Hostname: page-component-586b7cd67f-rcrh6 Total loading time: 0 Render date: 2024-11-23T09:02:10.964Z Has data issue: false hasContentIssue false
  • English
  • Français

Morphological and molecular characterization of two new species of the genus Aporcelinus Andrássy, 2009 (Nematoda, Dorylaimida, Aporcelaimidae) from the USA, with new insights on the phylogeny of the genus

Published online by Cambridge University Press:  11 December 2018

S. Álvarez-Ortega Open the ORCID record for S. Álvarez-Ortega [Opens in a new window] ,
S.A. Subbotin  and
R. Peña-Santiago
S. Álvarez-Ortega*
Affiliation:
Departamento de Biología y Geología, Física y Química Inorgánica, Universidad Rey Juan Carlos, Campus de Móstoles, 28933-Madrid, España Plant Pest Diagnostics Center, California Department of Food and Agriculture, 3294 Meadowview Road, Sacramento, CA 95832-1448, USA
S.A. Subbotin
Affiliation:
Plant Pest Diagnostics Center, California Department of Food and Agriculture, 3294 Meadowview Road, Sacramento, CA 95832-1448, USA
R. Peña-Santiago
Affiliation:
Departamento de Biología Animal, Biología Vegetal y Ecología, Universidad de Jaén, Campus ‘Las Lagunillas’ s/n, Edificio B3, 23071- Jaén, Spain
*
Author for correspondence: S. Álvarez-Ortega, E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Two new species of the genus Aporcelinus from the USA are described and illustrated. Aporcelinus floridensis sp. n. is characterized by its 1.12–1.52 mm long body, lip region offset by marked constriction and 14.5–17.0 μm broad with perioral liplets, odontostyle 16.5–20.0 μm at its ventral side and 1.1–1.2 times the lip region diameter, neck 316–395 μm long, pharyngeal expansion occupying 43–48% of total neck length, uterus simple and 33–56 μm long or 0.8–1.2 times the corresponding body diameter, V = 48–54, female tail conical (36–49 μm long, c = 27–41, c’ = 1.2–2.0) with finely rounded terminus and no hyaline portion, and male absent. Aporcelinus paolae sp. n. is characterized by its 1.29–1.80 mm long body, lip region offset by marked constriction and 14–16 μm broad, odontostyle 15–17 μm at its ventral side and 1.0–1.1 times the lip region diameter, neck 314–397 μm long, pharyngeal expansion occupying 43–53% of total neck length, uterus tripartite and 128–164 μm long or 2.6–3.6 times the corresponding body diameter, V = 53–57, female tail conical (30–39 μm long, c = 40–51, c’ = 1.1–1.3) with finely rounded terminus and variably re-curved dorsad, male tail conical (27–36 μm, c = 39–59, c’ = 0.9–1.2), ventrally straight and dorsally convex, spicules 48–54 μm long, and 7–9 irregularly spaced ventromedian supplements lacking hiatus. The analyses of the D2-D3 expansion segments of 28S rRNA (LSU) gene sequences of the two new species confirmed the monophyly of the genus, based upon currently available data, showing a close relationship between the genera Aporcelinus and Makatinus, and justified the placement of Aporcelaimellus, Makatinus and Aporcelinus under the subfamily Aporcelaimellinae.

Keywords

Bayesian inferencedescriptionD2-D3 expansion segmentsLSU ribosomal RNA genemaximum likelihoodmolecularmorphologymorphometricsSEMtaxonomy
Type
Research Paper
Information
Journal of Helminthology , Volume 94 , 2020 , e22
Copyright
Copyright © Cambridge University Press 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Álvarez-Ortega, S and Peña-Santiago, R (2013) Taxonomy of the genus Aporcelaimellus Heyns, 1965 (Nematoda, Dorylaimida, Aporcelaimidae). Zootaxa 3669, 243–260. http://dx.doi.org/10.11646/zootaxa.3669.3.3Google Scholar
Álvarez-Ortega, S and Peña-Santiago, R (2016) Aporcella charidemiensis sp. n. (Dorylaimida: Aporcelaimidae) from the southern Iberian Peninsula, with comments on the phylogeny of the genus. Nematology 18, 811–821. http://dx.doi.org/10.1163/15685411-00002995Google Scholar
Álvarez-Ortega, S, Subbotin, SA and Peña-Santiago, R (2013a) Morphological and molecular characterisation of Aporcelaimellus simplex (Thorne & Swanger, 1936) Loof & Coomans, 1970 and a new concept for the genus Aporcella Andrássy, 2002 (Dorylaimida, Aporcelaimidae). Nematology 15, 165–178. http://dx.doi.org/10.1163/156854112X651320Google Scholar
Álvarez-Ortega, S, Subbotin, SA and Peña-Santiago, R (2013b) Morphological and molecular characterisation of Californian species of the genus Aporcelaimellus Heyns, 1965 (Dorylaimida: Aporcelaimidae). Nematology 15, 431–439. http://dx.doi.org/10.1163/15685411-00002691Google Scholar
Andrássy, I (1986) The genus Eudorylaimus Andrássy, 1959 and the present status of its species (Nematoda: Qudsianematidae). Opuscula Zoologica Budapestinensis 22, 1–42.Google Scholar
Andrássy, I (1998) Nematodes in the sixth continent. Journal of Nematode Morphology and Systematics 1, 107–186.Google Scholar
Andrássy, I (2004) Two new species of Aporcelaimellus Heyns, 1965 (Nematoda: Dorylaimida) from the tropics. Acta Zoologica Academiae Scientiarum Hungaricae 50, 97–107.Google Scholar
Andrássy, I (2009a) Aporcelinus, a new genus of aporcelaimoid nematodes (Dorylaimida), and its species. International Journal of Nematology 19, 121–136.Google Scholar
Andrássy, I (2009b) Another species of the genus Aporcelinus Andrássy, 2009 (Nematoda: Dorylaimida). Opuscula Zoologica Budapestinensis 40, 99–102.Google Scholar
Andrássy, I (2012) Two new species of the family Aporcelaimidae (Nematoda: Dorylaimida). Genus 23, 189–199.Google Scholar
Barker, KR (1985) Nematode extraction and bioassays. In Barker, KR, Carter, CC and Sasser, JN (eds), An Advanced Treatise on Meloidogyne, Volume II. Methodology. Raleigh, NC: North Carolina State University, pp. 19–35.Google Scholar
Cobb, NA (1920) One hundred new nemas. (Type species of 100 new genera). Contributions to a Science of Nematology 9, 217–243.Google Scholar
Darriba, D et al. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9, 772.Google Scholar
Heyns, J (1965) On the morphology and taxonomy of the Aporcelaimidae, a new family of dorylaimoid nematodes. Entomology Memoirs, Department of Agricultural Technical Services, Republic of South Africa 10, 1–51.Google Scholar
Holterman, M et al. (2008) A ribosomal DNA-based framework for the detection and quantification of stress-sensitive nematode families in terrestrial habitats. Molecular Ecology Resources 8, 23–34. http://dx.doi.org/10.1111/j.1471-8286.2007.01963.xGoogle Scholar
Jairajpuri, MS (1965) Qudsianema amabilis n. gen., n. sp. (Nematoda: Dorylaimoidea) from India. Proceedings of the Helminthological Society of Washington 31, 59–64.Google Scholar
Loof, PAA and Coomans, A (1970) On the development and location of the oesophageal gland nuclei in Dorylaimina. Proceedings of the IX International Nematology Symposium (Warsaw, Poland, 1967), pp. 79–161.Google Scholar
Miller, M, Pfeiffer, W and Schwartz, T (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. Proceedings of the Gateway Computing Environments Workshop (GCE), 2010 (USA), pp. 1–8.Google Scholar
Nguyen, TAD et al. (2016a) Two new species of the genus Aporcelinus Andrássy, 2009 (Nematoda, Dorylaimida, Aporcelaimidae) from Vietnam. Zootaxa 4103, 550–560. http://dx.doi.org/10.11646/zootaxa.4103.6.5Google Scholar
Nguyen, TAD et al. (2016b) A third new species of Aporcelinus Andrássy, 2009 (Dorylaimida, Aporcelaimidae) from Vietnam, with the first SEM study of a representative of the genus. Journal of Nematology 48, 104–108. http://journals.fcla.edu/jon/article/view/88235Google Scholar
Nguyen, TAD et al. (2017) Two known species of Aporcelinus Andrássy, 2009 (Dorylaimida: Aporcelaimidae) from Vietnam, with the first molecular study of the genus. Nematology 19, 853–868. http://dx.doi.org/10.1163/15685411-00003092Google Scholar
Nicholas, KB, Nicholas, HB Jr and Deerfield II, DW (1997) GeneDoc: analysis and visualization of genetic variation. EMBnet News 4, 1–14.Google Scholar
Peña-Santiago, R and Abolafia, J (2016) On the identity of Aporcelinus granuliferus (Cobb, 1893) Andrássy, 2009 and its taxonomic consequences. Nematology 18, 999–1014. http://dx.doi.org/10.1163/15685411-00003011Google Scholar
Peña-Santiago, R, Abolafia, J and Álvarez-Ortega, S (2014) New proposal for a detailed description of the dorylaim spicule (Nematoda: Dorylaimida). Nematology 16, 1091–1095. http://dx.doi.org/10.1163/15685411-00002834Google Scholar
Ronquist, F et al. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61, 539–542.Google Scholar
Siddiqi, MR (1964) Studies on Discolaimus spp. (Nematoda: Dorylaimidae) from India. Zeitschrift für Zoologische Systematik und Evolutionsforschung 2, 174–184. http://dx.doi.org/10.1111/j.1439-0469.1964.tb00720.xGoogle Scholar
Stamatakis, A (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30, 1312–1313. https://doi.org/10.1093/bioinformatics/btu033Google Scholar
Subbotin, SA et al. (2006) Phylogenetic analysis of Tylenchida Thorne, 1949 as inferred from D2 and D3 expansion fragments of the 28S rRNA gene sequences. Nematology 8, 455–474. http://dx.doi.org/10.1163/156854106778493420Google Scholar
Thompson, JD et al. (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25, 4876–4882. http://dx.doi.org/10.1093/nar/25.24.4876Google Scholar
Thorne, G (1939) A monograph of the nematodes of the superfamily Dorylaimoidea. Capita Zoologica 8, 1–261.Google Scholar
Thorne, G and Swanger, HH (1936) A monograph of the nematode genera Dorylaimus Dujardin, Aporcelaimus n. g., Dorylaimoides n. g. and Pungentus n. g. Capita Zoologica 6, 1–223.Google Scholar
Varela-Benavides, I and Peña-Santiago, R (2018) A new species of the genus Aporcelinus Andrássy, 2009 (Nematoda, Dorylaimida, Aporcelaimidae) from Costa Rica. Zootaxa 4450, 489–494. http://dx.doi.org/10.11646/zootaxa.4450.4.7Google Scholar
Vinciguerra, MT, Orselli, L and Clausi, M (2014) One new and two known species of Aporcelinus Andrássy, 2009 and a new species of Coomansinema Ahmad & Jairajpuri, 1989 (Nematoda: Dorylaimida). Nematology 16, 303–322.Google Scholar
Zhao, CD and Zhao, HH (2010) A new species of the genus Aporcelaimellus Heyns, 1965 – Aporcelaimellus jiaoanensis sp. nov. (Nematoda, Dorylaimida, Aporcelaimidae). Acta Zootaxonomica Sinica 35, 876–879. (In Chinese)Google Scholar