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A new species of Creptotrematina (Trematoda: Allocreadiidae) from characid fishes of Brazil: morphological and molecular data

Published online by Cambridge University Press:  16 June 2020

K.G. Alves Dias Open the ORCID record for K.G. Alves Dias [Opens in a new window] ,
G. Pérez-Ponce de León ,
A. de Almeida Camargo ,
M.I. Müller ,
R.J. da Silva ,
R. Kozlowiski de Azevedo  and
V.D. Abdallah
K.G. Alves Dias*
Affiliation:
Laboratório de Parasitologia de Animais Silvestres, Departamento de Parasitologia, Instituto de Biociências de Botucatu, Universidade Estadual Paulista ‘Júlio de Mesquita Filho’ (UNESP) – Campus Botucatu, Botucatu, São Paulo, Brazil
G. Pérez-Ponce de León
Affiliation:
Instituto de Biología, Universidad Nacional Autónoma de México (UNAM), Ciudad de México, Mexico Escuela Nacional de Estudios Superiores Unidad Mérida (ENES)-UNAM, Km 4.5 Carretera Mérida-Tetiz, Ucú, Yucatán, Mexico
A. de Almeida Camargo
Affiliation:
Laboratório de Parasitologia de Animais Silvestres, Departamento de Parasitologia, Instituto de Biociências de Botucatu, Universidade Estadual Paulista ‘Júlio de Mesquita Filho’ (UNESP) – Campus Botucatu, Botucatu, São Paulo, Brazil
M.I. Müller
Affiliation:
Programa de Pós-Graduação em Ecologia e Evolução, Universidade Federal de São Paulo, São Paulo, Brazil
R.J. da Silva
Affiliation:
Laboratório de Parasitologia de Animais Silvestres, Departamento de Parasitologia, Instituto de Biociências de Botucatu, Universidade Estadual Paulista ‘Júlio de Mesquita Filho’ (UNESP) – Campus Botucatu, Botucatu, São Paulo, Brazil
R. Kozlowiski de Azevedo
Affiliation:
Docente do Programa de Pós-graduação em Análise de Sistemas Ambientais, Centro Universitário CESMAC, Maceió, Alagoas, Brazil
V.D. Abdallah
Affiliation:
Docente do Programa de Pós-graduação em Análise de Sistemas Ambientais, Centro Universitário CESMAC, Maceió, Alagoas, Brazil
*
Author for correspondence: K.G. Alves Dias, E-mail: [email protected]
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Abstract

A new species of Creptotrematina Yamaguti, 1954 was collected from characid fishes, Astyanax fasciatus (Cuvier, 1819) and Astyanax lacustris Lucerna & Soares, 2016 from the Batalha River in the State of Sao Paulo, Brazil. The new species most closely resembles Creptotrematina aguirrepequenoi, but differs by the elongated shape of vitelline follicles, the extension of these follicles in the posterior end of body and the fact that they are not confluent. The morphological differences were confirmed through molecular data. Three specimens were sequenced, and molecular analyses were based on the internal transcribed spacers 2 and D1–D3 domains of the 28S ribosomal RNA gene. The obtained topologies showed the new species as a sister taxon of C. aguirrepequenoi, a species originally described from Astyanax mexicanus in Mexico, and later found in Astyanax aeneus in Costa Rica. Isolates of the new species are reciprocally monophyletic, and genetic distance values are similar to those observed in other species pairs within Allocreadiidae. These findings corroborate that the genus Creptotrematina is mostly a parasite of characids, and widely extended across the Americas, with representative species occurring between Argentina and northern Mexico.

Keywords

ITS rDNA28S rDNASouth Americafreshwater fishesintegrative taxonomydigeneans
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e163
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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