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Morphological and molecular characterization of selected species of Hysterothylacium (Nematoda: Raphidascarididae) from marine fish in Iraqi waters

Published online by Cambridge University Press:  02 March 2017

M. Ghadam ,
M. Banaii ,
E.T. Mohammed ,
J. Suthar  and
S. Shamsi
M. Ghadam
Affiliation:
School of Animal and Veterinary Sciences, Charles Sturt University, Wagga Wagga, New South Wales 2678, Australia Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
M. Banaii
Affiliation:
Marine Fisheries, Marine Science Center, University of Basrah, Iraq
E.T. Mohammed
Affiliation:
Marine Fisheries, Marine Science Center, University of Basrah, Iraq
J. Suthar
Affiliation:
School of Animal and Veterinary Sciences, Charles Sturt University, Wagga Wagga, New South Wales 2678, Australia
S. Shamsi*
Affiliation:
School of Animal and Veterinary Sciences, Charles Sturt University, Wagga Wagga, New South Wales 2678, Australia
*
*Fax: +61 2 69334887 E-mail: [email protected]
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Abstract

Hysterothylacium species are perhaps the most abundant and diverse group of marine ascaridoids; however, their life cycle and specific identification in larval stages in many parts of the world, particularly in Iraqi marine waters, have not been completely understood. In this study three members of the genus Hysterothylacium collected from Khor Abdulla in Iraq are morphologically described, genetically characterized and their relationship with other closely related taxa are compared and discussed. A new Hysterothylacium larval type in the fourth stage of development is described, and morphological and molecular evidence (based on the sequences of internal transcribed spacers) are provided for its distinction from previously known fourth-stage Hysterothylacium larval types. Based on the sequence data it is suggested that the new larval type, which herein was assigned as Hysterothylacium larval type XVI, is H. persicum which was previously reported from the close proximity in Bandar Abbas, Iran. In addition, two other taxa, including Hysterothylacium larval type XV and H. reliquens, have been found in the present study, for which new hosts are reported. This study provides some insights into the taxonomy and systematics of these parasites, not only in this region but also for similar studies elsewhere.

Type
Research Papers
Information
Journal of Helminthology , Volume 92 , Issue 1 , January 2018 , pp. 116 - 124
Copyright
Copyright © Cambridge University Press 2017 

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References

Ali, A., Mhaisen, F. & Khamees, N. (2014) Checklists of nematodes of freshwater and marine fishes of Basrah Province, Iraq. Mesopotamian Journal of Marine Sciences 29, 71–96.Google Scholar
Deardorff, T.L. & Overstreet, R.M. (1981) Review of Hysterothylacium and Iheringascaris (both previously = Thynnascaris) (Nematoda: Anisakidae) from the northern Gulf of Mexico. Proceedings of the Biological Society of Washington 93, 1035–1079.Google Scholar
Froese, R. & Pauly, D. (2016) FishBase. World Wide Web electronic publication version (10/2016). Available at www.fishbase.org (accessed 10 November 2016).Google Scholar
Li, L., Zhao, W.T., Guo, Y.N. & Zhang, L.P. (2016) Nematode parasites infecting the starry batfish Halieutaea stellata (Vahl) (Lophiiformes: Ogcocephalidae) from the East and South China Sea. Journal of Fish Diseases 39, 515–529.Google Scholar
Norris, D.E. & Overstreet, R.M. (1975) Thynnascaris reliquens sp. n. and Thynnascaris habena (Linton, 1900) (Nematoda: Ascaridoidea) from fishes in Northern gulf of Mexico and eastern US seaboard. Journal of Parasitology 61, 330–336.Google Scholar
Petter, A.J. & Sey, O. (1997) Nematode parasites of marine fishes from Kuwait, with a description of Cucullanus trachinoti n. sp. from Trachinotus blochi . Zoosystema 19, 35–59.Google Scholar
Shamsi, S. (2016) Morphometric and molecular descriptions of three new species of Hysterothylacium (Nematoda: Raphidascarididae) from Australian marine fish. Journal of Helminthology. doi: 10.1017/S0022149X16000596.Google Scholar
Shamsi, S., Gasser, R., Beveridge, I. & Shabani, A.A. (2008) Contracaecum pyripapillatum n. sp. and a description of C. multipapillatum (von Drasche, 1882) from the Australian pelican, Pelecanus conspicillatus . Parasitology Research 103, 1031–1039.Google Scholar
Shamsi, S., Norman, R., Gasser, R. & Beveridge, I. (2009a) Genetic and morphological evidences for the existence of sibling species within Contracaecum rudolphii (Hartwich, 1964) (Nematoda: Anisakidae) in Australia. Parasitology Research 105, 529–538.Google Scholar
Shamsi, S., Norman, R., Gasser, R. & Beveridge, I. (2009b) Redescription and genetic characterization of selected Contracaecum spp. (Nematoda: Anisakidae) from various hosts in Australia. Parasitology Research 104, 1507–1525.CrossRefGoogle ScholarPubMed
Shamsi, S., Gasser, R. & Beveridge, I. (2011) Mutation scanning-coupled sequencing of nuclear ribosomal DNA spacers (as a taxonomic tool) for the specific identification of different Contracaecum (Nematoda: Anisakidae) larval types. Molecular and Cellular Probes 25, 13–18.CrossRefGoogle ScholarPubMed
Shamsi, S., Gasser, R. & Beveridge, I. (2013) Description and genetic characterisation of Hysterothylacium (Nematoda: Raphidascarididae) larvae parasitic in Australian marine fishes. Parasitology International 62, 320–328.Google Scholar
Shamsi, S., Poupa, A. & Justine, J.-L. (2015) Characterisation of Ascaridoid larvae from marine fish off New Caledonia, with description of new Hysterothylacium larval types XIII and XIV. Parasitology International 64, 397–404.Google Scholar
Shamsi, S., Ghadam, M., Suthar, J., Ebrahimzadeh Mousavi, H., Soltani, M. & Mirzargar, S. (2016) Occurrence of ascaridoid nematodes in selected edible fish from the Persian Gulf and description of Hysterothylacium larval type XV and Hysterothylacium persicum n. sp. (Nematoda: Raphidascarididae). International Journal of Food Microbiology 236, 65–73.CrossRefGoogle Scholar
Thompson, J.D., Gibson, T.J., Plewniac, F., Jeanmougin, F. & Higgins, D.G. (1997) The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 24, 4876–4882.CrossRefGoogle Scholar
Yagi, K., Nagasawa, K., Ishikura, H., Nakagawa, A., Sato, N., Kikuchi, K. & Ishikura, H. (1996) Female worm Hysterothylacium aduncum excreted from human: a case report. Japanese Journal of Parasitology 45, 12–23.Google Scholar
Zhao, J.-Y., Zhao, W.-T., Ali, A.H., Chen, H.-X. & Li, L. (2017) Morphological variability, ultrastructure and molecular characterisation of Hysterothylacium reliquens (Norris & Overstreet, 1975) (Nematoda: Raphidascarididae) from the oriental sole Brachirus orientalis (Bloch & Schneider) (Pleuronectiformes: Soleidae). Parasitology International 66, 831–838.Google Scholar