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Morphological changes of larval Echinococcus multilocularis in mice treated with albendazole or mebendazole

Published online by Cambridge University Press:  05 June 2009

K. Nakaya*
Affiliation:
Animal Laboratory for Medical Research, Asahikawa Medical College, 3-11 Nishikagura 4-sen 5-go, Asahikawa, Hokkaido 078-8510, Japan
Y. Oomori
Affiliation:
Department of Anatomy, Asahikawa Medical College, 3-11 Nishikagura 4-sen 5-go, Asahikawa, Hokkaido 078-8510, Japan
H. Kutsumi
Affiliation:
Department of Parasitology, Asahikawa Medical College, 3-11 Nishikagura 4-sen 5-go, Asahikawa, Hokkaido 078-8510, Japan
M. Nakao
Affiliation:
Department of Parasitology, Asahikawa Medical College, 3-11 Nishikagura 4-sen 5-go, Asahikawa, Hokkaido 078-8510, Japan
*
*Fax: +81 166 68 2429 E-mail: [email protected]

Abstract

Using DBA/2J mice, tissue homogenates of larval Echinococcus multilocularis were injected into the mesenteric veins to generate the liver infection. Mice were treated with either albendazole or mebendazole for prolonged periods to examine the morphological changes of the metacestode. Albendazole induced disorganization of both laminated and germinal layers and suppressed the maturation of vesicles. Amorphous but loosely laminated PAS-positive material was observed inside the damaged vesicles, although new vesicles slightly developed inside or outside of the damaged ones. Active proliferation of vesicles occurred after treatment with albendazole was terminated. Hydatid cysts were more severely damaged in mice treated with mebendazole and new vesicles did not develop around the damaged ones. Also, hydatid cysts reappeared after treatment with mebendazole was terminated. These results indicate that these drugs do not eliminate larval E. multilocularis in the long-term, but mebendazole has a higher suppressive effect on multivesiculation than albendazole.

Type
Research Papers
Copyright
Copyright © Cambridge University Press 1998

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References

Campbell, W.C. & Blair, S. (1974) Treatment of the cystic stage of Taenia crassiceps and Echinococcus multilocularis in laboratory animals. Journal of Pamsitology 60, 10531054.Google Scholar
Delatour, P., Parish, R.C. & Gyurik, R.J. (1981) Albendazole: a comparison of relay embryotoxicity with embryotoxicity of individual metabolites. Annales de Recherches Veterinaires 12, 159167.Google Scholar
Eckert, J. & Burkhardt, B. (1980) Chemotherapy of experimental echinococcosis. Acta Tropica 37, 297300.Google Scholar
Heath, D.D. & Chevis, R.A.F. (1974) Mebendazole and hydatid cysts. Lancet 2, 218291.CrossRefGoogle ScholarPubMed
Inaoka, T., Nakao, M., Ohnishi, K. & Kutsumi, H. (1987) Experimental therapy in Chinese hamsters and rats infected with larval Echinococcus multilocularis by using mebendazole, albendazole and ivermectin with brief review of chemotherapy of human multilocular echinococcosis. Hokkaido Igaku Zasshi 62, 5467.Google Scholar
Kanazawa, T., Kagei, N., Asahi, H. & Mochida, K. (1994) Effects of mebendazole and albendazole on secondary alveolar hydatid disease in Mongolian gerbils with special reference to the riming of treatment. Japanese Journal of Parasitology 43, 305307.Google Scholar
Krotov, A.I., Cherniaeva, A.I., Kovalenko, F.P., Baiandina, D.G., Budanova, I.S., Kuznetsova, O.E. & Voskoboinik, L.V. (1974) Experimental therapy of alveococcosis. II. Effectiveness of some anti-nematode preparations in alveococcosis of laboratory animals. Meditsinskaya Parazitologiya i Parazitarnye Bolezni 43, 314319.Google Scholar
Laclette, J.P., Guerra, G. & Zetina, C. (1980) Inhibition of tubulin polymerization by mebendazole. Biochemical and Biophysical Research Communications 92, 412423.CrossRefGoogle ScholarPubMed
Nakao, M., Nakaya, K. & Kutsumi, H. (1990) Murine model for hepatic hydatid disease without biohazard. Japanese Journal of Parasitology 39, 296298.Google Scholar
Nakaya, K., Nakao, M. & Ito, A. (1997) Echinococcus multilocularis: mouse strain difference in hydatid development. Journal of Helminthology 71, 5356CrossRefGoogle ScholarPubMed
Richards, K.S., Morris, D.L. & Taylor, D.H. (1989) Echinococcus multilocularis: ultrastructural effect of in vivo albendazole and praziquantel therapy, singly and in combination. Annals of Tropical Medicine and Parasitology 83, 479484.Google Scholar
Schantz, P.M., Brandt, F.H., Dickinson, C.M., Allen, C.R., Roberts, J.M. & Eberhard, M.L. (1990) Effects of albendazole on Echinococcus multilocularis infection in the Mongolian jird. Journal of Infectious Diseases 162, 14031407.Google Scholar
Taylor, D.H., Morris, D.L., Reffin, D. & Richards, K.S. (1989) Comparison of albendazole, mebendazole and praziquantel chemotherapy of Echinococcus multilocularis in a gerbil model. Gut 30, 14011405.Google Scholar
Thompson, R.C.A. (1976) The development of brood capsules and protoscolices in secondary hydatid cysts of Echinococcus granulosus. Zeitschrift für Parasitenkunde 51, 3136.CrossRefGoogle ScholarPubMed
Vanparijs, O. (1990) Chemotherapy of experimental Echinococcus multilocularis in jirds. Parasitology Research 76, 238340.Google Scholar
Whittaker, S.G. & Faustman, E.M. (1992) Effects of benzimidazole analogs on cultures of differentiating rodent embryonic cells. Toxicology and Applied Pharmacology 113, 144151.Google Scholar
WHO Informal Working Group on Echinococcosis (1996) Guidelines for treatment of cystic and alveolar echinococcosis in humans. Bulletin of the World Health Organization 74, 231242.Google Scholar