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Morphometric variability of Schistosoma intercalatum eggs: a diagnostic dilemma

Published online by Cambridge University Press:  05 June 2009

J. Almeda ,
C. Ascaso ,
G. A. Marçal ,
M. Corachan ,
V. R. Southgate  and
D. Rollinson
J. Almeda*
Affiliation:
Unidad de Epidemiologia y Bioestadistica, Hospital Clinic, Universidad de Barcelona, Spain
C. Ascaso
Affiliation:
Unidad de Epidemiologia y Bioestadistica, Hospital Clinic, Universidad de Barcelona, Spain
G. A. Marçal
Affiliation:
Laboratorio Central, Hospital Ayres Menezes, Ministerio da Saude, Republica de São Tomé and Principe
M. Corachan
Affiliation:
Seccion de Medicina Tropical, Hospital Clinic, Universidad de Barcelona, Spain
V. R. Southgate
Affiliation:
Department of Zoology, The Natural History Museum, Cromwell Road, London, SW7 5BD, UK
D. Rollinson
Affiliation:
Department of Zoology, The Natural History Museum, Cromwell Road, London, SW7 5BD, UK
*
*Unidad de Epidemiologia y Bioestadistica, Fundacio Clinic per a la Recerca Biomedica, Villarroel 170, 08036 Barcelona, Spain.
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Abstract

Variability of Schistosoma intercalatum eggs in shape and size, and their similarity to those of S. haematobium presented a problem of species identification when egg morphology was the diagnostic criterion used in a study of human schistosomiasis conducted on São Tomé and Principe. More than 2500 egg measurements were obtained by light micoscopy to gather data relating to size variability of S. intercalatum eggs, to evaluate whether factors such as age of host, sex of host and intensity of infection are correlated with variability, and the data were compared with previously published measurements on different isolates and strains of S. intercalatum: the range in length (104–203 μm) embraces most of the measurements reported in other studies of S. intercalatum eggs. There was no correlation either between age and sex of the host, or intensity of infection with variability of egg size. Comparison between measurements of the eggs of S. haematobium, S. intercalatum and S. bovis eggs are presented.

Type
Research Papers
Information
Journal of Helminthology , Volume 70 , Issue 2 , June 1996 , pp. 97 - 102
Copyright
Copyright © Cambridge University Press 1996

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References

Almeda, J., Corachan, M., Sousa, A., Ascaso, C., Carvalho, J.M., Rollinson, D. & Southgate, V.R. (1994) Schistosomiasis in the Republic of São Tomé and Principe: human studies. Transactions of the Royal Society of Tropical Medicine and Hygiene 88, 406–409.CrossRefGoogle ScholarPubMed
Arene, F.O.I., Ukpeibo, E.T & Nwanze, E.A. (1989) Studies on schistosomiasis in the Niger Delta: Schistosoma intercalatum in the urban City of Port Harcourt, Nigeria. Public Health 103, 295–301.CrossRefGoogle ScholarPubMed
Bjorneboe, A. & Frandsen, F. (1979) A comparison of the characteristics of two strains of Schistosoma intercalatum Fisher, 1934 in mice. Journal of Helminthology 53, 195–203.CrossRefGoogle ScholarPubMed
Brown, D.S., Sarfati, C., Southgate, V.R., Ross, G.C. & Knowles, R.J. (1984) Observations on Schistosoma intercalatum in Southeast Gabon. Zeitschrift für Parasitenkunde 70, 243–253.CrossRefGoogle Scholar
Clapier, P.N. (1920) Contribution à l'ètude de la repartition des bilharzioses en Afrique Equatorials Francaise. Bulletin de la Société de Pathologi Exotique 13, 804–809.Google Scholar
Corachan, M., Ruiz, L., Valls, M.E. & Gascon, J. (1992) Schistosomiasis and the Dogon country (Mali). American Journal of Tropical Medicine and Hygiene 47, 6–9.Google Scholar
Deniau, M., Eben-Moussi, E., Koki-Ndombo, P., Same-Ekobo, A. & Ripert, C. (1981) Sensitivity of a strain of Schistosoma intercalatum from Loum (Cameroon) and its natural hybrid to treatment with praziquantel. Preliminary results. Arzneimittelforschung 31, 589–591.Google ScholarPubMed
De Clercq, D., Rollinson, D., Diarra, A., Sacko, M., Coulibaly, G., Landoure, G., Traore, M., Southgate, V.R., Kaukas, A. & Vercruysse, J. (1994) Schistosomiasis in Dogon country Mali: identification and prevalence of the species responsible for infection in the local community. Transactions of the Royal Society of Tropical Medicine and Hygiene 88, 653–656.CrossRefGoogle ScholarPubMed
Doumenge, J.P., Mott, K.E., Cheung, C., Villenave, D., Chapuis, O., Perrin, M.F. & Reaud-Thomas, G. (1987) Atlas of the global distribution of schistosomiasis. Presses Universitaires de Bordeaux, 1: 151.Google Scholar
Fain, A. (1952) Description de la cercaire de Schistosoma intercalatum Fisher, 1934 et d'une nouvefle xiphidiocercaire du groupe Ornatae (sous-groupe Prima). Annales de la Société Belge Médecine Tropicale 32, 433–441.Google Scholar
Fisher, A.C. (1934) A study of the schistosomiasis of the Stanleyville District of the Belgian Congo. Transactions of the Royal Society of Tropical Medicine and Hygiene 28, 277–306.Google Scholar
Frandsen, F. (1978) Hybridisation between strains of Schistosoma intercalatum Fisher, 1934 from Cameroun and Zaire, journal of Helminthology 52, 11–22.CrossRefGoogle ScholarPubMed
Gracio, M. A. (1988) The genus Bulinus in São Tomé e Principe: first record and contribution to the life history. Journal of Medical and Applied Malacology 1, 165–172.Google Scholar
Kirkwood, B.R. (1988). Essentials of medical statistics. 148pp. Oxford, Blackwell Scientific Publications Ltd.Google Scholar
Lagrange, E. (1920) Sur un cas de bilharziose intestinale à Schistosoma haematobium. Bulletin de la Société de Pathologie Exotique 8, 28–29.Google Scholar
Muller, R.L. & Taylor, M.G. (1972) On the specific use of the Ziehl Neelson technique for the specific identification of schistosome eggs. Journal of Helminthology 46, 139–142.Google Scholar
Pages, J.R. & Théron, A. (1989) Schistosoma intercalatum: Variations morphologiques et biometriques des oeufs en relation avec la localization chez l'hote definitif et l'origine geographique du parasite (Cameroun et Zaire). Annales Parasitologie Humaine et Comparée 64, 208–216.Google Scholar
Pampiglione, S., Visconti, S. & Pezzino, G. (1987) Human intestinal parasites in Subsaharan Africa. II. São Tomè and Principe. Parassitologia 29, 15–25.Google Scholar
Pitchford, R.J. (1965) Differences in the egg morphology and certain biological characteristics of some African and Middle Eastern schistosomes, genus Schistosoma with terminal spined eggs. Bulletin of the World Health Organization 32, 105–120.Google ScholarPubMed
Ratard, R.C. & Greer, G.J. (1991) A new focus of Schistosoma haematobium/S. intercalatum hybrid in Cameroon. American Journal of Tropical Medicine and Hygiene 45, 332–338.Google Scholar
Rollinson, D. & Southgate, V.R. (1987) The genus Schistosoma: A taxonomic appraisal. pp. 16–26in Rollinson, D. & Simpson, A.J.G. (Eds) The biology of schistosomes. From genes to latrines. London, Academic Press Limited.Google Scholar
Rollinson, D., Vercruysse, J., Southgate, V.R., Moore, P.J., Ross, G.C., Walker, T.K. & Knowles, R.J. (1987) Observations on human and animal schistosomiasis in Senegal, pp. 119–132in Geerts, S., Kumar, V. & Brandt, J.(Eds) Helminth zoonoses. Martinus Nijhoff, Dordrecht.Google Scholar
Romero, M, Corachan, M. & Luis, M. (1989) Schistosomiasis in São Tomé. A pilot study. Transactions of the Royal Society of Tropical Medicine and Hygiene 83, 81–82.Google Scholar
Sentís, J., Ascaso, C., Vallès, A. & Canela, J. (1992) Teoría del muestreo Cap.5. Estimatión de parámetros. pp. 111–116in Licenciatura Biostadística.Cientificas y Técnicas, S.A. Barcelona.Google Scholar
Southgate, V.R., Rollinson, D., Ross, G.C. & Knowles, R.J. (1980) Observations on an isolate of Schistosoma bovis from Tanzania. Zeitschtift für Parasitenkunde 63, 241–249.Google Scholar
Southgate, V.R., Rollinson, D., Kaukas, A., Almeda, J., Sousa, A.M., Castro, F., Soares, E. & Corachan, M. (1994) Schistosomiasis in the Republic of São Tomé and Principe: characterization of Schistosoma intercalatum. Transactions of the Royal Society of Tropical Medicine and Hygiene 88, 479–486.Google Scholar
Taylor, M.G. (1970) Hybridization experiments on five species of African schistosomes. Journal of Helminthology 44, 253–314.Google Scholar
Wijk, H.B. van (1969a) Infection with Schistosoma intercalatum in Mungo Department, Cameroun. Tropical and Geographical Medicine 21, 362–374.Google Scholar
Wijk, H.B. van (1969b) Schistosoma intercalatum—infection in school-children of Loum, Cameroun. Tropical and Geographical Medicine 21, 375–382.Google Scholar
World Health Organization (1993) The control of schistosomiasis. Second report of the WHO Expert Committee. World Health Organization Technical Report Series 830. Geneva: WHO 86 pp.Google Scholar
Wright, C.A., Southgate, V.R. & Knowles, R.J. (1972). What is Schistosoma intercalatum Fisher, 1934? Transactions of the Royal Society of Tropical Medicine and Hygiene 66, 28–64.CrossRefGoogle ScholarPubMed