Orientobilharzia turkestanicum is grouped within African schistosomes based on phylogenetic analyses using sequences of mitochondrial genes. Parasitology Research 102, 939–943.CrossRefGoogle ScholarPubMed
Li, MW, Song, HQ, Li, C, Lin, HY, Xie, WT, Lin, RQ and Zhu, XQ (2011) Sparganosis in mainland China. International Journal of Infectious Diseases 15, e154–e156.CrossRefGoogle ScholarPubMed
Liu, W, Zhao, GH, Tan, MY, Zeng, DL, Wang, KZ, Yuan, ZG, Lin, RQ, Zhu, XQ and Liu, Y (2010) Survey of Spirometra erinaceieuropaei spargana infection in the frog Rana nigromaculata of the Hunan Province of China. Veterinary Parasitology 173, 152–156.CrossRefGoogle ScholarPubMed
Liu, Q, Li, MW, Wang, ZD, Zhao, GH and Zhu, XQ (2015) Human sparganosis, a neglected food borne zoonosis. Lancet Infectious Diseases 15, 1226–1235.CrossRefGoogle ScholarPubMed
Lu, G, Shi, DZ, Lu, YJ, Wu, LX, Li, LH, Rao, LY and Yin, FF (2014) Retrospective epidemiological analysis of sparganosis in mainland China from 1959 to 2012. Epidemiology and Infection 142, 2654–2714.CrossRefGoogle ScholarPubMed
Mo, ZS, Li, XH, Lei, ZY and Xie, DY (2013) Clinical analysis of 25 sparganosis cases. Chinese Journal of Parasitology & Parasitic Diseases 31, 218–220.Google ScholarPubMed
Page, RD (1996) TreeView: an application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12, 357–358.Google ScholarPubMed
Su, HL and Li, CQ (2004) Research on pharmacological action and application of snake gall. Northwest Pharmacological Journal 19, 285–287.Google Scholar
Tan, ZR, Chen, CG and Ding, HF (2015) A Case of visceral sparganosis misdiagnosed as tuberculous pericarditis. Journal of Critical Care in Internal Medicine 21, 154–158.Google Scholar
Thompson, JD, Gibson, TJ, Plewniak, F, Jeanmougin, F and Higgins, DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25, 4876–4882.CrossRefGoogle ScholarPubMed
Wang, F, Zhou, L, Gong, S, Deng, Y, Zou, J, Wu, J, Liu, W and Hou, F (2011) Severe infection of wild-caught snakes with Spirometra erinaceieuropaei from food markets in Guangzhou, China involves a risk for zoonotic sparganosis. Journal of Parasitology 97, 170–171.CrossRefGoogle ScholarPubMed
Wang, F, Li, W, Hua, L, Gong, S, Xiao, J, Hou, F, Ge, Y and Yang, G (2014) Spirometra (Pseudophyllidea, Diphyllobothriidae) severely infecting wild-caught snakes from food markets in Guangzhou and Shenzhen, Guangdong, China: implications for public health. Scientific World Journal 2014, 874014.Google ScholarPubMed
Wicht, B, Ruggeri-Bernardi, N, Yanagida, T, Nakao, M, Peduzzi, R and Ito, A (2010) Inter- and intra-specific characterization of tapeworms of the genus Diphyllobothrium (Cestoda: Diphyllobothriidea) from Switzerland, using nuclear and mitochondrial DNA targets. Parasitology International 59, 35–39.CrossRefGoogle ScholarPubMed
Wu, ZJ, Chen, Y, Qiu, XL and Jiang, HT (2007) An investigation of plerocercoid infection of frogs in Guiyang city and an analysis on clinical characteristics of 104 cases. Journal of Guiyang Medical College 32, 140–141.Google Scholar
Yamasaki, H and Kuramochi, T (2009) A case of Diphyllobothrium nihonkaiense infection possibly linked to salmon consumption in New Zealand. Parasitology Research 105, 583–586.CrossRefGoogle ScholarPubMed
Yang, GD, Wang, FM, Gong, SP, Li, WY, Wei, YF, Ge, Y and Xiao, JJ (2015) Investigation of Spirometra mansoni infection in snakes from Guangdong Province. Guangdong Forestry Science and Technology 31, 80–83.Google Scholar
Zhang, X, Wang, H, Cui, J, Jiang, P, Fu, GM, Zhong, K, Zhang, ZF and Wang, ZQ (2015) Characterisation of the relationship between Spirometra erinaceieuropaei and Diphyllobothrium species using complete cytb and cox1 genes. Infection, Genetics and Evolution 35, 1–8.CrossRefGoogle ScholarPubMed
Zhu, XQ, Beveridge, I, Berger, L, Barton, D and Gasser, RB (2002) Single-strand conformation polymorphism-based analysis reveals genetic variation within Spirometra erinacei (Cestoda: Pseudophyllidea) from Australia. Molecular and Cellular Probes 16, 159–165.CrossRefGoogle ScholarPubMed