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New species of Parasaccocoelium (Haploporidae) and new genus Pseudohaplosplanchnus (Haplosplanchnidae) from mullet fish in the Far East of Russia and Vietnam: morphological and molecular data

Published online by Cambridge University Press:  14 May 2020

D.M. Atopkin Open the ORCID record for D.M. Atopkin [Opens in a new window] ,
V.V. Besprozvannykh ,
D.N. Ha ,
V.H. Nguyen  and
V.T. Nguyen
D.M. Atopkin*
Affiliation:
Federal Scientific Center of East Asia Terrestrial Biodiversity, Far Eastern Branch of Russian Academy of Sciences, Vladivostok690022, Russia Department of Cell Biology and Genetics, Far Eastern Federal University, Ajax-10 str., Vladivostok690051, Russia
V.V. Besprozvannykh
Affiliation:
Federal Scientific Center of East Asia Terrestrial Biodiversity, Far Eastern Branch of Russian Academy of Sciences, Vladivostok690022, Russia
D.N. Ha
Affiliation:
Institute of Ecology and Biological Resources, Vietnamese Academy of Sciences and Technology, Hanoi, Vietnam
V.H. Nguyen
Affiliation:
Institute of Ecology and Biological Resources, Vietnamese Academy of Sciences and Technology, Hanoi, Vietnam
V.T. Nguyen
Affiliation:
Hai Duong Medical Technical University, Vietnam
*
Author for correspondence: D.M. Atopkin, E-mail: [email protected]
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Abstract

A description and the molecular characterization of two new species in the Haploporidae and Haplosplanchnidae families are provided herein. Parasaccocoelium armatum n. sp. was collected from the intestine of a Mugil cephalus Linnaeus, 1758 from the Primorsky region, Russia, and Pseudohaplosplanchnus catbaensis n. g. n. sp. was collected from Moolgarda seheli (Forsskål, 1775) in the coastal waters of Cat Ba Island, Vietnam. The morphological features of P. armatum n. sp. closely resemble those of Parasaccocoelium polyovum, but these species differ from one another by hermaphroditic sac and vitellaria area length and by maximal egg size. The main difference between P. armatum n. sp. and P. polyovum is the presence of an armed hermaphroditic duct in the new species. Molecular data support the case for inclusion of the studied trematodes in P. armatum n. sp. Worms P. catbaensis n. g. n. sp. from the mullet from Vietnam are morphologically close to Haplosplanchnus (Haplosplosplanchninae). The only difference between P. catbaensis n. g. n. sp. and species of Haplosplanchnus is the presence of few (1–7) large eggs, measuring 135–142 × 92–104 μm, versus numerous small eggs with a maximal size of 75 × 50 μm. Phylogenetic analysis showed that there is a contradiction between the morphological similarity of the worms and their position in the Haplosplanchnidae system, based on the genetic data. Results of this study indicate that P. catbaensis n. g. n. sp. is genetically distant from other representatives of Haplosplanchnus, despite their morphological similarity. According to the molecular data, P. catbaensis n. g. n. sp. is close to Hymenocotta mulli Manter, 1961 (Hymenocottinae). However, these species are considerably different to each other morphologically. Molecular data argue for the possibility of establishing a new subfamily for P. catbaensis n. g. n. sp. However, considering earlier studies of Haplosplanchnidae, we support the view that creating new subfamilies within this family is unreasonable because of the lack of molecular data for most haplosplanchnid species, which are necessary to resolve the problematic systematics and phylogeny of this family.

Keywords

HaplosplanchnidaeHaploporidaeParasaccocoeliumPseudohaplosplanchnusmulletmolecular dataRussian Far EastVietnamphylogenetic relationships
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e154
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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References

Al-Bassel, DAML (1997) A review of the trematode genera Haplosplanchnus Looss, 1902 and Prohaplosplanchnus Tang and Lin, 1978 with redescription of three species from the mullet in Libya. Qatar University Science Journal 17(1), 133–142.Google Scholar
Andres, MJ, Curran, SS, Fayton, TJ, Pulis, EE and Overstreet, RM (2015) An additional genus and two additional species of Forticulcitinae (Digenea: Haploporidae). Folia Parasitologia 62, 025.Google Scholar
Atopkin, DM, Beloded, AY and Ngo, HD (2015) Molecular genetic characterization of the far eastern trematode Skrjabinolecithum spasskii Belous, 1954, (Digenea, Haploporidae), a parasite of mullets. Russian Journal of Molecular Biology 49, 373–379.Google Scholar
Atopkin, DM, Besprozvannykh, VV, Ha, DN, Nguyen, HV, Khamatova, AY and Vainutis, KS (2019) Correction to: Morphometric and molecular analyses of Carassotrema koreanum Park 1938 and Elonginurus mugilus Lu 1995 (Digenea: Haploporidae) Srivastava, 1937 from the Russian Far East and Vietnam. Parasitology Research 118(7), 2327.CrossRefGoogle ScholarPubMed
Besprozvannykh, VV, Atopkin, DM, Ermolenko, AV and Nikitenko, AY (2015) Restoration of the genus Parasaccocoelium Zhukov, 1971 (Digenea: Haploporidae) and a description of two new species from mugilid fish in the Far East of Russia. Journal of Helminthology 89(5), 565–576.CrossRefGoogle Scholar
Besprozvannykh, VV, Atopkin, DM, Ngo, HD, Ermolenko, AV, Ha, NV, Tang, NV and Nikitenko, AY (2016) Morphometric and molecular analyses of two digenean species from the mullet: Haplosplanchnus pachysomus (Eysenhardt, 1892) from Vietnam and Provitellotrema crenimugilis Pan, 1984 from the Russian southern Far East. Journal of Helminthology 90(2), 238–244.CrossRefGoogle ScholarPubMed
Besprozvannykh, VV, Atopkin, DM, Ermolenko, АV and Beloded, АY (2017a) Morphometric and molecular analyses for a new species Skrjabinolecithum pyriforme n. sp. (Digenea: Haploporidae) in mullet fish from the Primorsky Region, Russia. Journal of Helminthology 91, 625–632.CrossRefGoogle Scholar
Besprozvannykh, VV, Atopkin, DM, Ngo, HD, Ha, NV, Tang, NV and Beloded, АY (2017b) Morphometric and molecular analyses of two digenean species from the mullet: Skrjabinolecithum spinosum n. sp. from the Russian southern Far East and Unisaccus tonkini n. sp. from Vietnam. Journal of Helminthology 92, 713–724.CrossRefGoogle Scholar
Cribb, TH, Bray, RA, Littlewood, DTJ, Pichelin, S and Herniou, EA (2001) Relationships of the Digenea – evidence from molecules and morphology. pp. 186–193in Littlewood, DTJ and Bray, RA (Eds) Interrelationships of Platyhelminthes. London, Taylor & Francis.Google Scholar
Darriba, D, Taboada, GL, Doallo, R and Posada, D (2012) jModeltest2: more models, new heuristics and parallel computing. Nature Methods 9, 772.CrossRefGoogle Scholar
Huelsenbeck, JP, Ronquist, F, Nielsen, R and Bollback, JP (2001) Bayesian inference of phylogeny and its impact on evolutionary biology. Science 294, 2310–2314.CrossRefGoogle ScholarPubMed
Huston, DC, Cutmore, SC and Cribb, TH (2017) Molecular phylogeny of the Haplosplanchnata Olson, Cribb, Tkach, Bray and Littlewood, 2003, with a description of Schikhobalotrema huffmani n. sp. Acta Parasitologica 62(3), 502–512.CrossRefGoogle ScholarPubMed
Huston, DC, Cutmore, SC and Cribb, TH (2018) Trigonocephalotrema (Digenea: Haplosplanchnidae), a new genus for trematodes parasitising fishes of two Indo-West Pacific acanthurid genera. Invertebrate Systematics 32, 759–773.CrossRefGoogle Scholar
Kumar, S, Stecher, G and Tamura, K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33, 1870–1874.CrossRefGoogle ScholarPubMed
Littlewood, DTJ and Olson, PD (2001) Small subunit rDNA and the Platyhelminthes: signal, noise, conflict and compromise. pp. 262–278in Littlewood, DTJ and Bray, RA (Eds) Interrelationships of Platyhelminthes. London, Taylor & Francis.Google Scholar
Madhavi, R (2005) Superfamily Haplosplanchnoidea Poche, 1926. pp. 175–191in Jones, A, Bray, RA and Gibson, DI (Eds) Keys to the Trematoda, vol. 2. Wallingford, CAB International.CrossRefGoogle Scholar
Nahhas, FM, Rhodes, DY and Seeto, J (1997) Digenetic trematodes of marine fishes from Suva, Fiji, family Haplosplanchnidae Poche, 1926: description of new species, a review and an update. Suva, Fiji Islands, The University of the South Pacific, 93 pp.Google Scholar
Olson, PD, Cribb, TH, Tkach, VV, Bray, RA and Littlewood, DTJ (2003) Phylogeny and classification of the Digenea (Platyhelminthes: Trematoda). International Journal for Parasitology 33, 733–755.CrossRefGoogle Scholar
Overstreet, R and Curran, S (2005) Family Haploporidae Nicoll, 1914. pp.129-165 in Gibson, DI, Jones, A and Bray, RA (Eds) Keys to the Trematoda, vol. 2. Wallingford, CAB International.Google Scholar
Posada, D (2003) Using MODELTEST and PAUP* to select a model of nucleotide substitution. pp. 6.5.1–6.5.14 in Current Protocols in Bioinformatics, Baxevanis, et al. (eds). Ney York, John Wiley & Sons.Google Scholar
Pulis, E and Overstreet, R (2013) Review of haploporid (Trematoda) genera with ornate muscularisation in the region of the oral sucker, including four new species and a new genus. Systematic Parasitology 84, 167–191.CrossRefGoogle Scholar
Tkach, V, Pawlowski, J and Mariaux, J (2000) Phylogenetic analysis of the suborder Plagiorchiata (Platyhelminthes, Digenea) based on partial lsrDNA sequences. International Journal for Parasitology 30, 83–93.CrossRefGoogle ScholarPubMed
Tkach, VV, Littlewood, DTJ, Olson, PD, Kinsella, JM and Swiderski, Z (2003) Molecular phylogenetic analysis of the Microphalloidea Ward, 1901 (Trematoda: Digenea). Systematic Parasitology 56, 1–15.CrossRefGoogle Scholar
Truett, GE (2006) Preparation of genomic DNA from animal tissues. pp. 33–46in Kieleczawa, J (Ed) The DNA book: protocols and procedures for the modern molecular biology. Burlington, Massachusetts, Jones & Bartlett Publisher.Google Scholar
Zhukov, EV (1971) New trematodes of marine and freshwater fishes from the basins of the Japanese and Yellow seas. Parazitologiya 2, 155–161 (in Russian).Google Scholar