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Motility of Fasciola hepatica miracidia assessed with a computer-assisted sperm analyser

Published online by Cambridge University Press:  23 April 2014

A. Villa-Mancera ,
A. Reynoso-Palomar ,
J. Olivares-Pérez ,
S. Ortega-Vargas ,
I. Cruz-Mendoza  and
H. Quiroz-Romero
A. Villa-Mancera*
Affiliation:
Facultad de Medicina Veterinaria y Zootecnia, Benemérita Universidad Autónoma de Puebla, Tecamachalco Puebla, México
A. Reynoso-Palomar
Affiliation:
Facultad de Medicina Veterinaria y Zootecnia, Benemérita Universidad Autónoma de Puebla, Tecamachalco Puebla, México
J. Olivares-Pérez
Affiliation:
Unidad Académica de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Guerrero, Ciudad Altamirano, Guerrero, México
S. Ortega-Vargas
Affiliation:
Departamento de Parasitología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, México, D.F.México
I. Cruz-Mendoza
Affiliation:
Departamento de Parasitología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, México, D.F.México
H. Quiroz-Romero
Affiliation:
Departamento de Parasitología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, México, D.F.México
*
* E-mail: [email protected]
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Abstract

The motility parameters of Fasciola hepatica miracidia were assessed at different temperatures and times post-hatching using computer-assisted sperm analysis. Eggs were incubated at 22°C or 25°C for 14 days. Five motion parameters were evaluated at different incubation temperatures up to 10 h post-hatching. No differences were observed in the percentage that hatched after incubation at the two different temperatures. However, the straight-line velocity of miracidia following incubation at 22°C was significantly different from that observed at 25°C (P< 0.01). All miracidium motion parameters at different post-hatching temperatures showed an overall decrease at the end of the experiment. Those miracidia hatching from eggs incubated at 25°C had a higher velocity of 1673.3 μm/s compared with 1553.3 μm/s at 22°C. Velocity parameters increased as the post-hatching temperature increased from 22°C to 37°C.

Type
Research Papers
Information
Journal of Helminthology , Volume 89 , Issue 4 , July 2015 , pp. 453 - 457
Copyright
Copyright © Cambridge University Press 2014 

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