Hostname: page-component-78c5997874-j824f Total loading time: 0 Render date: 2024-11-20T04:08:52.667Z Has data issue: false hasContentIssue false
  • English
  • Français

Partial characterization and time course analysis of Hymenolepis diminuta coproantigens

Published online by Cambridge University Press:  05 June 2009

J.C. Allan  and
P.S. Craig
J.C. Allan
Affiliation:
Department of Biological Sciences, University of Salford, Salford, M5 4WT, UK
P.S. Craig
Affiliation:
Department of Biological Sciences, University of Salford, Salford, M5 4WT, UK
Get access Rights & Permissions [Opens in a new window]

Abstract

An analysis of Hymenolepis diminuta specific antigens in infected rat faeces was carried out. Using a capture type antibody sandwich ELISA assay based on a hyperimmune rabbit anti-worm somatic antisera it was demonstrated that, although antigen was present in faeces before patency, the onset of egg production led to a sharp increase in the levels of parasite antigen in the faeces. Levels of antigen in host faeces were independent of worm burden. Parasite eggs did not contribute significantly to faecal antigen levels. Western blot analysis indicated a number of highly specific antigens at around Mr 69,000, Mr 37,000, Mr 50,000 and Mr 27,000 with a low molecular weight smear at between Mr 30,000 and Mr 34,000 present in the faeces of H. diminuta infected rats. Some cross reaction occurred with an antigen of around Mr 66,000 in the faeces of non H. diminuta infected rodents. Antibody activity against this antigen was removed by affinity adsorption of the antibody solution against normal rat faeces.

Type
Research Papers
Information
Journal of Helminthology , Volume 68 , Issue 2 , June 1994 , pp. 97 - 103
Copyright
Copyright © Cambridge University Press 1994

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Allan, J.C. & Craig, P.S. (1989) Coproantigens in gut tapeworm infections; Hymenolepis diminuta in rats. Parasitology Research 76, 68–73.CrossRefGoogle ScholarPubMed
Allan, J.C., Avila, G., Garcia Noval, J., Flisser, A. & Craig, P.S. (1990) Immunodiagnosis of taeniasis by coproantigen detection. Parasitology 101, 473–477.CrossRefGoogle ScholarPubMed
Allan, J.C., Craig, P.S., Garcia Noval, J., Mencos, F., Liu, D., Wang, Y., Wen, H., Zhou, P., Stringer, R., Rogan, M. & Zeyhle, E. (1992) Coproantigen detection for the immunodiagonsis of echinococcosis and taeniasis in dogs and humans. Parasitology 104, 347–355.CrossRefGoogle Scholar
Allan, J.C., Mencos, F., Garcia Noval, J., Sarti, E., Flisser, A., Wang, Y., Liu, D. & Craig, P.S. (1993) Dipstick dot ELISA for detection of Taenia coproantigens in humans. Parasitology 107, 79–85.CrossRefGoogle ScholarPubMed
Chandler, A.C. (1939) The effects of number and age of worms on development of primary and secondary infections with Hymenolepis diminuta in rats, and an investigation into the true nature of “premunition” in tapeworm infections. American Journal of Hygiene 29, 105–114.Google Scholar
Deplazes, P., Gottstein, B., Stingelin, Y. & Eckert, J. (1990) Detection of Taenia hydatigena coproantigens by ELISA in dogs. Veterinary Parasitology 36, 91–103.CrossRefGoogle ScholarPubMed
Deplazes, P., Eckert, J., Pawlowski, Z.S., Machowska, L. & Gottstein, B. (1991) An enzyme linked immunosorbent assay for diagnostic detection of Taenia saginata copro-antigens in humans. Transactions of the Royal Society of Tropical Medicine and Hygiene 85, 391–396.CrossRefGoogle ScholarPubMed
Deplazes, P., Gottstein, B., Eckert, J., Jenkins, D.J., Ewald, D. & Jiminez-Palacios, S. (1992) Detection of Echinococcus coproantigens by enzyme-linked immunosorbent assay in dogs, dingoes and foxes. Parasitology Research 78, 303–308.CrossRefGoogle ScholarPubMed
Green, E.L. (1986) Immunological detection of parasite antigen in faeces. Parasitology Today 2, 198–200.CrossRefGoogle ScholarPubMed
Hager, A. (1941) Effects of dietary modifications of host rats on the tapeworm Hymenolepis diminuta. Iowa State College Journal of Science 15, 127–153.Google Scholar
Hesselberg, C.A. & Andreassen, J. (1975) Some influences of population density on Hymenolepis diminuta in rats. Parasitology 71, 517–523.CrossRefGoogle ScholarPubMed
Keymer, A., Crompton, D.W.T. & Singhvi, A. (1983) Mannose and the “crowding effect” of Hymenolepis in rats. International Journal for Parasitology 13, 561–570.CrossRefGoogle ScholarPubMed
Lethbridge, R.C. (1980) The biology of the oncosphere of cyclophidean cestodes. Helminthological Abstracts 49, 59–72.Google Scholar
Maass, M., Delgado, E. & Knobloch, J. (1991) Detection of Taenia solium antigens in merthiolate-formalin preserved stool samples. Tropical Medicine and Parasitology 42, 112–114.Google ScholarPubMed
Maass, M., Delgado, E. & Knobloch, J. (1992) Isolation of an immunodiagnostic Taenia solium coproantigen. Tropical Medicine and Parasitology 43, 201–202.Google ScholarPubMed
Machnicka, B. & Krawczuk, S. (1988) Hymenolepis diminuta antigen: detection in faeces of rats. Bulletin of the Polish Academy of Science, Biological Science 36, 103–106.Google Scholar
Quinnell, R.J. (1988) Host age and the growth and fecundity of Hymenolepis diminuta in the rat. Journal of Helminthology 62, 158–162.CrossRefGoogle ScholarPubMed
Read, C.P. (1951) The “crowding effect” in tapeworm infections. Journal of Parasitology 37, 174–178.CrossRefGoogle Scholar
Ritchie, L.S. (1948) An ether sedimentation technique for routine stool examinations. Bulletin of the United States Army Medical Department 8, 326.Google ScholarPubMed
Roberts, L.S. (1961) The influence of population density on patterns and physiology of growth in Hymenolepis diminuta (Cestoda: Cyclophyllidea) in the definitive host. Experimental Parasitology 11, 332–371.CrossRefGoogle ScholarPubMed
Whitfield, P.J. (1979) The biology of parasitism. 277 pp. London, Arnold.Google Scholar
Wilson, M.B. & Nakane, P.K. (1978) Recent developments in the periodate method of conjugating horseradish peroxidase (HRPO) to antibodies. pp. 215–224, in Knapp, W., Holubar, K. & Wicks, F. (Eds) Immunofluorescence and related staining techiques. Amsterdam, Elsevier.Google Scholar